Research Article

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Calmodulin (CALM), a calcium-binding protein, is expressed in the hypothalamic-pituitary-gonadal axis; it plays a pivotal role in the reproductive system by regulating gonadotropin-releasing hormone signaling. Downstream of hypothalamic-pituitary-gonadal signaling pathways, liver receptor homolog-1 (LRH-1) is involved in female gonadal hormone synthesis. In the chicken, although the two ... more

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Chicken; EST; Gallus gallus; Genomics; Microsatellites; SSR

Many years of domestication and breeding have given rise to the wide range of chicken breeds that exist today; however, an increasing number of local chicken breeds are under threat of extinction. A comprehensive characterization of chicken markers (especially type I markers) is needed to monitor and conserve genetic diversity in this species. The explosion of genomics and functional ... more

M.R. Bakhtiarizadeh; B. Arefnejad; E. Ebrahimie; M. Ebrahimi
04/19/2012
Abdominal fat traits; Body weight; Chicken; Microsatellite marker; NEAURP; Quantitative trait loci

Body weight and abdominal fat traits in meat-type chickens are complex and economically important factors. Our objective was to identify quantitative trait loci (QTL) responsible for body weight and abdominal fat traits in broiler chickens. The Northeast Agricultural University Resource Population (NEAURP) is a cross between broiler sires and Baier layer dams. We measured body weight and ... more

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Chicken; Heat shock factor-3; Heat shock protein 70; Heat treatment

This study aimed to estimate changes in HSF3 and HSP70 mRNA expression in stress-sensitive tissues of 2 chicken breeds during acute heat stress. Lingshan chickens (LSC) and White Recessive Rock (WRR) (24 chickens of each breed) were randomly divided into 4 groups (0, 2, 3, and 6 h of heat treatment). With increasing heat treatment time, both HSF3 and HSP70 ... more

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In this study, chicken adipocytes were cultured to evaluate RNA interference by the leptin receptor gene. A small interfering RNA of the leptin receptor gene was synthesized, with a suppression rate of 60% being generated (P peroxisome proliferator-activated receptor γ, fatty acid synthase, adipose triglyceride lipase, and lipoprotein ... more

A.X. Huang; J.J. Li; Y. Tian; J.D. Shen; Z.R. Tao; G.Q. Li; L.Z. Lu; Y. Fu; T.X. Wu
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Chicken; Egg production; Growth hormone gene; Ligase detection reaction; Polymorphism

Growth hormone (GH) has diverse functions in animals, together with other hormones from the somatotropic axis. Here, chicken GH (cGH) was investigated in recessive white chickens and Qingyuan partridge chickens as a candidate gene affecting egg production traits. Chicken egg production traits were studied in association with 4 selected single nucleotide polymorphisms (T185G, G662A, ... more

Y.J. Su; J.T. Shu; M. Zhang; X.Y. Zhang; Y.J. Shan; G.H. Li; J.M. Yin; W.T. Song; H.F. Li; G.P. Zhao
04/29/2014
Chicken; Genetic diversity; Microsatellite DNA; Red Junglefowl; Yunnan

Yunnan is situated in the Southwest China and encompasses regions having high biodiversity, including habitats for several ancestral species of domestic animals such as chicken. Domestic chickens in Yunnan were kept by peoples of varied ethnic and economic backgrounds living in highly varied geographic environments. To identify the genetic background of Yunnan domestic chickens and their ... more

J.L. Huo; G.S. Wu; T. Chen; H.L. Huo; F. Yuan; L.X. Liu; C.R. Ge; Y.W. Miao
04/29/2014
Chicken; FADS2; Fatty acid; Growth traits; Haplotype; Single nucleotide polymorphisms

Delta-6 fatty acid desaturases are rate-limiting desaturases involved in metabolic processes of fatty acids, and they are encoded by the FADS2 gene. In the current study, an F2 resource population of Gushi chickens crossed with Anak broilers was used to investigate the genetic effects of the chicken FADS2. Two adjacent single nucleotide polymorphisms (SNPs) ( ... more

S.K. Zhu; Y.D. Tian; S. Zhang; Q.X. Chen; Q.Y. Wang; R.L. Han; X.T. Kang
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Chicken; Gonad development; miRNAs; Sexual differentiation

MicroRNAs (miRNAs) are small non-coding RNA molecules that play key roles in the regulation of development processes of many tissues and organs at the post-transcriptional level. However, little is known about how they affect chicken gonadal development. We examined the expression of four miRNAs (miR-218, -200b, -196, and -206) in chicken embryonic gonads at embryonic days 3.5-6.5. Their ... more

Y.P. Feng; J.F. Chen; P. Huang; X. Wang; J. Wang; X.L. Peng; Y.Z. Gong
02/13/2014
Chicken; Development; Dosage compensation; Epigenetics; Gonad

The chicken (Gallus gallus) embryo has been used as a classic model system for developmental studies because of its easy accessibility for surgical manipulation during embryonic development. Sex determination in birds is chromosomally based (ZZ for males and ZW for females); however, the basic mechanism of sex determination is still unknown. Here, the dynamics of expression of ... more

L.C. Caetano; F.G.O. Gennaro; K. Coelho; F.M. Araújo; R.A. Vila; A. Araújo; deMelo Bernardo; C.R. Marcondes; S.M.Chuva de S. Lopes; E.S. Ramos

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