Research Article

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04/12/2013
Cassava; Essential amino acids; Interspecific hybrids; Protein

Cassava (Manihot esculenta) is a principal food for large populations of poor people in the tropics and subtropics. Its edible roots are poor in protein and lack several essential amino acids. Interspecific hybrids may acquire high protein characteristics from wild species. We analyzed 19 hybrids of M. esculenta with its wild relative, M. oligantha, for crude ... more

P.T.C. Gomes; N.M.A. Nassar
08/12/2013
Allopolyploids; GISH; Intergeneric hybrids; Interspecific hybrids; Plant improvement

Genomic in situ hybridization (GISH), which is a modification of fluorescent in situ hybridization, has been widely used in the study of plants. It has become one of the most important techniques for molecular cytogenetics. GISH is a technique that allows distinguishing the genomes in a cell. With this technique, it is possible to differentiate the genomes in a hybrid; ... more

G.S. Silva; M.M. Souza
03/13/2013
Interspecific hybrids; ISSR; Morphological trait; RAPD; Solanum melongena; Solanum torvum

Interspecific hybrids between cultivars of eggplant (Solanum melongena L.) and its wild relative S. torvum, which has disease resistance and desirable traits for crop improvement, were obtained by cross-hybridization and embryo rescue. Twenty-one hybrid progenies were obtained and examined based on morphological traits, RAPD and ISSR markers. Five of them were confirmed ... more

J. Kumchai; Y.C. Wei; C.Y. Lee; F.C. Chen; S.W. Chin
03/26/2014
Crambe abyssinica; Crambe hispanica; Crambe kralikii; Cytogenetics; Interspecific hybrids; morphology

In this paper, interspecific crosses among Crambe abyssinica, Crambe hispanica, and Crambe kralikii were reported. In the C. hispanica x C. abyssinica (H x A) cross, 118 F1 hybrids were produced without embryo rescue, while 5 F1 hybrids were obtained with embryo rescue, when C. hispanica was used as the female ... more

X.Z. Du; B.L. Huang; H. Guan; Z.Y. Li; B.Q. Huang
12/12/2014
Amino acids; Antioxidant potential; cluster analysis; Isozymes; Random amplified polymorphic DNA; Trigonella foenum-graecum L.

In this study, we analyzed the correlation between genetic variation based on random amplified polymorphic DNA (RAPD), acid phosphatase, and glutamate-oxaloacetate transaminase isozymes, and amino acid composition with the antioxidant potential status of 7 wild Trigonella foenum-graecum L. accessions collected from diverse ecogeographical regions. RAPD revealed that 90 DNA ... more

E.A. Haliem; A.A. Al-Huqail
11/11/2006
Cassava; India; Plant breeding; Productivity

South America is responsible for about half of the cassava world production. In the 1970’s productivity of the crop on the continent was about 15 ton/ha, and dropped continuously until reaching 12 ton/ha in 2004. India’s productivity of cassava increased from 10 ton/ha in the 1970’s to 28 ton/ha in 2004. Brazil contributed significantly to improving cassava crops through the Instituto ... more

N.M.A. Nassar
03/24/2009
Cassava; In silico analysis; Inverse polymerase chain reaction; Pt2L4 glutamic acid-rich protein; Root promoter; Transient expression

Cassava is one of the most important tropical food crops for more than 600 million people worldwide. Transgenic technologies can be useful for increasing its nutritional value and its resistance to viral diseases and insect pests. However, tissue-specific promoters that guarantee correct expression of transgenes would be necessary. We used inverse polymerase chain reaction to isolate a ... more

C.R. de Souza; F.J. Aragão; E.C.O. Moreira; C.N.M. Costa; S.B. Nascimento; L.J. Carvalho
06/07/2011
Cassava; Gene expression; Gene walking; promoters

Cassava can be cultivated on impoverished soils with minimum inputs, and its storage roots are a staple food for millions in Africa. However, these roots are low in bioavailable nutrients and in protein content, contain cyanogenic glycosides, and suffer from a very short post-harvest shelf-life, and the plant is susceptible to viral and bacterial diseases prevalent in Africa. The demand ... more

M.A. Gbadegesin; J.R. Beeching
05/15/2012
Cassava; M-AFLP; Manihot species; Microsatellites

We developed molecular markers for cassava based on the microsatellite-amplified fragment length polymorphism (M-AFLP) technique. Twenty primer pairs were developed and used for the analysis of 48 samples of Manihot species, consisting of M. esculenta (33), M. esculenta ssp flabellifolia (3), M. chlorosticta (3), M. carthaginensis (3 ... more

S. Whankaew; S. Sraphet; R. Thaikert; D.R. Smith; K. Triwitayakorn
11/25/2003
Cytogenetic behavior; Gene marker; Interspecific hybrids; Introgression

Controlled and natural hybridization between cassava and wild relatives does occur. Barriers within the genus appear to be weak due to recent evolution of the group. All Manihot species examined cytogenetically have a chromosome number of 2n = 36. However, they behave meiotically as diploids. The weak interspecific barriers have led to an extremely heterozygous gene pool that may begin ... more

N.M.A. Nassar

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