Research Article

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Conservation; Endangered species; Mitochondrial DNA; Oryx; Phylogenetic relationship

The present study reports the application of mitochondrial markers for the molecular phylogeny of Oryx species, including the Arabian oryx (AO), scimitar-horned oryx (SHO) and plains oryx (PO), using the Addax as an outgroup. Sequences of three molecular markers, 16S rRNA, cytochrome b and a control region, for the above four taxa were aligned and the topologies of respective ... more

H.A. Khan; I.A. Arif; A.A.Al Homaidan; A.H.Al Farhan
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bottleneck; Endangered species; Microsatellite marker; Tanichthys albonubes

We developed 12 microsatellite loci for the endangered minnow species, Tanichthys albonubes, using PCR-based isolation of microsatellite arrays. These new markers were tested in 26 individuals from a wild population collected from Guangzhou in China and 26 individuals from a cultured strain. The number of alleles ranged from two to nine and the expected heterozygosity from 0.177 ... more

J. Zhao; D.Y. Tseng; H.D. Lin; X.T. Lin
10/10/2013
Dominant marker; Endangered species; Fragmentation; Genetic structure; Tropical forest

We analyzed the genetic diversity of populations of two sympatric species of Lymania (Bromeliaceae), both endemic to the Atlantic rainforest of southern Bahia (Brazil). Lymania azurea has a restricted occurrence, while Lymania smithii has a wider distribution. Our aim was to provide genetic data to contribute to the design of more efficient conservation ... more

V.C.C. Pamponét; T.F. Alves; R.A. Martinez; R.X. Corrêa; F.A. Gaiotto
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Annamocarya sinensis; Endangered species; Genetic diversity; Microsatellite; Population structure

The endangered perennial plant Annamocarya sinensis (Dode) Leroy is a tertiary relict tree restricted to southeastern China and northern Vietnam. To explore endangerment mechanisms, develop protection strategies, and guide reintroduction efforts for this species, we investigated genetic diversity and population structure by surveying 70 individuals from three distinct ... more

Z.Y. Zhang; X.M. Pang; J.W. Han; Y. Wang; Y.Y. Li
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Endangered species; Genetic diversity; Mergus squamatus; Wintering habitat

The scaly-sided merganser (Mergus squamatus), found in temperate East Asia, has been reduced to a very small population. Central and southern China are its main wintering habitat. However, populations have declined greatly since the 1980s due to habitat loss and degradation, and poaching. To meet the urgent need for up-to-date conservation information, we examined RAPD DNA ... more

Z.Q. Zhang; C.J. Qin; D.A. Fang; A.P. Wang; C.H. Luo; K. Liu
05/22/2012
Atlantic rainforest; DNA extraction protocol optimization; Endangered species; Molecular marker

Melanoxylon brauna (Fabaceae - Caesalpinioideae) is an endemic and valuable hardwood tree species in the Brazilian Atlantic rainforest; it is comparable to African ebony wood. We tested three protocols of DNA extraction based on the citrimonium bromide (CTAB) method and evaluated the quantity, purity and integrity of the DNA. We also determined whether these procedures interfere ... more

D.B. Borges; M.B. Amorim; A.M. Waldschmidt; E. Mariano-Neto; C.V. Vivas; D.G. Pereira
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Allozymes; Conservation; Elaeagnus mollis; Endangered species; Population genetic structure

Elaeagnus mollis Diels is a group of shrubs and dwarf trees endemic to China and are endangered plants. However, the reason why these plants are endangered remains controversial. The current study aimed to explore the endangered status of E. mollis from a genetic perspective and to propose conservation strategies for this species. Using 16 polymorphic allozyme loci, the ... more

J.M. Zhang; F. Zhang
04/27/2015
Chloroplast simple sequence repeat; Endangered species; Genetic diversity; Inbreeding depression; Outbreeding depression

To understand the genetic mechanisms underlying the endangerment of Pinus koraiensis, we studied the mating system of 49 families of this species in 3 natural populations along its post-glacial colonization route across ~1500 km in northeastern China using the chloroplast simple sequence repeat technique. We analyzed 11 polymorphic loci with clear and repeating bands, and we ... more

X. Sui; F.J. Feng; D. Zhao; M. Xing; X.Y. Sun; S.J. Han; M.H. Li
04/27/2015
Endangered species; Microsatellites; Next-generation sequencing; Red fox; Vulpes vulpes

The red fox, Vulpes vulpes (Canidae), is the most widely distributed terrestrial carnivore worldwide, but this species is classified as endangered in Korea. In this study, we developed 25 polymorphic microsatellite markers that included 3-13 (mean = 6.32) alleles per locus using 22 red fox individuals. The most polymorphic locus was FR(59)TG (13 alleles ... more

J.N. Yu; C.U. Chung; K.H. Oh; B.K. Lee; C.E. Lim
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Ctenomyidae; Cytochrome b; Evolution; Molecular phylogeny

The genus Ctenomys (Rodentia: Ctenomyidae) shows several taxonomic inconsistencies. In this study, we used an integrative approach including DNA sequences, karyotypes, and geometric morphometrics to evaluate the taxonomic validity of a nominal species, Ctenomys bicolor, which was described based on only one specimen in 1912 by Miranda Ribeiro, and since then neglected. ... more

J.F.B. Stolz; G.L. Gonçalves; L. Leipnitz; T.R.O. Freitas

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