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Related GMR Articles

09/12/2012
Cyprinus carpio L.; Genetic differentiation; Microsatellite; mtDNA; Selection pressure

Selection pressures are the principle evolutionary forces for the genetic differentiation of populations. Recent changes in selection pressures on mitochondrial DNA and microsatellite have been described in a wide variety of organisms. The common carp (Cyprinus carpio) has experienced strong selection pressure, in particular artificial selection, during its domestication. However ... more

L.H. Xu; C.H. Wang; J. Wang; Z.J. Dong; Y.Q. Ma; X.X. Yang
07/19/2012
Genetic diversity; Microsatellite; Molecular breeding; Popcorn; SSR locus; Zea mays

We analyzed genetic structure and diversity among eight populations of popcorn, using SSR loci as genetic markers. Our objectives were to select SSR loci that could be used to estimate genetic diversity within popcorn populations, and to analyze the genetic structure of promising populations with high levels of heterozygosity that could be used in breeding programs. Fifty-seven alleles (3 ... more

I.B.O. Eloi; C.A. Mangolin; C.A. Scapim; C.S. Gonçalves; M.F.P.S. Machado
05/14/2012
Microsatellite; Polymorphism; Taxus chinensis var. mairei

We isolated and characterized 11 microsatellite loci for the Chinese yew, Taxus chinensis var. mairei, an endangered tree species in China, by constructing a (CA)12-enriched library. The number of alleles per locus ranged from 5 to 10. The observed heterozygosities ranged from 0.2500 to 0.8333 and the expected heterozygosities ranged from 0.5196 to 0.8680. No ... more

H. Xue; C.H. Lu; X.B. Wu
02/16/2012
Cyclina sinensis; Genetic diversity; Microsatellite; Polymorphism

The Venus clam, Cyclina sinensis, is one of the most important bivalves in China marine aquaculture. Using (CA)15-enriched genomic libraries of this species, nine novel polymorphic microsatellite loci were isolated and characterized. The mean number of observed alleles per locus was 16 (range 8-24). The observed and expected heterozygosity ranged from 0.119 to 0.872 ... more

J.X. Wang; T.J. Xu; L. Wei; F.X. Meng; R.X. Wang
12/18/2014
Genetic diversity; Microsatellite; Miiuy croaker; Population structure

Genetic diversity and patterns of population structure of the miiuy croaker were investigated using SSR markers. A set of 10 microsatellite loci revealed 40 alleles; the number of alleles varied from 2 to 10 for each marker. A relatively high level of genetic variability was observed between miiuy croaker individuals. Genetic diversity was relatively high within populations with ... more

Y. Qin; D.Q. Sun; T.J. Xu; X.Z. Liu; Y.N. Sun
02/21/2014
Barker frog; Microsatellite; Mitochondrial DNA; Physalaemus; Population structure

The barker frog Physalaemus cuvieri is widely distributed in South America and is found in all regions of Brazil. Significant intraspecific morphological variation in this species has been reported. To determine the genetic structure of the natural Brazilian populations of P. cuvieri, 10 different populations geographically separated by 99.41 to 2936.75 km were evaluated ... more

M. Conte; C.P. Targueta; M.I. Zucchi; A.P. Souza; S.M. Recco-Pimentel
09/12/2014
Effective population size; Microsatellite; Migration; Nong-Han Lake; Whisker sheatfish

Nong-Han Lake, Thailand, sustains the whisker sheatfish (Micronema bleekeri Günther, 1864), which is a rare species of freshwater catfish. Wild-caught whisker sheatfish has been intensively harvested to meet market demand; yet, genetic information about this species remains unknown. To assist with the in situ conservation of whisker sheatfish populations in Nong-Han Lake ... more

P. Phongkaew; U. Arunyawat; A. Swatdipong; V. Hongtrakul
07/25/2014
Huoyan geese; Microsatellite; Molecular marker

In this study, we isolated microsatellite DNA from the Huoyan goose genome with magnetic beads. As a result, 150 positive clones were identified, and 148 microsatellites were found. Among the 148 microsatellites, 69.6% were perfect, 17.6% were imperfect, and the rest were compound type (12.8%). Twenty microsatellite primers were used to screen 90 individuals from 3 Huoyan goose ... more

Z.Z. Cao; D. Su; Y.Y. Zhao; M. Liu; M. Gao; X.H. Luan
05/23/2014
Cervus unicolor; Conservation; Microsatellite; PCR-based isolation of microsatellite arrays; RAPD-PCR enrichment

Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0.750 at each locus. All loci but one, CU18, deviated from Hardy-Weinberg ... more

D.Y. Lin; T.Y. Chiang; C.C. Huang; H.D. Lin; S.J. Tzeng; S.R. Kang; H.M. Sung; M.C. Wu
04/30/2014
Microsatellite; Multiplex; Polyploidy; Puccinellia maritima; Saltmarsh

The grass Puccinellia maritima is an important saltmarsh ecosystem engineer exhibiting wide morphological variation, which is partially genetically determined. Nevertheless, nothing is known about its population genetics or how neutral genetic variation is distributed throughout its geographical range. Here, we describe 12 polymorphic microsatellites pooled into two multiplexes ... more

R. Rouger; M. Vallejo-Marin; A.S. Jump

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