Research Article

Related GMR Articles

08/18/2009
Candidate gene; Genotyping; PCR-RFLP; POU1F

We examined the polymorphisms in the PIT1 gene of 103 pigs and compared their frequencies in the maternal and paternal lineages of the Pietrain and Large White breeds, which have undergone divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by PCR and genotyped by RFLP, using the restriction enzyme RsaI. The data were ... more

A.C.P. Silveira; T.F. Braga; J.F. Almeida; R.C. Antunes; P.F.A. Freitas; A.S.M. César; A.S.M. César; E.C. Guimarães
10/19/2011
Genotyping; Heterozygote deficiency; Microsatellite; Null alleles; Parentage; White-tailed deer

Microsatellites are useful tools for ecological studies because they can be used to discern population structure, dispersal patterns and genetic relationships among individuals. However, they can also yield inaccurate genotypes that, in turn, bias results, promote biological misinterpretations, and create repercussions for population management and conservation programs. We used empirical ... more

A.C. Kelly; N.E. Mateus-Pinilla; M. Douglas; M. Douglas; P. Shelton; J. Novakofski
11/03/2010
Diversity; Genotyping; Prunus; Rootstock; SSR

Ten SSR loci, previously developed for Prunus, were analyzed to examine genetic relationships among 23 rootstock candidates for sweet and sour cherries, of the species P. avium, P. cerasus, P. mahaleb, and P. angustifolia. Five genotypes of P. laurocerasus, not used as rootstock, were included in the molecular analysis. The number of ... more

Z. Turkoglu; S. Bilgener; S. Ercisli; M. Bakır; M. Bakır; A. Koc; M. Akbulut; R. Gercekcioglu; M. Gunes; A. Esitken
11/07/2013
Chrysanthemum; Genotyping; Marker development; Population structure; SSR

Chrysanthemums are well known for their esthetic and medicinal values. Characterization of chrysanthemums is vital for their conservation and management as well as for understanding their genetic relationships. We found 12 simple sequence repeat markers (SSRs) of 100 designed primers to be polymorphic. These novel SSR markers were used to evaluate 95 accessions of chrysanthemums (3 ... more

A.A. Khaing; K.T. Moe; W.J. Hong; C.S. Park; K.H. Yeon; H.S. Park; D.C. Kim; B.J. Choi; J.Y. Jung; S.C. Chae; K.M. Lee; Y.J. Park
08/12/2013
Genotyping; Haplotype analysis; IL-10 gene promoter; Single nucleotide polymorphism

The purpose of this study was to determine whether interleukin-10 (IL-10) promoter polymorphisms are associated with leprosy or their subtypes in ethnic groups from southwest China. Genotyping using TaqMan® SNP Genotyping Master Mix and ABI 7500 real-time PCR system was performed for IL-10 T3575A, G2849A, C2763A, A1082G, C819T, and C592A in 189 healthy controls (40 ± 18 years) and 193 ... more

X.H. Chen; J.H. Xiong; Y. Ning; Y. Wen; J. Liu; C. Mao; X.M. Weng; J.C. Gu
04/25/2013
F. culmorum; F. graminearum; Fusarium head blight; Genotyping; Random-amplified polymorphic DNA

Five Fusarium graminearum and 12 F. culmorum isolates, primarily pathogenic species of Fusarium head blight, were obtained from naturally infected wheat from various agro-ecological regions of Turkey. Genotyping of the isolates was carried out using random-amplified polymorphic DNA (RAPD). Sixty-five 10-mer oligonucleotide primers were used to amplify the RAPD ... more

E. Yörük; G. Albayrak
05/21/2012
Genotyping; Germplasm; Molecular markers; Simple sequence repeats; Tvv1; Vitis spp

Identification and knowledge concerning genetic diversity are fundamental for efficient management and use of grapevine germplasm. Recently, new types of molecular markers have been developed, such as retrotransposon-based markers. Because of their multilocus pattern, retrotransposon-based markers might be able to differentiate grapevine accessions with just one pair of primers. In order ... more

G.C. Sant’Ana; J.L. Ferreira; H.S. Rocha; A. Borém; M. Pasqual; G.M.A. Cançado
04/08/2014
Coronary artery disease; Genotyping; Polymorphism; Predisposing genes; Single nucleotide

This study investigated 5 single nucleotide polymorphism (SNP) haplotypes in susceptibility genes for coronary artery disease (CAD) and the putative involvement of these SNPs in CAD in the Chi­nese Han population. From March 2008 to June 2009, we selected 119 CAD patients and 115 subjects not related to the CAD of Chinese Han or­igin as controls. The SNP genotypes were performed by ... more

Y.H. Liu; Y.W. Zhou; J.A. Yang; Z.G. Tu; S.Y. Ji; Z.Y. Huang; Z.J. Zhou
02/27/2014
Acroporidae; Gene flow; Genetic markers; Genotyping; Marker transferability; South-east Africa

Here, we report the successful cross-species amplification of previously published acroporid microsatellite markers in the coral Acropora austera from the south-western Indian Ocean. This fast-growing species is a major reef-building coral on South African reefs; however, it is the most damaged coral by scuba diving activity, and is known to be very susceptible to coral bleaching ... more

P.H. Montoya-Maya; A.H.H. Macdonald; M.H. Schleyer
05/11/2015
Baller-Gerold syndrome; Genotyping; Prenatal diagnosis; RECQL4

Birth defects are structural and/or functional malforma­tions present at birth that cause physical or mental disability and are im­portant public health problems. Our study was aimed at genetic analysis and prenatal diagnosis of congenital anomalies to understand the cause of certain birth defects. Karyotypes and array-comparative genomic hy­bridization (aCGH) were performed on a pregnant ... more

D.H. Cao; K. Mu; D.N. Liu; J.L. Sun; X.Z. Bai; N. Zhang; G.B. Qiu; X.W. Ma

Pages