Research Article

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12/19/2014
Adipocyte nuclei; Cryopreservation; Dimethyl sulfoxide; DNA nuclear integrity; Flow cytometry; Isolation

Cryopreservation injuries involve nuclear DNA damage. A protocol for cryopreserving and isolating adipocyte nuclei is proposed. Adipose tissue samples were directly analyzed (NoCRYO-0h), or stored at -196°C for 7 days without 10% dimethyl sulfoxide (DMSO) (CRYO-WO-DMSO) or with DMSO (CRYO-W-DMSO). To determine the effect of DMSO on cryopreservation treatment, adipose tissue samples were ... more

M.M. Almeida; L.C.J. Caires; C.M. Musso; J.M.S. Campos; C.M.C. Maranduba; G.C. Macedo; J.P.R.F. Mendonça; R.M.G. Garcia
05/15/2012
NANOG; Neural differentiation; NT2/D1; OCT4; Proliferation; SOX2

SOX2, a universal marker of pluripotent stem cells, is a transcription factor that helps control embryonic development in vertebrates; its expression persists in neural stem/progenitor cells into adulthood. Considering the critical role of the SOX2 transcription factor in the regulation of genes required for self-renewal and pluripotency of stem cells, we developed and ... more

D. Drakulic; A. Krstic; M. Stevanovic
07/15/2013
Nanog3; Neural tube defects; OCT4; SOX2; Stem cell

Anencephaly and myelomeningocele are the 2 most common forms of neural tube defects (NTDs). During embryogenesis large numbers of extrinsic and intrinsic factors are responsible for the closing of the neural tube. "Stem cells" maintain the pluripotency during differentiation of 3 germ layers, including the neural ectoderm. We examined the role of Oct4, Nanog3, and Sox2 genes in the ... more

A.K. Saxena; S. Pandey; L.K. Pandey
08/04/2009
Amniotic fluid; Human phosphate binding protein; Proteomics

Human amniotic fluid is of both maternal and fetal origin; it protects the fetus and provides the environment for growth and development of the fetus. We used a proteomics-based approach for targeting and purifying human phosphate binding protein, a member of the DING family of proteins from amniotic fluid, using Blue Sepharose CL-6B, DEAE-Sephacel and gel filtration chroma­tography. The ... more

M. Alam; M. Mahajan; M. Raziuddin; T.P. Singh; S. Yadav
12/19/2016
Amniotic fluid; Chromosomes, Human; Chromosomes, Human, Pair 13; Chromosomes, Human, Pair 18; Chromosomes, Human, Pair 21; Chromosomes, Human, X; Chromosomes, Human, Y; Female; Gene frequency; Genetic variation; Humans; Iran; male; Microsatellite Repeats; Pregnancy; Prenatal diagnosis; White People

Quantitative fluorescent polymerase chain reaction (QF-PCR), in recent years, has been accepted as a rapid, high throughput, and sensitive method for prenatal diagnosis of common chromosomal aneuploidies. Since short tandem repeats (STRs) are the cornerstone of QF-PCR technique, selection of the most polymorphic STR markers is an essential step for a successful QF-PCR assay. The genetic ... more

J. Saberzadeh; M.R. Miri; M.B. Tabei; M. Dianatpour; M. Fardaei
12/13/2013
Aquaporins; Cryopreservation; Cryoprotective agents; Intracellular ice formation; Preantral follicles

The discovery of water channels (aquaporins, AQPs) was a landmark event for the clarification of water transport through the plasma membrane. AQPs belong to a family of intrinsic membrane proteins that act as selective channels for water and for solutes such as glycerol and urea. AQPs were found in different tissues and organs, including male and female reproductive systems. In the swine ... more

A.D. Sales; C.H. Lobo; A.A. Carvalho; A.A. Moura; A.P.R. Rodrigues
12/19/2016
Cell survival; Cryopreservation; Cryoprotective agents; Desiccation; Polyploidy; Regeneration; Schisandra

Cryopreservation has been proven significance as a technique for promising the long-term conservation of plant germplasms. This study aimed to establish a cryopreservation protocol for calli of Schisandra chinensis (Turcz.) Baill, and to explore the effects of different process parameters on callus viability. Effects of desiccation duration, cryoprotectants and cryopreservation methods, ... more

D. Sun; Y.F. Yu; H.Y. Qin; P.L. Xu; Y. Zhao; Y.X. Liu; Z.X. Wang; S.T. Fan; Y.M. Yang; J. Ai
3/22/2017
Animals; Cryopreservation; Disease Models, Animal; Glial cell line-derived neurotrophic factor; Interleukin-10; male; Mesenchymal Stem Cell Transplantation; Mesenchymal stem cells; Neuroprotective Agents; Rats; Rats, Inbred Lew; Regenerative medicine; Spinal Cord Injuries; Transforming Growth Factor beta; Tumor necrosis factor-alpha; Vascular Endothelial Growth Factor A

This study aimed to evaluate the immunomodulatory and neuroprotective effects of allogeneic and cryopreserved mesenchymal stem cells (MSCs) on spinal cord injury. A total of 120 rats were distributed into the following groups: negative control (NC) - without injury, positive control (PC) - with injury without treatment, and group treated with MSC (GMSC) - with injury and treated. Motor ... more

I.R. Rosado; P.H. Carvalho; E.G.L. Alves; T.M. Tagushi; J.L. Carvalho; J.F. Silva; M.S.L. Lavor; K.M. Oliveira; R. Serakides; A.M. Goes; E.G. Melo
07/08/2013
Duck; Duplex-specific nuclease; Embryo development; NANOG; Subtractive hybridization

Several studies have documented the process of early embryonic development in poultry; however, the molecular mechanisms underlying its developmental regulation are poorly understood, particularly in ducks. In this study, we analyzed differential gene expression of embryos 6 and 25 h following oviposition to determine which genes regulate the early developmental stage in ducks. Among 216 ... more

Y.L. Liu; L.X. Zhong; J.J. Li; J.D. Shen; D.Q. Wang; Z.R. Tao; F.X. Shi; L.Z. Lu
06/26/2015
Differentiation; Erythropoietin; Mesenchymal stem cell; Proliferation; Signaling pathways

We examined whether erythropoietin (EPO) can inhibit adipogenic differentiation of mesenchymal stem cells (MSCs) in the mouse bone marrow and its underlying mechanism. We separated and extracted mouse bone marrow MSCs and induced adipogenic differen­tiation using 3-isobutyl-1-methylxanthine, insulin, and dexamethasone. Different concentrations of EPO were added to the cells and observed ... more

G.X. Liu; J.C. Zhu; X.Y. Chen; A.Z. Zhu; C.C. Liu; Q. Lai; S.T. Chen

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