Research Article

Related GMR Articles

Bioinformatics; Brain; GPCR; Orphan receptor; Prostate cancer; Testis

G protein-coupled receptors (GPCRs) are involved in a large variety of physiological functions. The number of known members that belong to this large family of receptors has been rapidly increasing. Now, with the availability of the human genome sequence databases, further family members are being identified. We describe the identification of a novel GPCR that shows no significant ... more

R.B. Parmigiani; G.S. Magalhães; P.A.F. Galante; C.V.B. Manzini; A.A. Camargo; B. Malnic
Brain; Contrast-enhanced ultrasound; Conventional ultrasound; Crossbreed dogs; Gunshot wound

This study aimed to investigate the characteristic features of craniocerebral gunshot wounds by conventional ultrasound (CUS) and evaluate the efficacy of contrast-enhanced ultrasound (CEUS) in differentiation of tissue condition in wounds. Twenty crossbreed dogs (treatment: N = 15; control: N = 5) were used in the study. Pipe-shaped hyperechoes of varying size were found by CUS in most ... more

D. Deng; G. Dan; J. Tao; X.B. Wu; Z. Chen; M. Chang; M.S. Liao; F. He
Animal model; Cyclin D1; Granulocyte macrophage colony generation; Jianpi Bushen Prescription; Radiation damage; Wnt3a

The effects of the traditional Chinese drug Jianpi Bushen Prescription (JBP) were investigated on expressions of Wnt3a and Cyclin D1 genes in radiation-damaged mice. The radiation damage model was induced in Kumming mice by single total body irradiation treatment for 9 days. Mice were divided into the radiation group, low-dose (100%) JBP group, high-dose (200%) JBP group ... more

D.C. He; J.J. Xiao; Y. Zhang; H. Lin; X.J. Ding; Y. Tu
Animal model; Cerebellum; FMR1; FXTAS; Microarrays

Modifications in the GABA pathway are considered to be responsible for motor alterations in animal models for fragile X-associated tremor ataxia syndrome. We analyzed the expression profile in the cerebellum in a transgenic mouse model that over expresses the human FMR1 gene with CGG repeats in the normal range. We used the “GeneChip Mouse Gene 1.0 ST Array” from Affymetrix ... more

J.J. Fernández; R. Martínez; E. Andújar; M. Pérez-Alegre; A. Costa; V. Bonilla-Henao; F. Sobrino; C.Ó. Pintado; E. Pintado
Animal model; Bax; Bcl-2 gene; Chemical damage; Jianpi Bushen Prescription; SHP-1

We investigated the effect of Jianpi Bushen prescription (JBP) on the expression of the SHP-1 and apoptosis-related genes in chemically damaged model mice and a compound e-jiao slurry (EJS) group (positive control). Kunming mice received an abdominal injection of 100 mg/kg cyclophosphamide once a day for 3 consecutive days to induce chemical damage. The mice underwent lavage at a ... more

D.C. He; J.J. Xiao; Y. Zhang; H. Lin; X.J. Ding; Y. Tu
2017 May 10
Animals; Brain; Cloning, Molecular; Fish Proteins; Fishes; Kidney; Liver; Muscle, Skeletal; Receptors, Glucocorticoid; Sodium Chloride; Stress, Physiological

To understand the regulation of the glucocorticoid receptor gene (Gcr) during loading and transport stress in fish, the Gcr gene of Coilia nasus was cloned. Gcr in C. nasus is expressed strongly in the liver and muscle, and less stronglyin the gills, brain, spleen, intestine, trunk kidney, and head kidney. Gcr expression in both the liver and muscle was increased by loading and transport ... more

F.K. Du; G.C. Xu; Y. Li; Z.J. Nie; P. Xu
2017 Sep 27
Animals; Anovulation; Brain; Female; Interleukin-6; Leptin; Lipid metabolism; Mice; Mice, Obese; Receptor Protein-Tyrosine Kinases; Receptors, GABA; Receptors, Muscarinic; Receptors, Serotonin; Synaptic Transmission

Polycystic ovary syndrome is the classic example of loss of functional cyclicity and anomalous feedback. In this case, the excessive extra-glandular production and conversion of androgens to estrogens are the pathophysiological basis of the chronic anovulation. The literature describes an experimental model of the polymicrocystic ovary in obese diabetic mice with insulin resistance. The fact ... more

M.A. Azevedo; I.D.C.G. Silva
Ethanol; PVP; RNA isolation; RT-PCR; Sunflower seeds; β-mercaptoethanol

In an attempt to isolate high-quality, intact total RNA from sunflower (Helianthus annuus) seeds for investigation of the molecular mechanisms of mutations, we tested various procedures, using kits, including RNAiso Plus, RNAiso Plus+RNAiso-mate for Plant Tissue, Trizol, and the Qi method, but no high-quality total RNA of high integrity was obtained with any of these methods, ... more

J. Yang; X.B. Ma
Complementarity method; Ethanol; Genotype x environment interaction; Multi-environment trial; Sorghum bicolor

Sweet sorghum has considerable potential for ethanol and energy production. The crop is adaptable and can be grown under a wide range of cultivation conditions in marginal areas; however, studies of phenotypic stability are lacking under tropical conditions. Various methods can be used to assess the stability of the crop. Some of these methods generate the same basic information, whereas ... more

U.J. de Figueiredo; J.A.R. Nunes; R.Ada C. Parrella; E.D. Souza; A.R. da Silva; B.M. Emygdio; J.R.A. Machado; F.D. Tardin
2017 Mar 16
Animals; Aquaculture; Cichlids; Ethanol; Fish Proteins; Gene Expression Regulation; male; Muscle, Skeletal; Myostatin; Organ Size; Plant Extracts; Propolis

Propolis can be used as growth enhancer due to its antimicrobial, antioxidant, and immune-stimulant properties, but its effects on morphometry and muscle gene expression are largely unknown. The present study evaluates the influence of propolis on muscle morphometry and myostatin gene expression in Nile tilapia (Oreochromis niloticus) bred in net cages. Reversed males (GIFT strain) with an ... more

E.L. Buck; I.Y. Mizubuti; A.A. Alfieri; R.A.A. Otonel; L.Y. Buck; F.P. Souza; O.P. Prado-Calixto; A.R. Poveda-Parra; A. Filho; N.M. Lopera-Barrero