Research Article

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03/24/2014
Genome; Simple sequence repeat; Siniperca kneri Garman; Transcriptome

In this study, 37 transcriptome-derived simple sequence repeat (SSR) markers and 18 genomic SSR markers were developed and characterized in the Chinese perch, Siniperca kneri Garman. The average allele number per locus was 5.1 (range: 2-8) for transcriptome-derived SSRs and 3.8 (range: 2-5) for genomic SSRs. The average observed and expected heterozygosities were 0.666 (range: 0. ... more

M. Yang; H.Z. Zheng; X.F. Liang; C.X. Tian; Y.Q. Dou; K.C. Zhu; Y.C. Yuan
04/08/2014
Genotyping; High-resolution melting; Internal oligonucleotide calibrators; Limitations; Simple sequence repeat

Variation in microsatellite or simple sequence repeat (SSR) loci has, until recently, relied heavily on the use of gel-based methods that can be both time consuming and difficult to genotype. Non gel-based systems are therefore important to increase simplicity and improve turn-around time without compromising assay sensitivity and accuracy. In this report, we assessed the latest of the ... more

M. Yang; Y.J. Yue; T.T. Guo; J.L. Han; J.B. Liu; J. Guo; X.P. Sun; R.L. Feng; Y.Y. Wu; C.F. Wang; L.P. Wang; B.H. Yang
04/08/2014
Dendrobium; Internal transcribed spacer; Molecular marker; Simple sequence repeat

The Orchidaceae is one of the largest and most diverse families of flowering plants. The Dendrobium genus has high economic potential as ornamental plants and for medicinal purposes. In addition, the species of this genus are able to produce large crops. However, many Dendrobium varieties are very similar in outward appearance, making it difficult to distinguish one ... more

Y.T. Liu; R.K. Chen; S.J. Lin; Y.C. Chen; S.W. Chin; F.C. Chen; C.Y. Lee
06/11/2015
cluster analysis; Core collection; Germplasm; Malus domestica; Simple sequence repeat

Several types of information can be used to select core collections, including passport data, agronomic data, and molecular data. However, little is known about the ability of core collections to retain the genetic diversity and structure of the whole collection for characters that were not considered during the selection, particularly when molecular markers are used. In this study, two ... more

W.H. Yun; S.H. Ban; G.H. Kim; J.H. Kim; S.I. Kwon; C. Choi
06/12/2015
Holothuria leucospilota; Magnetic bead enrichment; Polymorphic microsatellite loci; Simple sequence repeat

The sea cucumber Holothuria leucospilota has high me­dicinal value and rich nutritional edible value, and thus is a commercially important aquatic product in China. Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. In this study, 16 novel polymorphic microsatellite markers ... more

J.B. Shangguan; Z.B. Li; Y.F. Ning; Y.S. Huang; Y. Yuan; J. Lu; B.B. Li; X.Q. Mao
05/18/2015
Population genetics; Qinghai-Tibet Plateau; Rhodiola alsia; Simple sequence repeat

Rhodiola alsia, which has been used widely in traditional Chinese medicine for a considerable time, grows on moist habitats at high altitude near the snow line. Microsatellite loci were developed for R. alsia to investigate its population genetics. In total, 17 polymorphic microsatellites were developed based on ESTs from the Illumina HiSeqTM 2000 platform. ... more

F.Q. Zhang; S.Y. Lei; Q.B. Gao; G. Khan; R. Xing; H.L. Yang; S.L. Chen
04/30/2015
Amplified fragment length polymorphism; Bulked segregant analysis; Lagerstroemia; Plant architecture; Simple sequence repeat

Plant height is one of the most important traits of plant architecture as it modulates both economic and ornamental values. Crape myrtle (Lagerstroemia indica L.) is a popular ornamental woody plant because of its long-lasting mid-summer bloom, rich colors, and diversified plant architecture. These traits also make it an ideal model of woody species for genetic analysis of many ... more

Y.J. Ye; Y. Liu; M. Cai; D. He; J.S. Shen; Y.Q. Ju; X.M. Bian; H.T. Pan; Q.X. Zhang
03/27/2015
Association mapping; Fatty acids; genetic relationships; Olive (Olea europaea L.); Simple sequence repeat; Structure analysis

The purpose of this research was to characterize an olive core collection using some agronomic characters and simple sequence repeat (SSR) markers and to determine SSR markers associated with the content of fatty acids in olive oil. SSR marker analysis demonstrated the presence of a high amount of genetic variation between the olive cultivars analyzed. A UPGMA dendrogram demonstrated that ... more

M. Ipek; A. Ipek; M. Seker; M.K. Gul
03/30/2015
All hermaphrodites; Phylogenetic analysis; Polymorphism; Simple sequence repeat

The papaya (Carica papaya L.) is one of the most important economic tropical fruits in the world, and the hermaphrodite is the preferred type in field cultures. We analyzed the sexual ratio of offspring from the cultivar ‘Taiwan Seed Station No. 7’ (T7) by a self-cross and its cross with Taichung Sunrise (TS). Female progeny from the T7 self-crossing were not observed. This ... more

C.T. Chiu; C.W. Wang; F.C. Chen; S.W. Chin; C.C. Liu; M.J. Lee; W.C. Chung; Y.W. Chien; H.J. Chang; C.Y. Lee
02/13/2015
Quantitative trait loci; Rice; Seed-shattering genes; Sequence characterized amplified region marker; Simple sequence repeat

Seed shattering of wild plant species is thought to be an adaptive trait to facilitate seed dispersal. For rice breeding, seed shatter­ing is an important trait for improving breeding strategies, particularly when developing lines use interspecific hybrids and introgression of genes from wild species. We developed F3:4 recombinant inbred lines from an interspecific cross ... more

S.J. Kwon; J. Yu; Y.J. Park; J.H. Son; N.S. Kim; J.K. Lee

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