Research Article

Related GMR Articles

Hepatitis B virus; HepG2 cell; Hygromycin; Stable cell line

HepG2.2.15 cell is a widely used cell model for studying HBV (hepatitis B virus) in vitro. In these cells, the HBV genome is integrated in several sites of HepG2 cellular DNA. These multiple copies may have some influence on the cellular processes. We constructed a new plasmid, pSEH-Flag-HBV, and transfected it into HepG2 cells, and then screened it with hygromycin. We then used ... more

L.Y. Wang; Y.G. Li; K. Chen; K. Li; J.L. Qu; D.D. Qin; H. Tang
Gene polymorphisms; Hepatitis B virus; RANTES; Saudi Arabia

Besides the host immune response, genetic and environmental factors play crucial roles in the manifestation of hepatitis B virus (HBV) infection. “Regulated on activation normal T-cell expressed and secreted” factor (RANTES) plays a vital role in CD4+, CD8+ T-lymphocyte and dendritic cell activation and proliferation in inflammation. Single nucleotide polymorphisms ... more

A. Al-Qahtani; S. Alarifi; M. Al-Okail; Z. Hussain; A. Abdo; F. Sanai; M. Al-Anazi; N. Khalaf; H. Al-Humaidan; M. Al-Ahdal; F.N. Almajhdi
Adefovir dipivoxil; Hepatitis B virus; Mutant; Reverse dot hybridization

Early detection of adefovir dipivoxil-resistant mutants during long-term treatment of chronic hepatitis B virus (HBV) infection with this drug is of great clinical importance. We developed an improved reverse dot hybridization test for simple and rapid detection of the rtA181V/T and rtN236T mutations associated with adefovir dipivoxil resistance in chronic hepatitis B patients. Probes ... more

Y. Hu; W.L. Zhang; S.L. Xie; Y. Zhao; J.L. Hu; X.F. Cai; G.Q. Lai; A.L. Huang
Anti-gene therapy; Hepatitis B virus; Liposome; Locked nucleic acid

The aim of this study was to investigate the effects of inhibition of the hepatitis B virus (HBV) S gene by polypurine region locked nucleic acid on viral replication in cells. We designed and synthe­sized a locked nucleic acid, phosphorothioate oligonucleotides, unmod­ified oligonucleotides, and unrelated control sequence for the hepatitis B virus S gene polypurine ... more

Y.B. Deng; H.J. Qin; Y.H. Luo; Z.R. Liang; J.J. Zou
Bilirubin; Hepatitis B virus; Hepatocellular carcinoma; UGT1A1 polymorphism

Hepatitis B virus (HBV) is the infectious agent of both acute and chronic hepatitis. HBV exists in multiple genotypic variants that differ in their capacity to become persistent chronic infections and in their clinical manifestations, including hepatocellular carcinoma. The 8 genotypes (A-H) of HBV show a specific worldwide geographic distribution and are correlated with different disease ... more

E. Marku; P.E. Maltese; M. Koni; N. Capodicasa; I.S. Qendro; E. Rigoni; S. Cecchin; M. Bertelli
Chronic hepatitis B; Hepatocellular carcinoma; miR-122; miR-29

MicroRNA molecules have been increasingly regarded as a diagnostic and prognostic marker of certain diseases. The aim of this study was to investigate the expression and clinical significance of miR-122 and miR-29 in liver disease related to hepatitis B virus infection. The serum levels of miR-122 and miR-29 in 20 patients with hepatocellular carcinoma (HCC), 20 patients with liver ... more

T.J. Xing; D.F. Jiang; J.X. Huang; Z.L. Xu
Association; Cattle; PRDM16 gene; Sequence variants

As a zinc-finger protein, PR domain containing 16 (PRDM16) controls brown fat determination by stimulating brown fat cell production while suppressing the expression of genes for production of white fat cells; mutations in this domain are associated with myelodysplastic syndrome and leukemogenesis. In our study, polymorphisms in exons 2, 3, 4, 5, 7, 8, and 9 of the PRDM16 gene ... more

J. Wang; C. Wang; R. Tian; Y.Z. Huang; X.S. Lai; X.Y. Lan; J.Q. Wang; H. Chen