Research Article

Related GMR Articles

10/10/2013
Gene cloning; MID1IP1; Porcine; Prokaryotic expression

We amplified S14R protein gene cDNA of porcine, cloned it into a prokaryotic expression plasmid, and expressed it in Escherichia coli. A pair of primers was designed based on the cDNA sequence of the porcine S14R gene in GenBank. The target gene fragment from porcine liver tissue was amplified by RT-PCR. Confirmed by auto-sequencing, the target gene fragment was ... more

Y.J. Guo; G.Z. Liu; C.M. Wang; Y.Y. Wang; H.J. Li; K. Zhong; W.F. Lu; Y.L. Wang; G.Y. Yang
11/27/2014
Expression pattern; Muscle growth; Porcine; qRT-PCR

Muscle growth and development is associated with remarkable changes in protein-coding and microRNA (miRNA) gene expression. To determine the expression patterns of genes and miRNAs related to muscle growth and development, we measured the expression levels of 25 protein-coding and 16 miRNA genes in skeletal and cardiac muscles throughout 5 developmental stages by quantitative reverse ... more

F.J. Wang; L. Jin; Y.Q. Guo; R. Liu; M.N. He; M.Z. Li; X.W. Li
02/28/2014
Polymorphism; Porcine; Receptor function; TLR3

Toll-like receptor 3 (TLR3) recognizes double-stranded RNA, which is a molecular signature of viruses, and plays a pivotal role in host defense against viral invasion. Polymorphisms in the human TLR3 gene have been shown to affect the receptor function and to be associated with a variety of diseases, suggesting correlations between TLR3 polymorphisms and the disease resistance/ ... more

L. Wang; Y.C. Chen; D.J. Zhang; H.T. Li; D. Liu; X.Q. Yang
02/27/2014
Association analysis; Carcass traits; Polymerase chain reaction-restriction fragment length polymorphism; Porcine; Titin immunoglobulin domain protein (TTID)

The titin immunoglobulin domain (TTID) protein localizes to the Z line in muscle and binds to alpha-actinin and gamma-filamin. It plays an indispensable role in stabilizing and anchoring of thin filaments. In this study, the 5'-regulatory region of the porcine TTID gene was analyzed with bioinformatic methods. Another objective of this study was to further investigate the ... more

J. Wang; Y.P. Feng; B. Zuo; Y.Z. Xiong; C.Y. Deng
02/06/2015
Bactericidal/permeability-increasing protein; Escherichia coli F18; Expression system; Porcine

The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recom­binant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expression was detected in yeast GS115, ... more

Z.C. Wu; Y. Liu; W.H. Dong; S.Y. Sun; G.Q. Zhu; S.L. Wu; W.B. Bao
07/27/2015
Apoptosis; cFLIP; Corpus albicans; Corpus luteum; Porcine

We determined expression and localization of the anti-apoptotic cellular FLICE inhibitory protein (cFLIP) in the porcine corpora lutea (CL) and corpus albicans (CA) during estrous and preg­nancy. The CL and CA were collected at different stages of estrous to determine cFLIP immunolocalization, and mRNA and protein ex­pression. The mRNA expression of the short cFLIP isoform (cFLIPS ... more

H.Z. Jin; W.S. Shi; Y. Tian; Y. Liu; Y. Jin; N. Manabe
11/23/2015
Lipasin cloning; Porcine; Prokaryotic expression

Lipasin has recently been demonstrated to be involved in lipid metabolism. In this study, two specific primers were used to amplify the lipasin open reading frame from porcine liver tissue. The polymerase chain reaction product was cloned to a pGEM®-T Easy Vector, digested by SalI and NotI, and sequenced. The lipasin fragment was then cloned to a pET21(b) vector and ... more

M.M. Li; J. Geng; Y.J. Guo; X.Q. Jiao; W.F. Lu; H.S. Zhu; Y.Y. Wang; G.Y. Yang
02/02/2010
Gallus; Gene expression; Skeletal muscle; Tissue-specific expression

Macro- and microarrays are well-established technologies to determine gene functions through repeated measurements of transcript abundance. We constructed a chicken skeletal muscle-associated array based on a muscle-specific EST database, which was used to generate a tissue expression dataset of ~4500 chicken genes across 5 adult tissues (skeletal muscle, heart, liver, brain, and skin). ... more

E.C. Jorge; C.M.R. Melo; M.F. Rosário; J.R.S. Rossi; M.C. Ledur; A.S.A.M.T. Moura; L.L. Coutinho
07/24/2014
Dietary intake; IGF-І; IGF-ІІ; MSTN; Sheep; Skeletal muscle

MSTN, IGF-І(insulin-like growth factor-І) and IGF-II (insulin-like growth factor-II) regulate skeletal muscle growth. This study investigated the effects of different dietary intake levels on skeletal muscles. Sheep was randomly assigned to 3 feeding groups: 1) the maintenance diet (M), 2) 1.4 x the maintenance diet (1.4M), and 3) 2.15 x the maintenance ... more

H.J. Xing; Z.Y. Wang; B.S. Zhong; S.J. Ying; H.T. Nie; Z.R. Zhou; Y.X. Fan; F. Wang
03/31/2015
Gene function; PGM1; Pig; Promoter region; Skeletal muscle

The phosphoglucomutase 1 (PGM1) gene was differentially expressed in tissues of Chinese Meishan and Large White pigs. In this study, the promoter region, expression profile, and genetic mutations of the gene were determined. Expression of a 5'-deletion in both C2C12 and PK-15 cells showed that a negative regulatory element was at -1871 to +185 bp and a positive regulatory ... more

J.Y. Yu; S.M. Shao; Y.Z. Xiong

Pages