Research Article

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Besides the host immune response, genetic and environmental factors play crucial roles in the manifestation of hepatitis B virus (HBV) infection. “Regulated on activation normal T-cell expressed and secreted” factor (RANTES) plays a vital role in CD4+, CD8+ T-lymphocyte and dendritic cell activation and proliferation in inflammation. Single nucleotide polymorphisms ... more

A. Al-Qahtani; S. Alarifi; M. Al-Okail; Z. Hussain; A. Abdo; F. Sanai; M. Al-Anazi; N. Khalaf; H. Al-Humaidan; M. Al-Ahdal; F.N. Almajhdi
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Hepatitis B virus (HBV) is the infectious agent of both acute and chronic hepatitis. HBV exists in multiple genotypic variants that differ in their capacity to become persistent chronic infections and in their clinical manifestations, including hepatocellular carcinoma. The 8 genotypes (A-H) of HBV show a specific worldwide geographic distribution and are correlated with different disease ... more

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Penicillium griseoroseum, a deuteromycete fungus producer of pectinolytic enzymes, was transformed with a gene encoding for green fluorescent protein (GFP). The selection of transformants was based on the homologous nitrate reductase gene (niaD). Protoplasts of a P. griseoroseum Nia mutant (PG63) were co-transformed with the plasmids pNPG1 and pAN52-1-GFP. ... more

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Mutant; ROP signaling

The importance of the ROP small GTPase signaling pathway in the regulation of cellular polarity growth in eukaryotes has been thoroughly studied. In this study, we examined the LeROP small GTPase (related to Arabidopsis thaliana genome LeROP GTPase in tomato) signaling of cell polarity growth in the mutant (M-1) tomato. Interestingly, we detected expansive growth of epidermis ... more

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Hepatitis B virus; HepG2 cell; Hygromycin; Stable cell line

HepG2.2.15 cell is a widely used cell model for studying HBV (hepatitis B virus) in vitro. In these cells, the HBV genome is integrated in several sites of HepG2 cellular DNA. These multiple copies may have some influence on the cellular processes. We constructed a new plasmid, pSEH-Flag-HBV, and transfected it into HepG2 cells, and then screened it with hygromycin. We then used ... more

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An association between the sequence variants of cytokine genes and various clinical outcomes in subjects infected with the hepatitis B virus (HBV) has been demonstrated. However, the results are inconsistent and inconclusive. Further studies in other populations and the evaluation of a greater number of individuals may contribute to a better understanding of the influence of the cytokine ... more

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The aim of this study was to investigate the effects of inhibition of the hepatitis B virus (HBV) S gene by polypurine region locked nucleic acid on viral replication in cells. We designed and synthe­sized a locked nucleic acid, phosphorothioate oligonucleotides, unmod­ified oligonucleotides, and unrelated control sequence for the hepatitis B virus S gene polypurine ... more

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L. Liu; Y. Yan; J. Zhou; L.W. Huang; C.P. He; K. Ling; H.C. Zhou; Q.M. Wen; X.M. Wang