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Related GMR Articles

Alternative splicing variant; DRD2 gene; Expression; Pig; Polymorphism

The dopamine D2 receptor (DRD2) is a crucial mediator for normal physiological processes. We cloned the pig DRD2 gene, investigated its distribution in tissues and identified polymorphisms by RT-PCR, quantitative real-time PCR and direct sequencing. Two Yorkshire pigs from Guangdong Academy of Agricultural Sciences (Guangzhou, China) were selected to clone the gene and ... more

H.P. Xu; X.M. He; M.X. Fang; Y.S. Hu; X.Z. Jia; Q.H. Nie; X.Q. Zhang
Escherichia coli F18; Pig; Real-time PCR; TLR4 gene

TLR4 is the main recognition receptor of bacterial lipopolysaccharides, which play an important role in innate and adaptive immunity. We used real-time PCR to analyze the tissue expression profile and differential expression of TLR4 in 4 pig populations (Escherichia coli F18-resistant Sutai, E. coli F18-sensitive Sutai, Large White, Meishan), in order ... more

J. Wang; Z.Y. Pan; X.R. Zheng; Z.C. Wu; X.M. Su; G.Q. Zhu; X.G. Huang; S.L. Wu; W.B. Bao
BPI gene; Gene expression; Pig; Real-time PCR

We compared and analyzed the expression of the BPI gene of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 by the real-time PCR method, in order to determine if it is involved in protection against disease caused by ETEC F18. There was a significant difference between 18 and 35-day expres­sion in the jejunum. There were also significant differences ... more

J. Zhu; C. Zi; Z.C. Wu; L. Liu; X.R. Zheng; X.M. Su; G.Q. Zhu; X.G. Huang; S.L. Wu; W.B. Bao
Milk; miRNA; Normalization; Pig; Quantitative PCR

Determination of an optimal set/number of internal control microRNA (miRNA) genes is a critical, but often undervalued, detail of quantitative gene expression analysis. No validated internal genes for miRNA quantitative PCR (q-PCR) in pig milk were available. We compared the expression stability of six porcine internal control miRNA genes in pig milk from different lactation periods (1 h ... more

Y.R. Gu; Y. Liang; J.J. Gong; K. Zeng; Z.Q. Li; Y.F. Lei; Z.P. He; X.B. Lv
ACSL4; Backfat thickness; H-FABP; Intramuscular fat content; Pig; Polymorphism

Here, we analyzed the distribution of H-FABP/(HinfI, MspI, and HaeIII) and ACSL4/RsaI polymorphisms, and the associations of these 4 polymorphic loci with intramuscular fat (IMF) content and backfat thickness (BFT) in Yanan, Jinhua, Duroc, Landrace, Yorkshire, and Duroc x (Landrace x Yorkshire) (DLY) pigs. H-FABP/Hin ... more

J.N. Chen; Y.Z. Jiang; W.M. Cen; S.H. Xing; L. Zhu; G.Q. Tang; M.Z. Li; A.A. Jiang; P.E. Lou; A.X. Wen; Q. Wang; T. He; G.X. Zhu; M. Xie; X.W. Li
Escherichia coli F18; Pig; Real-time polymerase chain reaction; TAP1 gene

The transporter associated with antigen processing (TAP) transports peptides from the cytosol into the endoplasmic reticulum for subsequent loading onto the major histocompatibility complex (MHC) class I molecules. This study showed the dynamic changes in the TAP1 expression level in newborn to weaning piglets. Tissue expression profiles revealed that the TAP1 gene was ... more

J. Wang; Y. Liu; W.H. Dong; Y.J. Huo; X.G. Huang; S.L. Wu; W.B. Bao
Expression patterns; Pig; Promoter activity; UGP2

The function of the UDP-glucose pyrophosphorylase 2 gene (UGP2) in pig is not clear. In the present study, we used RNA isolated from Large White pigs and Chinese indigenous MeiShan pigs to examine the temporal coordination of changes in gene expression within muscle tissues. We cloned both the complete genomic DNA sequence and 2077-bp 5ꞌ-flanking sequence of porcine ... more

J.Y. Yu; S.M. Shao; K. Chen; M.G. Lei; Y.Z. Xiong
Gene function; PGM1; Pig; Promoter region; Skeletal muscle

The phosphoglucomutase 1 (PGM1) gene was differentially expressed in tissues of Chinese Meishan and Large White pigs. In this study, the promoter region, expression profile, and genetic mutations of the gene were determined. Expression of a 5'-deletion in both C2C12 and PK-15 cells showed that a negative regulatory element was at -1871 to +185 bp and a positive regulatory ... more

J.Y. Yu; S.M. Shao; Y.Z. Xiong
Developmental expression; Glucose transporter; Longissimus dorsi muscle; Pig; Quantitative real-time PCR

Glucose transporter proteins 2 and 4 (GLUT2 and GLUT4) play important roles in glucose transport and energy metabolism. Changes in the levels of GLUT2 and GLUT4 mRNA were measured in longissimus dorsi muscle from the lean Yorkshire and fat Tibetan pig breeds at six different time points (1, 2, 3, 4, 5, and 6 months) with quantitative real-time polymerase chain reactions. ... more

Y. Liang; X.M. Yang; Y.R. Gu; X. Tao; Z.Z. Zhong; J.J. Gong; X.H. Chen; X.B. Lv
Gene expression analysis; Id1; mRNA; PBMCs; Proliferation; Psoriasis

Although the precise causes of psoriasis are unclear, it is widely accepted that psoriasis is a disorder in which factors in the immune system, enzymes, and other biochemical substances that regulate skin cell division are impaired, leading to rapid proliferation of keratinocytes and incomplete keratinization. Expression of the helix-loop-helix transcription factor Id1 (inhibitor of ... more

C. Ronpirin; M. Achariyakul; T. Tencomnao; J. Wongpiyabovorn; W. Chaicumpa