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AFLP; Alfalfa; Common leafspot; Resistance; SCAR

To identify amplified fragment length polymorphism (AFLP) markers associated with resistance or susceptibility of alfalfa to common leafspot (CLS) caused by the fungus Pseudopeziza medicaginis (Dermateaceae), bulked segregant analysis was conducted based on an F1(M × M) population of 93 plants and a BC1S population of 91 plants. Three AFLP markers, ACTCAA ... more

Y. Wang; B. Bi; Q.H. Yuan; X.L. Li; J.M. Gao
AFLP; Aquilegia; Columbine; Genetic diversity

Species of the genus Aquilegia are exceptionally diverse in their floral morphology and color, commonly known as columbine. They are widely planted ornamentals and are highly attractive for hummingbirds. However, little is known about their genetic diversity. We examined the genetic diversity of the species and cultivars using amplified fragment length polymorphism (AFLP) ... more

R.R. Zhu; Y.K. Gao; L.J. Xu; Q.X. Zhang
Adulteration; AFLP; DNA fingerprinting; Molecular marker; Zingiber spp

The Zingiber genus, which includes the herbs known as gingers, commonly used in cooking, is well known for its medicinal properties, as described in the Indian pharmacopoeia. Different members of this genus, although somewhat similar in morphology, differ widely in their pharmacological and therapeutic properties. The most important species of this genus, with maximal therapeutic ... more

S. Ghosh; P.B. Majumder; S. Mandi
AFLP; Amazon region; Anopheles darlingi; Genetic variability; Malaria vector; Population differences

Anopheles darlingi is the most important malaria vector in Central and South America. After a dramatic reduction of malaria cases in the whole Brazilian territory, with the lowest abundance being attained by 1970, the disease resurged in the Amazon region, where it is now a great public health concern. Consequently, better knowledge about vector species became urgent. We ... more

L.M. Santos; R.A. Gama; A.E. Eiras; C.G. Fonseca
Abalone; AFLP; Domestication; Genetic diversity; SSCP; Stock identification

Amplified fragment length polymorphism (AFLP) analysis was carried out on representative individuals of wild Haliotis asinina using 64 primer combinations. Nine polymorphic AFLPs were cloned and sequenced. Sequence-specific primers were designed from six AFLP-derived fragments. Three sequence-characterized amplified region (SCAR) markers (HaSCAR320, HaSCAR295 ... more

P. Praipue; S. Klinbunga; P. Jarayabhand
AFLP; Population differentiation; Species-specific marker; SSCP; Stingless bees; Trigona collina

A molecular maker for authenticating species origin of the stingless bee (Trigona collina) was developed. Initially, amplified fragment length polymorphism analysis was made of 11 stingless bee species using 64 primer combinations. A 316-bp band found only in T. collina was cloned and sequenced. A primer pair (CUTc1-F/R) was designed and tested for species-specificity in ... more

M. Theeraapisakkun; S. Klinbunga; S. Sittipraneed
AFLP; Grapevine rootstock; Molecular analysis

Amplified fragment length polymorphism (AFLP) technique was used to assess genetic relationships among 20 grapevine rootstocks in Turkey. Discrimination of the rootstocks with 10 primer combinations yielded 1366 bands on AFLP gels; 65% of them were polymorphic. The rootstocks revealed two main clusters; one of them comprised two (Malégue and Harmony) and the other 18 genotypes. The ... more

A. Ergül; S. Aras; G. Söylemezoğlu
AFLP; Cytoplasmic male sterility; Molecular marker; mtDNA; Wheat

To produce a good F1 hybrid variety wheat crop, it is necessary to explore novel cytoplasmic male sterility (CMS) lines and their maintainer line. This study aimed to identify cytoplasmic variation in three isonuclear-alloplasmic male sterile lines Aegilops kotschyi (Ae.kots) -90-110, Aegilops ventricosa (Ae.ven) -90-110, and Triticum spelta ( ... more

M. Ejaz; Z. Qidi; Z. Gaisheng; W. Qunzhu; Z. Xinbo
AFLP; Genetic diversity; Procambarus clarkii; Yangtze River

The red swamp crayfish has become one of the most important freshwater aquaculture species in China. At present, although it is widely distributed in the middle and lower reaches of the Yangze River basin, little is known about its population genetics and geographic distribution in China. We estimated the genetic diversity among 6 crayfish populations from 4 lakes (Hongze Lake, Poyang ... more

B.F. Zhu; Y. Huang; Y.G. Dai; C.W. Bi; C.Y. Hu
AFLP; Colombia; Genetic diversity; Heliconia; Phylogenetic relationships

Researchers have classified the Heliconia genus as a group of highly variable and diverse plants. Species and cultivars are visually differentiated primarily on the basis of the color and size of inflorescence bracts. At taxonomic level, flower type (parabolic, sigmoid, or erect) and size are taken into account. The vast morphological diversity of heliconias at intra-specific, ... more

L. Isaza; M.L. Marulanda; A.M. López