Research Article

Related GMR Articles

02/14/2014
Chicken; Ligase detection reaction; Myofiber type; PGC-1α gene; Polymorphism; SNPs

The peroxisome proliferators-activated receptor-γ coactivator-1α (PGC-1α) is a candidate gene for meat quality traits because of its prominent role in muscle fiber type switching and determination. We investigated the effects of the PGC-1α gene on chicken skeletal muscle fiber type switching and on other meat quality traits. Single nucleotide polymorphisms were detected by PCR-SSCP and ... more

J.T. Shu; W.J. Xu; M. Zhang; W.T. Song; Y.J. Shan; C. Song; W.Q. Zhu; X.Y. Zhang; H.F. Li
01/21/2014
Chicken; Newcastle disease virus; Novel gene; Sequence characterization; Transcript expression

The full-length cDNA sequence of a novel expressed sequence tag (GenBank accession No. HQ184338) that was differentially expressed during Newcastle disease virus (NDV) infection in chickens was cloned from the chicken spleen by a rapid amplification of cDNA ends assay. This gene was further analyzed using bioinformatic methods and named grni. The full-length cDNA sequence was ... more

Y. Hua; D.Y. Hui; Z.H. Rong; T. Cheng
06/10/2015
Chicken; GRP78 gene; Heat stress; Quantitative real-time PCR; Single-nucleotide polymorphism

Glucose-regulated protein 78 (GRP78) is a molecular chaperone in the endoplasmic reticulum and can be induced by different kinds of environmental and physiological stress. Thus far, the role of the GRP78 gene in thermotolerance in chickens has not been investigated. In the present study, we detected sequence variations in the 5ꞌ-flanking region of the GRP78 gene and ... more

L.N. Kong; D.X. Zhang; C.L. Ji; X.Q. Zhang; Q.B. Luo
04/30/2015
Chicken; Cloning; Gene expression; Newcastle disease virus; Tumor necrosis factor receptor-associated factor 3

Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a crucial regulator that suppresses c-Jun N-terminal kinase and non-canonical nuclear factor-kB signaling, but facilitates type I interferon production. To determine TRAF3 function in innate immune responses among birds, particularly chicken, we cloned and characterized the chicken TRAF3 gene (chTRAF3) and detected ... more

H.L. Yang; Z.Q. Feng; S.Q. Zeng; S.M. Li; Q. Zhu; Y.P. Liu
04/10/2015
Chicken; Cloning; R-spondin1; Tissue distribution

Rspo1 belongs to the Rspo family, which is composed of 4 members (Rspo1-4) that share 40 to 60% sequence homology and similar domain organizations, and regulate the WNT signaling pathway via a common mechanism. Rspo1 plays a key role in vertebrate development and is an effective mitogenic factor of gastrointestinal epithelial cells. We report the cloning ... more

Y.Q. Han; J. Geng; H.T. Shi; X.M. Zhang; L.L. Du; F.T. Liu; M.M. Li; X.T. Wang; Y.Y. Wang; G.Y. Yang
04/13/2015
Chicken; Host defense peptide; Lipopolysaccharide; Vitamin D3; β-defensin

The objective of this study was to evaluate the effect of vitamin D3 (VD3) on the regulation of chicken intestinal β-defensin genes under normal and lipopolysaccharides (LPS) conditions. Four treatment groups were used, including a negative control group, VD3-injection group, LPS-injection group, and both VD3-injection and LPS-injection group. At ... more

L. Lu; S.M. Li; L. Zhang; X.Q. Liu; D.Y. Li; X.L. Zhao; Y.P. Liu
03/30/2015
Chicken; Full-length cDNA; NF-κB; Nucleotide-binding oligomerization domain-containing protein -1; Receptor-interacting protein 2

Nucleotide-binding oligomerization domain-containing protein-1 (NOD1) is a cytoplasmic pattern recognition receptor (PRR) and a key member of the NOD-like receptor (NLR) family. It has been reported that NLRs recognize a variety of microbial infections to induce the host innate immune response via modulation of NF-κB signaling. However, no reports on chicken NOD1 have been ... more

Z.Y. Tao; C.H. Zhu; Z.H. Shi; C. Song; W.J. Xu; W.T. Song; J.M. Zou; A.J. Qin
02/06/2015
Chicken; Fat-related traits; Gene expression; Housing system

Free-range production system is increasingly being used in poultry breeding and feed production in many countries. The objective of the current experiment was to evaluate the effects of different raising systems on fat-related traits and mRNA levels of liver lipogenesis genes in Erlang Mountainous chicken. Each of 10 birds (91 day old) from caged, indoor-floor housed, and free-range ... more

Q. Li; X.L. Zhao; E.R. Gilbert; Y.P. Liu; Y. Wang; M.H. Qiu; Q. Zhu
03/06/2015
Chicken; Haplotype; HSP70; Single nucleotide polymorphism

This study was designed to detect the sequence variation of the chicken heat shock protein 70 (HSP70) gene. A total of 102 individuals from 8 native Chinese breeds together with Dwarf White Chicken and Red Junglefowl were used to detect sequence variations. The coding regions of the chicken HSP70 gene from 102 individuals were cloned and sequenced. Thirty-six variations ... more

J.K. Gan; L.Y. Jiang; L.N. Kong; X.Q. Zhang; Q.B. Luo
02/13/2015
Calpain (CAPN1); Chicken; Ligase detection reaction; Meat tenderness; Polymorphism

The micromolar calcium-activated neutral protease gene (CAPN1) is a physiological candidate gene for meat tenderness. Four previously identified single nucleotide polymorphism (SNP) markers located within the CAPN1 gene were evaluated for their associations with variation in the meat tenderness of a Chinese indigenous chicken breed, a higher meat quality breed (i.e., ... more

J.T. Shu; M. Zhang; Y.J. Shan; W.J. Xu; K.W. Chen; H.F. Li

Pages