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Related GMR Articles

06/11/2015
Genetic markers; Lunella coronata granulata; Magnetic bead enrichment; Microsatellite

Lunella coronata granulata, from the family Turbini­dae, is an economically important species. The first set of 10 poly­morphic microsatellite loci was screened from L. coronata granulata, and 30 individuals were used to analyze the degree of polymorphism in these loci. The level of observed and expected heterozygosity was 0.0667-0.7333 and 0.0644-0.6628, respectively. ... more

Z.B. Li; Q.H. Li; J.B. Shangguan; Y.F. Ning; G. Dai
05/04/2015
Genetic markers; Magnetic bead enrichment; Megalonibea fusca; Microsatellite

Megalonibea fusca is a commercially important large edible fish. In this study, the first set of 10 polymorphic microsatellite loci for M. fusca was developed and characterized. The number of alleles per locus ranged from two to five, with the observed and expected heterozygosities ranging from 0.0667 to 0.7667, and from 0.0644 to 0.5828, respectively. Most of the loci ... more

Z.B. Li; Q.H. Li; Y.F. Ning; J.B. Shangguan; G. Dai; L.N. Chen; Y.Y. Cao; X.J. Chen
04/17/2015
Atrina vexillum Born; Genetic markers; Magnetic bead enrichment; Microsatellite

The pen shell, Atrina vexillum Born, is an edible shellfish that is widely consumed in the Asia-Pacific region. In this study, 11 polymorphic microsatellite loci were isolated from A. vexillum, and 30 wild individuals were used to evaluate the degree of polymorphism of these markers. The number of alleles per locus ranged from 3 to 8. The polymorphism ... more

Y.S. Huang; Q.H. Li; Z.B. Li; J.B. Shangguan; Y.F. Ning; G. Dai
04/15/2015
Conservation status; Control region mtDNA; Microsatellite; Offshore islands

Although the genus Tursiops has a worldwide distribution and is globally well-studied, some dolphin populations continue to face high risks of decline. Hence, it is necessary to assess the genetic diversity and structure of this genus to properly assess its conservation status and to implement appropriate management actions. In Brazil, genetic studies on this group remain rare, ... more

C.S. Castilho; F. Pedone-Valdez; F. Bertuol; P. Fruet; R.C. Genoves; J.C. Di Tullio; G. Caon; L.S. Hoffmann; T.R.O. Freitas
03/30/2015
Genetic diversity; Genetic structure; Mandarin fish (Siniperca chuatsi); Microsatellite

In this report, 10 polymorphic microsatellites were applied to assess the genetic diversity and genetic differentiation of 5 consecutive breeding generations of mandarin fish, Siniperca chuatsi (Basilewsky). The results from total number of alleles, average polymorphism information content, and average homozygosity and heterozygosity showed that the genetic diversity of the ... more

T.L. Yi; W.J. Guo; X.F. Liang; M. Yang; L.Y. Lv; C.X. Tian; Y. Song; C. Zhao; J. Sun
03/13/2015
Domestic cat; Genetic structure; Microsatellite; Orange tabby cats

We optimized a panel of microsatellite markers from cat and tiger genetic data for efficient genetic monitoring and used it to analyze the genetic structure of an outbred cat stock in China. We selected a set of rich polymorphic microsatellite loci from 131 cat microsatellite loci and 3 Sumatran tiger microsatellite loci using agarose gel electrophoresis. Next, the set of optimized ... more

X.Y. Du; S. Yi; X.Y. Huo; C. Wang; D.F. Liu; W.Z. Ren; Z.W. Chen
02/03/2015
Essential oils; Genetic diversity; Germplasm bank; Lippia alba; Microsatellite

Microsatellite primers were developed and optimized for Lippia alba to characterize the L. alba germplasm bank of Universidade de São Paulo. A genomic library enabled the design of 9 microsatellite primers. Six of the 9 primers yielded polymorphic products, which defined 2 groups in the bank. The data provide support to characterize germplasm banks, genetic breeding ... more

D.S. Rocha; C.P. Santos; M.M. Bajay; J.B. Campos; A.F. Blank; J.B. Pinheiro; M.I. Zucchi
01/30/2015
Allele frequencies; Frequency database; Microsatellite; Sicilian cattle

Short tandem repeats are used as an effective method to trace DNA markers in genotyping. Using a standardized kit, we tested 11 microsatellite markers recommended by the International Society for Animal Genetics (ISAG) in a sample of 495 Sicilian cattle. The aim of this study was to investigate the allele frequencies in the Sicilian cattle population to provide a reference database and ... more

M. Cosenza; S. Reale; T. Lupo; F. Vitale; S. Caracappa
01/15/2015
Fragment length polymorphism of sequences containing repeats; Genetic markers; Microsatellite; Siganus fuscescens

The rabbitfish Siganus fuscescens is an economically valuable species that is widely distributed throughout the estuaries, intertidal, and offshore coasts of the Indo-Pacific and eastern Mediterranean. Ten novel microsatellite loci from the genome of S. fuscescens were developed using the fast isolation protocol with amplified fragment length polymorphism of sequences ... more

Y.F. Ning; Z.B. Li; Q.H. Li; G. Dai; J.B. Shangguan; Y. Yuan; Y.S. Huang
11/08/2011
DNA extraction; Medicinal plant; Molecular study; TriOmicTM

DNA extraction was carried out on 32 medicinal plant samples available in Malaysia using the TriOmicTM extraction kit. Amounts of 0.1 g flowers or young leaves were ground with liquid nitrogen, lysed at 65°C in RY1plus buffer and followed by RNAse treatment. Then, RY2 buffer was added to the samples and mixed completely by vortexing before removal of cell debris by ... more

A.R. Mohd-Hairul; A.B. Sade; B.C. Yiap; A.R. Raha

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