Research Article

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Camellia sinensis; Expressed sequence tag; Haplotype; Nucleotide diversity; Single nucleotide polymorphisms

Tea is the second most popular non-alcoholic beverage in the world. In recent years, several molecular markers have been used in genetic studies of the tea plant. Yet, only a few single nucleotide polymorphisms (SNPs) have been reported. Here, we identified 818 putative SNPs from expressed sequence tag (EST) databases for the tea plant, which produced a frequency of 1 SNP/170 bp. A ... more

C.C. Zhang; L.Y. Wang; K. Wei; H. Cheng
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Dendrobium nobile; Expressed sequence tag; Simple sequence repeat marker

Expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers could enrich the current resource of molecular markers. In this study, microsatellite markers were developed for Dendrobium nobile Lindl. by mining the ESTs. Twenty-eight EST-SSRs amplified 2 to 6 nucleotide repeats with a mean number of 2.82 alleles per locus. The observed and expected heterozygosities per ... more

J.J. Lu; J.Y. Kang; S.R. Ye; H.Z. Wang
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Expressed sequence tag; Illumina sequencing; Odontobutis potamophila; Single nucleotide polymorphism validation

Transcriptome sequencing technology has been applied in the development and discovery of single nucleotide polymorphism (SNP) markers in fish. In this study, a panel of 120 expressed sequence tag (EST)-derived SNPs was selected by several selection filters from the resultant EST library of Odontobutis potamophila using Illumina Sequencing. In total, 37 SNPs from 120 putative SNPs ... more

H.W. Zhang; S.W. Yin; L.J. Zhang; X.Y. Hou; Y.Y. Wang; G.S. Zhang
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Aspergillus; Coffea arabica; Ochratoxin

Ten fungal isolates from coffee beans were morphologi­cally identified as Aspergillus niger, A. ochraceus and A. carbonari­us (N = 5, 3, and 2, respectively). Only one isolate, morphologically identified as A. niger, was unable to produce ochratoxin A (OTA). This may be a new species in the Aspergillus section Nigri. OTA levels in all ... more

M.A. Moslem; A. Mashraqi; K.A. Abd-Elsalam; A.H. Bahkali; M.A. Elnagaer
12/02/2016
Alcohol Oxidoreductases; Anthocyanins; Cloning, Molecular; Flavonols; Fruit; Gene Expression Regulation, Plant; Glucosyltransferases; Organ Specificity; Plant Proteins; Transcription factors; Vitis

MYBA2 transcription factor (Myb-related gene) affects the coloring in grapevine berry and plays an important role in the biosynthesis of anthocyanin. The MYBA2 gene was cloned from Vitis vinifera L. cv. Cabernet Sauvignon and polyclonal antibodies for VvmybA2 were prepared. The VvmybA2 gene expression patterns were observed in seven tissues (the leaf, stem, flower, bud, root, berry, and ... more

T.Q. Niu; Z.D. Gao; P.F. Zhang; X.J. Zhang; M.Y. Gao; W. Ji; W.X. Fan; P.F. Wen
12/19/2016
Animals; Antimutagenic Agents; Antioxidants; Chromosome aberrations; Cyclophosphamide; Fruit; male; Malpighiaceae; Models, Animal; Plant Extracts; Rats; Rats, Wistar

Barbados cherry (BC) (Malpighia glabra Linnaeus) is a functional fruit that is consumed to prevent disease. It is used as an adjuvant in the treatment of several diseases, and acts as an antianemic, an appetite stimulant, a wound healer, an anti-inflammatory, a mineralizer, an antifungal, and an antioxidant agent. Several chemotherapeutic agents, such as cyclophosphamide, may result in ... more

E. Düsman; I.V. Almeida; L.T.D. Tonin; V.E.P. Vicentini
12/23/2016
Fruit; Gene expression profiling; Gene Expression Regulation, Plant; Mutation; Prunus persica; Transcriptome

Fruit ripening is a complex developmental process, the details of which remain largely unknown in fleshy fruits. In this paper, the fruit flesh of two peach varieties, "Zhongyou9" (a nectarine; Prunus persica L. Batsch) and its mutant "Hongyu", was analyzed by RNA-seq technology during two stages of ripening at 20-day intervals. One hundred and eighty significant upregulated and two hundred ... more

H.F. Pan; Y. Sheng; Z.H. Gao; H.L. Chen; Y.J. Qi; X.K. Yi; G.H. Qin; J.Y. Zhang
12/23/2016
Fruit; Gibberellins; Organophosphorus Compounds; Photosynthesis; Plant Growth Regulators; Plant Leaves; Pyrus

To identify the roles of ethylene in fruit development in Japanese pear Pyrus pyrifolia 'Niitaka', one of the non-climacteric genotypes, source-sink strength and fruit development during fruit expansion were investigated when ethephon was applied after a conventional gibberellic acid (GA) lanolin paste treatment on the pedicel. The results demonstrate that the conventional GA treatment during ... more

C. Ma; L. Wang; U.Y. Lee; K. Tanabe; S. Kang; C.X. Zhang
2/08/2017
Fruit; Meliaceae; Plant Extracts; Plant Proteins; Proteomics

We investigated the extraction of Toona sinensis fruit proteins and preliminarily characterized their physicochemical properties. The results showed that optimal extraction occurred under conditions of pH 10.5, a duration of 40 min, a liquid-to-solid ratio of 25:1, and a temperature of 40°C by an orthogonal design using T. sinensis fruit protein as the index and single factor. The total ... more

W.Z. Li; W.N. Han; B. Liu; S.H. Ding; X.K. Zhang; R.S. Wang
2/23/2017
Ascomycota; Brazil; Carica; Crops, Agricultural; Disease resistance; Fruit; Genotype; Hybrid Vigor; Hybridization, Genetic; Inbreeding; Plant breeding; Plant Diseases; Plant Leaves; Reproducibility of Results

Papaya crop is important to Brazilian agribusiness. However, the expansion of papaya cultivation in the country is affected by the absence of commercial cultivars presenting good disease resistance. The black-spot caused by the fungus Asperisporium caricae is the most damaging foliar disease affecting Brazilian papaya crops. The use of genetically resistant cultivars is a promising strategy to ... more

T.P.S. Poltronieri; S.F. Silveira; M. Vivas; S. Catarina; D.F.M. Cortes; A.O.N. Azevedo; M.G. Pereira

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