Case Report

Related GMR Articles

Angiomatoid features; Cancer; Cytogenetics; Epithelioid sarcoma

Epithelioid sarcoma is a rare, aggressive soft tissue tumor of unknown histogenesis showing predominantly epithelioid cytomorphology. We conducted a conventional and molecular cytogenetic study of a 27-year-old male with epithelioid sarcoma with angiomatoid features. Cytogenetic analysis of epithelioid sarcoma metaphase spreads by GTG-banding revealed a diploid chromosome complement with ... more

M.S. Brassesco; E.T. Valera; A.M. Castro-Gamero; D.A. Moreno; T.P. Silveira; B.M. Mori; E.E. Engel; C.A. Scrideli; L.G. Tone
Cancer biology; Cytogenetics; Tumor biology; Wilms tumor

Genetic instability is frequent in human cancer. Unscheduled tetraploidization can trigger cell transformation and tumorigenesis. We made a cytogenetic analysis by Giemsa-trypsin banding of a stage I, biphasic Wilms tumor diagnosed in a 10-month-old male. An evident karyotypic heterogeneity was found. Four different subclones of tumor cells were observed, with DNA content varying from diploid ... more

E.T. Valera; M.S. Brassesco; C.A. Scrideli; L.G. Tone
Co-expression network; Hub gene; Pathway; Retinoblastoma; Reverse transcription-polymerase chain reaction

The objective of this paper was to identify hub genes and pathways associated with retinoblastoma using centrality analysis of the co-expression network and pathway-enrichment analysis. The co-expression network of retinoblastoma was constructed by weighted gene co-expression network analysis (WGCNA) based on differentially expressed (DE) genes, and clusters were obtained through the ... more

Q.L. Wang; X. Chen; M.H. Zhang; Q.H. Shen; Z.M. Qin
Bats; Cell culture; Chiroptera; Cytogenetics

We developed a procedure to obtain fibroblasts from bat skin. A small fragment of the ear is removed under ether anesthesia. This material is then cut up into small pieces and cultured in standard cell culture media. Very good quality chromosome preparations for cytogenetic studies are obtained in about three weeks. Secondary cultures can be used for other biological studies. This ... more

R. Moratelli; Cde Moraes Andrade; J.Luís Azev de Armada
Canova Method; Cytogenetics; Mitotic index; Mutagenesis

The Canova Methodâ (CM) is a homeopathic medicine indicated for the treatment of patients with cancer and for pathologies that involve a depressed immune system, such as AIDS. This product is composed of homeopathic dilutions of Aconitum napellus, Arsenicum album (arsenic trioxide), Bryonia alba, Lachesis muta venom and Thuya ... more

I.C. Seligmann; P.D.L. Lima; P.C.S. Cardoso; A.S. Khayat; M.O. Bahia; Dde Freitas Buchi; I.R. Cabral; R.R. Burbano
Chromosome evolution; Cratylia argentea; Cratylia mollis; Cytogenetics; Karyotype; tropical forage

Cratylia argentea and C. mollis (Fabaceae-Papilionoideae) are legume shrubs native to the Cerrado and Caatinga, respectively. Both species show great resistance to drought and high nutritive value, which makes them a valuable forage resource in tropical regions. Cytogenetic studies were carried out on accessions of C. argentea and C. mollis from ... more

S.M. Vargas; G.A. Torres; F.S. Sobrinho; A.V. Pereira; L.C. Davide
Cytogenetics; Diplopoda; Fluorochromes; Heterochromatin

Triple staining with fluorochromes (DA/DAPI/CMA) and C-banding were used to characterize the composition of Pseudonannolene strinatii heterochromatin. C-banding showed C+ bands of different labeling intensity on chromosomes 1 and 2 in some cells. Fluorochrome staining revealed DAPI+ regions corresponding to the C-banding pattern, indicating that the heterochromatin of this ... more

K.Agari Campos; C.Silvia Fontanetti
Classification; Cytogenetics; del(3)(p25); Myelodysplastic syndromes

The myelodysplastic syndromes (MDS) are clonal hematopoietic diseases characterized by medullary dysplasia, cytopenias, and frequent evolution to acute myeloid leukemia. In 1982, the French-American-British (FAB) group proposed a classification for the MDS, based on morphological characteristics of peripheral blood and of the bone marrow. Later, cytogenetics proved to be a useful ... more

G.Rebouças Pinto; D.James Overal; A.Virgínia Van Den Berg; J.Alexandre Lemos; Mde Arruda Smith; Mde Arruda Smith; R.Rodríguez Burbano; L.Silva de Moraes
Acute lymphoblastic leukemia; Chromosomal anomalies; Cytogenetics; Hybrid transcripts; Reverse transcriptase-polymerase chain reaction

Acute lymphoblastic leukemia (ALL) accounts for approximately 80% of all acute leukemias during childhood. Chromosomal anomalies resulting from gene fusion, which are frequent in leukemias, create hybrid transcripts, the great majority of which encode transcription factors. We analyzed 88 pediatric patients (median age 7.3 years) who had B-lineage acute lymphoblastic leukemia (B-ALL), using ... more

D.R. Mesquita; J.C. Córdoba; Í.Q. Magalhães; M.S. Córdoba; J.R.C. Oliveira; A. Gonçalves; I. Ferrari; C. Martins-de-Sá
B chromosome; Chromosomal polymorphism; Cytogenetics; Heterochromatin; Hypostominae

Hypostomus sp 3-Córrego Salobrinha NUP 4247 and Hypostomus sp 2-Rio Perdido NUP 4249, collected in the Planalto da Bodoquena, Paraguay River basin, Brazil, were characterized cytogenetically. Hypostomus sp 3-Córrego Salobrinha showed two modal numbers. This polymorphism consists of the presence of two extrachromosomes. It was not possible to define the diploid number ... more

S.S. Cereali; E. Pomini; R. Rosa; C.H. Zawadzki; O. Froehlich; L. Giuliano-Caetano