Research Article

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10/09/2012
HAPE; Haplogroup; Haplotype; Hypoxia; Mitochondrial DNA; mtSNP

High-altitude pulmonary edema (HAPE) is a life-threatening condition caused by acute exposure to high altitude. Accumulating evidence suggests that genetic factors play an important role in the etiology of HAPE. However, conclusions from association studies have been hindered by limited sample size due to the rareness of this disease. It is known that mitochondria are critical for hypoxic ... more

Y.J. Luo; W.X. Gao; S.Z. Li; X.W. Huang; Y. Chen; F.Y. Liu; Q.Y. Huang; Y.Q. Gao
10/27/2014
Adipose-derived stem cells; Bone defect; Bone morphogenetic protein 2; Osteogenic differentiation; Osteoporosis

Osteoporosis poses a major public health threat in aging societies. Adipose-derived stem cells (ADSCs) are multipotent adult stem cells that have the ability to yield mesenchymal stem cells, and have the potential to undergo osteogenesis and bone regeneration. Bone morphogenetic proteins (BMPs) have been demonstrated to upregulate bone gene expression after mechanical injury and to ... more

H.L. Fu; Z.Y. Diao; L. Shao; D.P. Yang
09/16/2011
cDNA array; Demineralized bone matrix; Gene expression; Human periosteal cells; Osteogenesis

Demineralized bone matrix (DBM) has been widely investigated as a biomaterial to promote new bone formation and is utilized clinically for bone repair and regeneration. We investigated gene expression patterns of osteogenic differentiation in human periosteal (HPO) cells cultured with demineralized bone matrix, using cDNA array technology. Osteogenic differentiation of HPO cells was ... more

S. Honsawek; P. Bumrungpanichthaworn; T. Thitiset; L. Wolfinbarger
2/16/2017
Adipose tissue; Animals; Bone morphogenetic protein 2; Cell differentiation; Cells, Cultured; Collagen type I; Osteoblasts; Osteocalcin; Osteogenesis; Polyethyleneimine; Rabbits; Stem cells; Tissue engineering; Transfection

The aim of this study was to examine the efficiency of polyethylenimine-mediated transfection of the human bone morphogenetic protein-2 (BMP-2) gene into rabbit adipose-derived stem cells (ADSCs), and its effect on osteoblast differentiation. Adipose tissue was isolated from the necks of adult Japanese white rabbits and cultured in vitro to obtain ADSCs. Gene delivery of BMP-2 was mediated by ... more

Q.F. Wang; Y. Huang; G.C. He; H.S. Wang; Z.H. Chen; X.H. Cai; Y.H. Xie; Q. Liu
11/16/2010
Gene expression analysis; Id1; mRNA; PBMCs; Proliferation; Psoriasis

Although the precise causes of psoriasis are unclear, it is widely accepted that psoriasis is a disorder in which factors in the immune system, enzymes, and other biochemical substances that regulate skin cell division are impaired, leading to rapid proliferation of keratinocytes and incomplete keratinization. Expression of the helix-loop-helix transcription factor Id1 (inhibitor of ... more

C. Ronpirin; M. Achariyakul; T. Tencomnao; J. Wongpiyabovorn; W. Chaicumpa
10/17/2013
Esophageal cancer; Flow cytometry; Gene transfection; MT-1E; MT-3; Proliferation

Metallothionein (MT)-3 has cell growth inhibitory activity, and is the only currently known MT subtype with unique physiological functions. The expression levels of MT-1E, a subtype of MT-1, were positively correlated with the degree of esophageal cancer malignancy. The present study aimed to investigate the effects of MT-3 and MT-1E gene transfection on the proliferation, cell cycle, and ... more

Z.Q. Tian; Y.Z. Xu; Y.F. Zhang; G.F. Ma; M. He; G.Y. Wang
04/02/2013
Apoptosis; Invasion; miR-181b; PC-3; Proliferation; Prostate cancer

We examined microRNA-181b (miRNA) expression in prostate cancer tissues and its effect on the prostate cancer cell line PC-3. Tissues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected by surgical removal. Total miRNA was extracted, and the relative expression of miR-181b was quantified using RT-PCR. miR-181b ASO was transfected into prostate cancer PC ... more

L. He; H. Yao; L.H. Fan; L. Liu; S. Qiu; X. Li; J.P. Gao; C.Q. Hao
05/15/2012
NANOG; Neural differentiation; NT2/D1; OCT4; Proliferation; SOX2

SOX2, a universal marker of pluripotent stem cells, is a transcription factor that helps control embryonic development in vertebrates; its expression persists in neural stem/progenitor cells into adulthood. Considering the critical role of the SOX2 transcription factor in the regulation of genes required for self-renewal and pluripotency of stem cells, we developed and ... more

D. Drakulic; A. Krstic; M. Stevanovic
04/14/2014
Anchor-independent growth; Colorectal cancer LoVo cell; ETS-1; LINE-1 ORF-1p; Proliferation; Transcriptional activation

The human proto-oncogene long interspersed nucleotide acid element-1 (LINE-1) open reading frame-1 protein (ORF-1p) is involved in the progress of several cancers. The transcription factor ETS-1 can mediate the transcription of some downstream genes that play specific roles in the regulation of cancerous cell invasion and metastasis. In this study, the effects of LINE-1 ORF-1p on ETS-1 ... more

M.Y. Li; M. Zhu; F. Feng; F.Y. Cai; K.C. Fan; H. Jiang; Z.Q. Wang; E.Q. Linghu
08/25/2014
Mesenchymal stem cell; Osteoblast differentiation; Proliferation; Rifampicin

This study was designed to investigate the effect of different concentrations of rifampicin on osteogenic differentiation and proliferation of mesenchymal stem cells (MSCs) in human bone marrow. Rifampicin treatment at 0, 4, 8, 16, 32, 64, and 128 mg/mL was applied throughout the whole process, from stromal cells purified from human bone marrow to differentiated bone cells. The effect of ... more

Z. Zhang; X. Wang; F. Luo; H. Yang; T. Hou; Q. Zhou; F. Dai; Q. He; J. Xu

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