Research Article

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DNA sequence; Gene-rich region; Molecular marker; Resistance; Yellow rust

Analysis of DNA sequence variation among genotypes is useful for differentiation of wheat accessions, selection strategies and genetic development of crop plants. We screened molecular markers for yellow rust resistance genes (Yr7, Yr9, Yr15, Yr18, Yr26, and YrH52), which are in the gene-rich regions of wheat chromosomes 1B, 2B, and 7D ... more

E. Cabuk; Y. Aydin; A.A. Uncuoglu
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Average and variance; DNA sequence; Graphical representation; Invariant; Sequence comparison

li,j denotes the distance between the point (xi, yi) and the point (xj, yi) in graphical representation. By classifying li,j, i, j = 1, 2,…, N according to the number of points between (xi, yi) and (x ... more

Y.W. Liu; Y. Peng
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Calpastatin; Cyprinus carpio; Real-time PCR; Tissue expression

Calpastatin, an important protein used to regulate meat quality traits in animals, is encoded by the CAST gene. The aim of the present study was to clone the cDNA sequence of the CAST gene and detect the expression of CAST in the tissues of Cyprinus carpio. The cDNA of the C. carpio CAST gene, amplified using rapid amplification of cDNA ends ... more

W.X. Chen; Y. Ma
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Brangus; Carcass; DNA; Growth; Growth hormone; SNP

Sequence polymorphisms in the growth hormone (GH) gene and its transcriptional regulators, Pit-1 and Prop-1, were evaluated for associations with growth and carcass traits in two populations of Brangus bulls Chihuahuan Desert Rangeland Research Center (CDRRC, N = 248 from 14 sires) and a cooperating breeding program (COOP, N = 186 from 34 sires). Polymorphisms were SNP mutations in intron 4 (C ... more

M.G. Thomas; R.M. Enns; K.L. Shirley; M.D. Garcia; A.J. Garrett; G.A. Silver
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Dog breeds; Growth; Polymorphism; SNP; SOCS2

Suppressor of cytokine signaling 2 (SOCS2) is a negative regulator of growth hormone signaling. The deletion of SOCS2 in mice results in a 30-50% increase in post-natal growth. In an effort to identify polymorphisms in the SOCS2 gene that may be associated with body size in dogs, we characterized the canine SOCS2 gene and analyzed its genetic diversity among small and large dog breeds ... more

G. Rincón; A.E. Young; D.L. Bannasch; J.F. Medrano
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Bos taurus; Carcass traits; Chemerin gene; PCR-SSCP; SNP

Qinchuan is a red or yellow draft and beef breed in China. In order to identify a predictor of carcass traits on the basis of associations between carcass traits and gene polymorphism, variation in the bovine chemerin gene was investigated using PCR-single-strand conformational polymorphism and DNA sequencing. An SNP of A868G located in exon 2 of the Bos taurus chemerin gene was ... more

W.Q. Tian; H.C. Wang; F.B. Song; L.S. Zan; H. Wang; H.B. Wang; Y.P. Xin; J.A. Ujan
09/15/2011
Beef; Bovine; Genome; Livestock; Molecular markers; SNP

We analyzed single nucleotide polymorphisms in calpain, leptin, leptin receptor, and growth hormone receptor genes and their association with color, drip and cooking losses of longissimus muscle at 7, 14 and 21 days postmortem in 638 purebred Nellore bulls slaughtered between 22 and 26 months of age. Meat samples were vacuum-packed and aged at 4°C. The single nucleotide polymorphisms ... more

L.F.B. Pinto; J.B.S. Ferraz; V.B. Pedrosa; J.P. Eler; F.V. Meirelles; M.N. Bonin; F.M. Rezende; M.E. Carvalho; D.C. Cucco; R.C.G. Silva
09/09/2011
ACOX1 gene; Cattle; PCR-SSCP; SNP; Ultrasound traits

Acyl-coenzyme A oxidase 1 (ACOX1) is the first enzyme in peroxisomal fatty acid β-oxidation; it is rate-limiting and plays a key role in fatty acid metabolism and fat deposition. ACOX1 is an important candidate gene for meat quality selection through marker-assisted selection. Genomic structural analysis showed that bovine ACOX1 shares 86% identity with human ACOX1. Using PCR-SSCP ... more

Y. Jiao; L.S. Zan; Y.F. Liu; H.B. Wang
08/16/2011
IGF-1; PCR-SSCP; SNP

The IGF-1 gene has been implicated as a candidate gene for the regulation of pig growth traits. We analyzed exons 3 and 4 of IGF-1 gene polymorphisms of the Banna mini-pig (28), the Tibetan mini-pig (30), the Junmu pig (55), and L. Yorkshire species (50) using PCR-SSCP. Three genotypes in exon 3 and 6 genotypes in exon 4 were observed, among which, one single nucleotide polymorphism, ... more

L.L. Hao; H. Yu; Y. Zhang; S.C. Sun; S.C. Liu; Y.Z. Zeng; Y.X. Ai; H.Z. Jiang
05/31/2011
Body measurement traits; Cattle; GDF10 gene; SNP

Body measurement traits are known to play numerous important roles in the assessment of productivity and economic value. They are influenced by several factors, among which genetic factors are predominant. The gene GDF10 is involved in skeletal morphogenesis and is associated with body measurement traits. It may be an important candidate gene for marker-assisted selection. We ... more

C. Adoligbe; L.S. Zan; H.B. Wang; J.A. Ujjan

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