Research Note

Related GMR Articles

EST-SSR; Genetic diversity; SSR; Tomato varieties

The genetic diversity and relationship of 42 tomato varieties sourced from different geographic regions was examined with EST-SSR markers. The genetic diversity was between 0.18 and 0.77, with a mean of 0.49; the polymorphic information content ranged from 0.17 to 0.74, with a mean of 0.45. This indicates a fairly high degree of diversity among these tomato varieties. Based on the ... more

N.K. Korir; W. Diao; R. Tao; X. Li; E. Kayesh; A. Li; W. Zhen; S. Wang
Cultivar identification; Genetic diversity; Genetic similarity; ISSR; Olea europaea L.; SSR

The olive tree is an iconic tree of the Mediterranean, and is used extensively to produce high-quality olive oil. Although the China olive industry has just begun to be valued, there were also existed mislabeling and synonyms in introduced cultivars. The aim of this study was to analyze genetic similarities among olive cultivars in China using SSR and ISSR techniques. Thirty-two samples ... more

M.M. Zhan; Z.Z. Cheng; G.C. Su; A.Y. Wang; H.P. Chen; Z.S. Yang; Z. Shan; Q.M. Huang
Cross-amplification; Lycoris radiata; Microsatellite; Molecular marker

Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs using a modified CTAB protocol. We isolated 10 ... more

S.Q. Xuan; J.Y. Zheng; H. Wang; P. Gao; S.B. Zhou; K. Liu; G.P. Zhu
Cross-amplification; Microsatellite markers; Nyctereutes procyonoides procyonoides

Chinese raccoon dog (Nyctereutes procyonoides procyonoides) is one of the most important fur-bearing animal species. Information about the genetic background of farmed Chinese raccoon dogs is limited. In this study, 17 polymorphic microsatellite markers were isolated and identified from an (AC)n-microsatellite-enriched library of Chinese raccoon dogs. The number of ... more

S.Q. Yan; Y.M. Li; C.Y. Bai; X.M. Ding; W.J. Li; J.N. Hou; Z.H. Zhao; J.H. Sun
Anopheles albitarsis; Cross-amplification; Malaria vectors; Microsatellite-enriched library; Species complex; SSR

The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897 ... more

G.M. Guimarães-Marques; J.S. Batista; H.M. Guimarães; M.V. Naice-Daou; M.P. Lima; K.M. Formiga; J.M.M. Santos; C.A.P. Lima; M.S. Rafael
Cross-amplification; Fluorescence in situ hybridization; Forage; Microsatellite

Aeschynomene falcata is an important forage species; however, because of low seed production, it is underutilized as forage species. Aeschynomene is a polyphyletic genus with a challenging taxonomic position. Two subgenera have been proposed, and it is suggested that Aeschynomene can be split in 2 genera. Thus, new markers, such as microsatellite sequences, are ... more

C.A. Polido; C.C. Mantello; A.P. Moraes; A.P. Souza; E.R. Forni-Martins
Cross-amplification; Microsatellite markers; Population genetics; Siniperca chuatsi

The mandarin fish is a popular fresh water food fish in China. Fifty-three polymorphic microsatellite markers were isolated through construction of an enriched library of genomic DNA of Siniperca chuatsi (Percichthyidae). We found 2 to 7 alleles per locus. The observed and expected heterozygosity values varied from 0.059 to 1.000 and from 0.305 to 0.818, respectively. The ... more

C.X. Tian; X.F. Liang; M. Yang; Y.Q. Dou; H.Z. Zheng; L. Cao; Y.C. Yuan; C. Zhao
Cross-amplification; Microsatellite markers; Paramisgurnus dabryanus; Polymorphism

The large-scale loach, Paramisgurnus dabryanus, is a small freshwater fish of major economic importance in many Asian countries, particularly China and South Korea. Fifteen polymorphic microsatellite (simple sequence repeat) markers were obtained through cross-species amplification between this loach and a related species, Misgurnus anguillicaudatus (GenBank accession numbers: KC117456 to ... more

G. Chen; S. Huang; J. Gao; X. Bai; W. Wang; X. Cao
Genetic diversity; RAPD; Triticum aestivum L.

The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. Among the 112, 50 fragments showed polymorphism among the seven wheat genotypes. Nei and Li’s ... more

A. Iqbal; A.S. Khan; I.A. Khan; F.S. Awan; A. Ahmad; A.A. Khan
Evolution; Exotic animals; Genetic diversity; Molecular genetic

We examined the variation in mitochondrial DNA by sequencing the D-loop region in wild and domestic (large-white breed) pigs, in hybrids between domestic and wild pigs, and in Monteiro pigs. A D-loop fragment of approximately 330 bp was amplified by PCR. Sequencing of DNA amplicons identified haplotypes previously described as European and Asian types. Monteiro pigs and wild pigs had ... more

S.F. Grossi; J.F. Lui; J.E. Garcia; F.V. Meirelles