Research Article

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12/13/2013
Embryos; Expression pattern; Pectoral muscle; Pekin duck; Tissue sections

To confirm the entire developmental process and transition point of embryonic Pekin duck pectoral muscle, and to investigate the association between pectoral muscle development and their regulating genes, anatomical and morphological analyses of embryonic Pekin duck skeletal muscles were performed, and the expression patterns of its regulating genes were investigated. The anatomical ... more

L.H. Gu; T.S. Xu; W. Huang; M. Xie; W.B. Shi; S.D. Sun; S.S. Hou
05/06/2013
Expression pattern; Insulin-like growth factors; Muscle tissue

Insulin-like growth factors (IGFs) are regulators that modulate the proliferation and differentiation of muscle tissues. We quantified the messenger RNA (mRNA) expression of IGF-I, IGF-II, and type I and II IGF receptors (IGF-IR and IGF-IIR) in muscle tissues including the breast, leg, and myocardium during an early postnatal development growth stage (post-hatching weeks 1-8) in ducks. ... more

C.L. Song; H.H. Liu; J. Kou; L. Lv; L. Li; W.X. Wang; J.W. Wang
04/02/2013
Characterization; Cloning; Expression pattern; Spodoptera litura; Trehalase

The oriental leafworm moth, Spodoptera litura, is a major agricultural pest in southeast Asia and nearby Pacific regions. Two distinct trehalases have been identified in insects: soluble trehalase (Treh1) and membrane-bound trehalase (Treh2), although there is currently no information on these genes in S. litura. To characterize the distribution and function of treh ... more

Q. Zou; P. Wei; Q. Xu; H.Z. Zheng; B. Tang; S.G. Wang
10/10/2013
Gene cloning; MID1IP1; Porcine; Prokaryotic expression

We amplified S14R protein gene cDNA of porcine, cloned it into a prokaryotic expression plasmid, and expressed it in Escherichia coli. A pair of primers was designed based on the cDNA sequence of the porcine S14R gene in GenBank. The target gene fragment from porcine liver tissue was amplified by RT-PCR. Confirmed by auto-sequencing, the target gene fragment was ... more

Y.J. Guo; G.Z. Liu; C.M. Wang; Y.Y. Wang; H.J. Li; K. Zhong; W.F. Lu; Y.L. Wang; G.Y. Yang
12/04/2014
FoxO1; MyHC isoforms; Porcine; Skeletal muscle

We examined the expression of myosin heavy chain (MyHC) isoforms and forkhead box transcription factor O1 (FoxO1) in porcine soleus and extensor digitorum longus (EDL) muscles to clarify the correlation of FoxO1 and the relative abundance of transcripts of MyHC isoforms. Soleus muscle was found to be redder than EDL muscles in pigs, and immunohistochemical fast MyHC staining showed more ... more

X.E. Shi; Z.Y. Song; Q.M. Yang; Y.G. Liu; G.S. Yang
02/28/2014
Polymorphism; Porcine; Receptor function; TLR3

Toll-like receptor 3 (TLR3) recognizes double-stranded RNA, which is a molecular signature of viruses, and plays a pivotal role in host defense against viral invasion. Polymorphisms in the human TLR3 gene have been shown to affect the receptor function and to be associated with a variety of diseases, suggesting correlations between TLR3 polymorphisms and the disease resistance/ ... more

L. Wang; Y.C. Chen; D.J. Zhang; H.T. Li; D. Liu; X.Q. Yang
02/27/2014
Association analysis; Carcass traits; Polymerase chain reaction-restriction fragment length polymorphism; Porcine; Titin immunoglobulin domain protein (TTID)

The titin immunoglobulin domain (TTID) protein localizes to the Z line in muscle and binds to alpha-actinin and gamma-filamin. It plays an indispensable role in stabilizing and anchoring of thin filaments. In this study, the 5'-regulatory region of the porcine TTID gene was analyzed with bioinformatic methods. Another objective of this study was to further investigate the ... more

J. Wang; Y.P. Feng; B. Zuo; Y.Z. Xiong; C.Y. Deng
02/06/2015
Bactericidal/permeability-increasing protein; Escherichia coli F18; Expression system; Porcine

The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recom­binant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expression was detected in yeast GS115, ... more

Z.C. Wu; Y. Liu; W.H. Dong; S.Y. Sun; G.Q. Zhu; S.L. Wu; W.B. Bao
07/27/2015
Apoptosis; cFLIP; Corpus albicans; Corpus luteum; Porcine

We determined expression and localization of the anti-apoptotic cellular FLICE inhibitory protein (cFLIP) in the porcine corpora lutea (CL) and corpus albicans (CA) during estrous and preg­nancy. The CL and CA were collected at different stages of estrous to determine cFLIP immunolocalization, and mRNA and protein ex­pression. The mRNA expression of the short cFLIP isoform (cFLIPS ... more

H.Z. Jin; W.S. Shi; Y. Tian; Y. Liu; Y. Jin; N. Manabe
11/23/2015
Lipasin cloning; Porcine; Prokaryotic expression

Lipasin has recently been demonstrated to be involved in lipid metabolism. In this study, two specific primers were used to amplify the lipasin open reading frame from porcine liver tissue. The polymerase chain reaction product was cloned to a pGEM®-T Easy Vector, digested by SalI and NotI, and sequenced. The lipasin fragment was then cloned to a pET21(b) vector and ... more

M.M. Li; J. Geng; Y.J. Guo; X.Q. Jiao; W.F. Lu; H.S. Zhu; Y.Y. Wang; G.Y. Yang

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