Research Note

Related GMR Articles

Cross-amplification; Genetic variability; SSR; Transferability

Leporinus friderici, native to the Amazon Basin and popularly known as “piau-três-pintas”, has great ecological and economic importance; it is widely fished and consumed throughout much of tropical South America. Knowledge of the genetic diversity of this native species is important to support management and conservation programs. We evaluated microsatellite loci amplification, ... more

A.M. Olivatti; T.A. Boni; N.J. Silva-Júnior; L.V. Resende; F.O. Gouveia; M.P.C. Telles
China; EST-SSR; Genetic diversity; Saccharum spontaneum L.; SSR

Saccharum spontaneum is the most variable wild relative of sugarcane with potential for use in sugarcane improvement programs. In order to help preserve and exploit this species, 152 accessions from eight major geographical regions in China, including Hainan, Guangdong, Guangxi, Yunnan, Sichuan, Guizhou, Fujian, and Jiangxi provinces, were investigated by analyzing 20 simple ... more

L.N. Fan; H.H. Deng; Q.W. Luo; H.Y. He; Y. Li; Q.N. Wang; Z.X. Huang; J.T. Wu; Q.W. Li; S.M. Liu; Y.W. Qi
Cydonia oblonga; Genetic diversity; Quince; SSR

We conducted simple sequence repeat (SSR) analyses of 15 traditional quince (Cydonia oblonga) cultivars from Anatolian gene sources for molecular characterization and investigation of genetic relationships. Three pear and two apple cultivars were used as references for SSR locus data analysis and to determine allele profiles between species. Eight SSR loci that were developed ... more

C. Yüksel; F. Mutaf; İ. Demirtaş; G. Öztürk; M. Pektaş; A. Ergül
Cherry; East Anatolia; Genetic diversity; Plum; SSR

We conducted SSR analyses of 59 accessions, including 29 traditional plum (Prunus domestica), 24 sweet cherry (Prunus avium), and 1 sour cherry (Prunus cerasus) selected from East Anatolian gene sources and 3 plum and 2 cherry reference accessions for molecular characterization and investigation of genetic relationships. Eight SSR loci [1 developed from the ... more

M.H. Öz; H. Vurgun; M. Bakir; İ. Büyük; C. Yüksel; H.M. Ünlü; K. Çukadar; B. Karadoğan; Ö. Köse; A. Ergül
Genetic diversity; Lamiaceae; Pogostemon cablin; SSR

Microsatellite primers were developed and optimized for patchouli (Pogostemon cablin) to characterize the patchouli Active Germplasm Bank of Universidade Federal de Sergipe. Creation of a genomic library for patchouli enabled the design of 12 microsatellite primers. Six of these microsatellites were polymorphic, revealing two well-defined groups of individuals that ... more

S.S. Sandes; J.B. Pinheiro; M.I. Zucchi; M. Monteiro; M.F. Arrigoni-Blank; A.F. Blank
Chinese wild grape; Genetic diversity; SRAP; SSR; Vitis

The genetic diversity among 80 Vitis materials including 62 indigenous accessions of 17 wild Vitis species in China and 7 interspecific hybrids, 10 V. vinifera L. cultivars, and 1 V. riparia Michaux were evaluated by simple sequence repeat and sequence-related amplified polymorphism markers. A total of 10 simple sequence repeat primers and 11 sequence- ... more

Z.B. Jing; X.P. Wang; J.M. Cheng
cluster analysis; Cynodon dactylon; Genetic diversity; SSR

Fifty-five wild accessions of bermudagrass (Cynodon dactylon) were collected from southwest China (Sichuan, Chongqing, Yunnan, Guizhou, and Tibet), and their genetic diversity was analyzed using simple sequence repeat markers. A total of 267 polymorphic bands were detected with 18 primer combinations. The genetic similarity among the accessions ranged from 0.688 to 0.894 with an ... more

Y. Ling; X.Q. Zhang; X. Ma; S.Y. Chen; T.T. Chen; W. Liu
cluster analysis; Genetic diversity; Lagerstroemia; SSR

Lagerstroemia (crape myrtle) are famous ornamental plants with large pyramidal racemes, long flower duration, and diverse colors. However, little is known about the genetic structure and diversity of germplasm in Lagerstroemia. We genotyped 81 L. indica cultivars, five other species of Lagerstroemia, and 10 interspecific hybrids using 30 simple sequence ... more

D. He; Y. Liu; M. Cai; H.T. Pan; Q.X. Zhang; X.Y. Wang; X.J. Wang
Genetic diversity; Genetic structure; SSR

Nine microsatellite loci were isolated from the genome of Laodelphax striatellus (Homoptera: Delphacidae) by constructing (TC)6(AC)5 and (AG)6(AC)5 compound SSR-enriched libraries using suppression-PCR procedures. These loci were found to be highly polymorphic, with 13 to 30 alleles per locus in the three populations that we investigated ... more

J.T. Sun; J.B. Li; X.M. Yang; X.Y. Hong
DNA fingerprinting; Fruit-endocarp or fruit-pit characters; Genetic diversity; Olea europaea; SSR

Seventy-seven olive accessions corresponding to 25 cultivars from the Extremadura region of Spain were studied using four microsatellite or SSR markers in order to fingerprint them, and evaluate genetic similarity and relationships between local and introduced olive cultivars. The number of alleles per locus ranged from 4 to 8, with a mean of 6.25 alleles per primer pair (a total of 25 ... more

F.J. Delgado-Martinez; I. Amaya; J.F. Sánchez-Sevilla; M.C. Gomez-Jimenez