Research Article

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10/05/2007
FISH; Genomic library; Jackknife estimate; Microsatellites; Piracema species; Prochilodus lineatus

We described five novel microsatellite loci for the piracema fish species Prochilodus lineatus (Characiformes), endemic to South America and of extreme importance to both commercial and artisanal fisheries across its occurrence area. A primary, unenriched genomic library was constructed and radioactively screened for repetitive motifs. Positive clones were automatically ... more

G.M. Yazbeck; E. Kalapothakis
05/22/2007
Blastocerus dichotomus; Cervidae; Marsh deer; Microsatellites; Transferability

Blastocerus dichotomus, the marsh deer, is the largest Brazilian Cervidae species. The species is endangered because of hunting and loss of its natural habitat, i.e., flood plain areas, because of hydroelectric power station construction and agricultural land expansion. In the present study, we tested 38 microsatellite loci from four Cervidae species: Odocoileus ... more

K.C.E. Leite; R.G. Collevatti; T.R. Menegasso; W.M. Tomas; J.M.B. Duarte
05/31/2007
Genetic variability; Microsatellites; Nuclear molecular markers; Population structure; Wading bird

Roseate Spoonbills (Platalea ajaja, Linnaeus) are wading birds present in two of the most important Brazilian wetlands: the Pantanal wetlands and Rio Grande do Sul marshes. Natural populations of these species have not been previously studied with variable nuclear molecular markers. In order to support decision making regarding the management and conservation of these populations, we ... more

C.I. Miño; S.N. Del Lama
12/05/2006
CG content; Hyperthermophiles; Microsatellites; Optimum growth temperature; SSRs; Thermophiles

All organisms that have been studied until now have been found to have differential distribution of simple sequence repeats (SSRs), with more SSRs in intergenic than in coding sequences. SSR distribution was investigated in Archaea genomes where complete chromosome sequences of 19 Archaea were analyzed with the program SPUTNIK to find di- to penta-nucleotide repeats. The number of ... more

S. Trivedi
03/22/2007
Brazil; Microsatellites; Mollusca; Mytilidae; Perna perna; Population genetics

Perna perna is the most important cultivated mussel of Santa Catarina, Brazil, sustaining an important economic input for many local families. Natural stocks of P. perna are depleted by the extraction of adults and seeds for consumption and culture. The aim of the present study was to use the microsatellite locus pms-2 to study the variation of the genetic ... more

K.T. Appio; L.I. Weber
07/13/2006
Ancestry; Microsatellites; Synthetic breed; Taurine; Zebu

The International Society of Animal Genetics (ISAG) has chosen nine microsatellites (international marker set) as a standard that should be included in all cattle parentage studies. They are BM1824, BM2113, INRA023, SPS115, TGLA122, TGLA126, TGLA227, ETH10, and ETH225. We decided to ascertain whether this microsatellite set could be used to determine ancestral proportions in ... more

H.M.S. Bicalho; C.G. Pimenta; I.K.P. Mendes; H.B. Pena; E.M. Queiroz; S.D.J. Pena
06/22/2006
matUPD15; Microsatellite analysis; Microsatellites; Prader-Willi syndrome

Prader-Willi syndrome (PWS) is a multisystemic disorder caused by the loss of expression of paternally transcribed genes in the PWS critical region of chromosome 15. Various molecular mechanisms are known to lead to PWS: deletion 15q11-q13 (75% of cases), maternal uniparental disomy (matUPD15) (23%) and imprinting defects (2%). FISH and microsatellite analysis are required to ... more

H.V. Aráoz; M. Torrado; C. Barreiro; L. Chertkoff
12/30/2005
Cebus apella; DNA polymorphisms; Microsatellites; Population structure; Short tandem repeats; Tufted capuchins

There have been numerous studies genetically characterizing Old World Primates using microsatellites. However, few studies have been made of New World species and none on free-ranging Cebus apella, even though it is probably the most widely distributed species of monkey in the New World. The paucity of studies is due, in part, to the lack of polymorphisms described for this species. We ... more

J.Margareth Amaral; A.Luiz Simões; D. De Jong
10/27/2009
Disease index; Leaf disc test; Microsatellites; Phytophthora palmivora; Quantitative trait loci; Theobroma cacao
E.S.L. Santos
03/18/2008
Genetic variability; Horse; Microsatellites; Pantaneiro horses

The genetic variability for a sample of 227 animals from three populations of Pantaneiro horses was estimated using data from 10 microsatellite loci. The number of alleles and the proportion of heterozygosity indicated high variability. A total of 91 alleles were found, with a significantly high mean number of alleles. The mean polymorphic information content was 0.7 and the paternity ... more

E.H. Giacomoni; G.P. Fernández-Stolz; T.R.O. Freitas

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