Research Article

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05/11/2007
Amino acids; Cassava; Interspecific hybrids; Lysine; Methionine; Protein content

Cassava roots have a low-protein content (0.7-2%). Amino acids such as lysine and methionine are also low, and some research reports have indicated the absence of methionine. The amino acid profiles of a common cassava cultivar and an interspecific hybrid, namely ICB 300, were determined using the computerized amino acid analyzer Hitachi L-8500. The interspecific hybrid has 10 times ... more

N.M.A. Nassar; M.V. Sousa
11/11/2006
Cassava; India; Plant breeding; Productivity

South America is responsible for about half of the cassava world production. In the 1970’s productivity of the crop on the continent was about 15 ton/ha, and dropped continuously until reaching 12 ton/ha in 2004. India’s productivity of cassava increased from 10 ton/ha in the 1970’s to 28 ton/ha in 2004. Brazil contributed significantly to improving cassava crops through the Instituto ... more

N.M.A. Nassar
03/24/2009
Cassava; In silico analysis; Inverse polymerase chain reaction; Pt2L4 glutamic acid-rich protein; Root promoter; Transient expression

Cassava is one of the most important tropical food crops for more than 600 million people worldwide. Transgenic technologies can be useful for increasing its nutritional value and its resistance to viral diseases and insect pests. However, tissue-specific promoters that guarantee correct expression of transgenes would be necessary. We used inverse polymerase chain reaction to isolate a ... more

C.R. de Souza; F.J. Aragão; E.C.O. Moreira; C.N.M. Costa; S.B. Nascimento; L.J. Carvalho
06/07/2011
Cassava; Gene expression; Gene walking; promoters

Cassava can be cultivated on impoverished soils with minimum inputs, and its storage roots are a staple food for millions in Africa. However, these roots are low in bioavailable nutrients and in protein content, contain cyanogenic glycosides, and suffer from a very short post-harvest shelf-life, and the plant is susceptible to viral and bacterial diseases prevalent in Africa. The demand ... more

M.A. Gbadegesin; J.R. Beeching
04/12/2013
Cassava; Essential amino acids; Interspecific hybrids; Protein

Cassava (Manihot esculenta) is a principal food for large populations of poor people in the tropics and subtropics. Its edible roots are poor in protein and lack several essential amino acids. Interspecific hybrids may acquire high protein characteristics from wild species. We analyzed 19 hybrids of M. esculenta with its wild relative, M. oligantha, for crude ... more

P.T.C. Gomes; N.M.A. Nassar
09/30/2007
genetic relationships; Groundnut; Microsatellites; peanut; Simple sequence repeat

The Kayabi Indians who inhabit the Xingu Indigenous Park, located in West Central Brazil, have grown and managed peanuts for a long time. A great number of landraces are being maintained by these tribes and some of this germplasm has morphological traits that exceed the variation described in the taxonomic literature. Here, we analyzed the genetic variability of these landraces using a ... more

F.O. Freitas; M.C. Moretzsohn; J.F.M. Valls
10/05/2007
FISH; Genomic library; Jackknife estimate; Microsatellites; Piracema species; Prochilodus lineatus

We described five novel microsatellite loci for the piracema fish species Prochilodus lineatus (Characiformes), endemic to South America and of extreme importance to both commercial and artisanal fisheries across its occurrence area. A primary, unenriched genomic library was constructed and radioactively screened for repetitive motifs. Positive clones were automatically ... more

G.M. Yazbeck; E. Kalapothakis
05/22/2007
Blastocerus dichotomus; Cervidae; Marsh deer; Microsatellites; Transferability

Blastocerus dichotomus, the marsh deer, is the largest Brazilian Cervidae species. The species is endangered because of hunting and loss of its natural habitat, i.e., flood plain areas, because of hydroelectric power station construction and agricultural land expansion. In the present study, we tested 38 microsatellite loci from four Cervidae species: Odocoileus ... more

K.C.E. Leite; R.G. Collevatti; T.R. Menegasso; W.M. Tomas; J.M.B. Duarte
05/31/2007
Genetic variability; Microsatellites; Nuclear molecular markers; Population structure; Wading bird

Roseate Spoonbills (Platalea ajaja, Linnaeus) are wading birds present in two of the most important Brazilian wetlands: the Pantanal wetlands and Rio Grande do Sul marshes. Natural populations of these species have not been previously studied with variable nuclear molecular markers. In order to support decision making regarding the management and conservation of these populations, we ... more

C.I. Miño; S.N. Del Lama
12/05/2006
CG content; Hyperthermophiles; Microsatellites; Optimum growth temperature; SSRs; Thermophiles

All organisms that have been studied until now have been found to have differential distribution of simple sequence repeats (SSRs), with more SSRs in intergenic than in coding sequences. SSR distribution was investigated in Archaea genomes where complete chromosome sequences of 19 Archaea were analyzed with the program SPUTNIK to find di- to penta-nucleotide repeats. The number of ... more

S. Trivedi

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