Research Article

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11/02/2008
Allozymes; Asteraceae; Campo rupestre; Cerrado; Eremanthus glomerulatus; mtDNA

Tomoplagia reticulata and T. pallens are sibling species that are specialists on Eremanthus glomerulatus. Besides adult terminalia, they show slight morphological differences and distinct geographic distributions. Once, however, they were found sympatrically. Using data from allozyme and mtDNA, we examined patterns of intra- and interspecific genetic structure, and ... more

A.G. Abreu; V.N. Solferini
03/01/2011
Cerrado; Dimorphandra mollis; DNA extraction; Metabolic compounds; “Fava d’anta”

Isolation of high-quality DNA from plants, especially plants from the Cerrado, is notoriously difficult because of polysaccharides and secondary compounds produced by plants from this biome. DNA isolation and its quality may be compromised by chemical defenses such as tannins and phenols. Quantitative plant defenses tend to have a cumulative effect, increasing in concentration during ... more

P.A. Moreira; D.A. Oliveira
09/08/2010
Cerrado; Loricariidae; Microsatellite; Shotgun library

We isolated and characterized 10 microsatellite loci in the armored catfish (Hypostomus gymnorhynchus, Loricariidae), using a genomic shotgun library to obtain the repetitive sequences. Twenty-four primers were designed and 14 individuals of H. gymnorhynchus from the Caiapó River, in central Brazil, were genotyped using these primers to analyze the polymorphism at each ... more

M.P.C. Telles; L.V. Resende; R.P.V. Brondani; R.G. Collevatti; M.C. Costa; N.J.Silva Júnior
08/03/2010
Cerrado; Erythrinidae; Microsatellite; Shotgun library

We identified 14 microsatellite loci for the wolf fish, Hoplias malabaricus (Erythrinidae), from a genomic shotgun library. Twenty-five primers were designed, and 48 individuals of H. malabaricus from four localities of northwest Goiás, in central Brazil, were genotyped to characterize the polymorphism at each locus. Fourteen primers amplified clearly interpretable ... more

S.G.C.A. Gondim; L.V. Resende; R.P.V. Brondani; R.G. Collevatti; N.J. Silva-Júnior; R.R. Pereira; M.P.C. Telles
11/27/2013
Alouatta caraya; Assignment test; Brazil; Cerrado; Pedigree; Philopatry

We here investigated the kin structure and pattern of dispersal in the black-and-gold howler monkey (Alouatta caraya, Platyrrhini, Atelidae) based on genotype differences at nine microsatellite loci of 48 individuals from eight social groups along the riparian forest of the Tocantins River, Brazil. The genetic diversity (HE = 0.647) was similar to or higher ... more

R.G. Collevatti; A.C. Souza-Neto; N.J. Silva-Jr.; M.P.C. Telles
09/12/2013
Cerrado; Conservation; Genetic variability; Germplasm; Multivariate techniques

The fragmentation of the original vegetation of the Cerrado biome, caused by the expansion of agricultural areas, mainly in central-west Brazil, calls for an assessment of the native population of this vegetation, especially of the species of interest for domestication and sustainable use. The purpose of this study was to characterize the genetic diversity of 140 gabiroba mother plants ( ... more

E.S. de Assis; E.Fdos Reis; J.F.N. Pinto; L.A.S. Contim; L.A.S. Dias
08/21/2013
Cagaita; Cerrado; Genetic diversity; Microsatellite; Shotgun library

Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localities. The number of alleles ... more

M.P.C. Telles; J.B. Silva; L.V. Resende; R.P. Vianello; L.J. Chaves; T.N. Soares; R.G. Collevatti
09/26/2012
Cerrado; Chiroptera; Conservation; Genetic diversity; Neotropical bat; Shotgun genomic library

Glossophaga soricina is a widespread Neotropical nectarivorous bat. We characterized 10 microsatellite loci isolated from a shotgun genomic library. We analyzed tissues from wing membrane of 67 individuals collected from two populations of Central Brazil (Brasília and Alto Paraíso). The number of alleles per locus ranged from 2 to 20, and the observed and expected ... more

M. Oprea; F.P. Peixoto; L.V. Resende; R.G. Collevatti; M.P.C. Telles
03/22/2012
Cerrado; Dimorphandra mollis; DNA extraction; High-quality DNA; Polysaccharide-free DNA; Secondary metabolites

Dimorphandra mollis (Leguminosae), known as faveiro and fava d’anta, is a tree that is widely distributed throughout the Brazilian Cerrado (a savanna-like biome). This species is economically valuable and has been extensively exploited because its fruits contain the flavonoid rutin, which is used to produce medications for human circulatory diseases. Knowledge ... more

H.A.V. Souza; L.A.C. Muller; R.L. Brandão; M.B. Lovato
09/30/2007
genetic relationships; Groundnut; Microsatellites; peanut; Simple sequence repeat

The Kayabi Indians who inhabit the Xingu Indigenous Park, located in West Central Brazil, have grown and managed peanuts for a long time. A great number of landraces are being maintained by these tribes and some of this germplasm has morphological traits that exceed the variation described in the taxonomic literature. Here, we analyzed the genetic variability of these landraces using a ... more

F.O. Freitas; M.C. Moretzsohn; J.F.M. Valls

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