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Related GMR Articles

03/17/2014
Chinese muntjac; Microsatellite; Muntiacus reevesi; Polymorphism; Population genetics

Eight polymorphic microsatellite markers for Muntiacus reevesi were identified and characterized in this study. The number of alleles per locus ranged from 3 to 10 across 24-48 samples. The loci showed expected and observed heterozygosities of 0.577-0.876 and 0.387-0.933, respectively, with an average polymorphic information content value of 0.682. These markers should be a ... more

H. Wang; X. Luo; W.B. Shi; B.W. Zhang
12/19/2011
Cross-amplification; Lycoris radiata; Microsatellite; Molecular marker

Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs using a modified CTAB protocol. We isolated 10 ... more

S.Q. Xuan; J.Y. Zheng; H. Wang; P. Gao; S.B. Zhou; K. Liu; G.P. Zhu
08/16/2011
Harpadon nehereus; Microsatellite; Molecular marker

The Bombay duck, or bummalo (Harpadon nehereus), is a lizardfish native to the Arabian sea, but also common in the China sea. It is normally dried and salted before consumption and export. To provide molecular information on this economically important fish species, we developed and characterized microsatellite markers. Ninety positive clones from the (CA)15-enriched ... more

T.J. Xu; D.Q. Sun; H.Y. Li; R.X. Wang
09/12/2011
Genetic marker; Larus saundersi; Microsatellite; Polymorphism; Saunders’s gull

Nine polymorphic microsatellite loci were isolated, using tetranucleotide repeat oligonucleotide probes from an enriched DNA library of the globally “vulnerable” Saunders’s gull (Larus saundersi), collected from the Yancheng coastal wetland, one of the three remaining breeding sites in China. Six breast muscle tissues and 16 blood samples from 22 gulls and eight eggshell membrane ... more

H.X. Jiang; S.L. Ren; Y.Q. Hou; M. Yang; X.B. Wu
08/16/2011
Invasive species; Microsatellite; Polymorphism; Pseudorasbora parva

The Asiatic topmouth gudgeon, Pseudorasbora parva, is recognized as one of the most invasive fish species in many countries outside of Asia. We isolated and characterized 19 microsatellite loci from P. parva. The polymorphism of these 19 loci was tested on 40 individuals of P. parva sampled from a wild population located in Ezhou, Hubei province of China. The ... more

C. Zeng; Y. Gul; K. Yang; L. Cui; W.M. Wang; Z.X. Gao
07/25/2011
Coilia mystus; Conservation; Microsatellite; PIMA; RAPD-PCR enrichment

Coilia mystus is the most important harvested fish species in China; it inhabits quite different water environments during the different life history stages. Populations of C. mystus have dropped sharply due to overharvesting and water pollution. We developed eight microsatellite loci in C. mystus for conservation genetics studies. These new markers were tested ... more

J.Q. Yang; X.D. Zhou; D. Liu; Z.Z. Liu; W.Q. Tang
04/26/2011
Duchenne muscular dystrophy; Dystrophin gene; Han population genetics; Microsatellite; Polymorphism

To look for novel microsatellites in the dystrophin gene for the diagnosis of Duchenne muscular dystrophy, candidate microsatellite sites in the dystrophin gene were analyzed with the SSRHunter software and were also genotyped. Among the 15 candidate microsatellite sites, three novel microsatellite sites in the 60th, 30th, and 2nd intron were found to have a high degree of polymorphism. ... more

R.F. Sun; Y.S. Zhu; J.L. Feng; Z. Tian; W.J. Kuang; Y. Liu; H.B. Zhang; S.B. Li
02/08/2011
Microsatellite; Molecular marker; Scartelaos viridis

Scartelaos viridis (walking goby) is a small edible fish that inhabits warm inshore environments. To provide molecular information of S. viridis, we developed and characterized microsatellite markers for this species. Using (CA)15-enriched genomic libraries of Scartelaos viridis, 44 positive clones were sequenced; 34 sequences contained multiple repeat motifs ( ... more

D.Q. Sun; H.Y. Li; T.J. Xu; R.X. Wang
02/15/2011
Genetic marker; Microsatellite; Pelophylax hubeiensis; Polymorphism

Twelve polymorphic microsatellite loci were isolated from an (AC)n- and (AG)n-enriched DNA library for the endemic Chinese frog Pelophylax hubeiensis (Ranidae). The number of alleles per locus ranged from two to eight, with a mean of 5.17. The observed and expected heterozygosities ranged from 0.226 to 0.839 and from 0.204 to 0.826, with means of 0.568 and 0. ... more

J.C. Yan; F.H. Zhu; H.L. Wu
09/08/2010
Microsatellite; Molecular marker; Mugil cephalus

Using an (AG)13 enriched genomic library of Mugil cephalus, 12 polymorphic microsatellite loci were isolated and char­acterized in a test population; the number of alleles ranged from 2 to 11. The observed and expected heterozygosities ranged from 0.2593 to 0.8966 and from 0.3047 to 0.8454, respectively. Two loci deviated from Hardy-Weinberg equilibrium; linkage disequilibrium ... more

T.J. Xu; D.Q. Sun; G. Shi; R.X. Wang

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