Research Article

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08/30/2011
Canopy form; Cypress; Fastigate; Pseudo-test cross; RAPD; SCAR

Gene mapping for a Cupressus species is presented for the first time. Two linkage maps for the Mediterranean cypress (Cupressus sempervirens) varieties, C. sempervirens var. horizontalis and C. sempervirens var. pyramidalis, were constructed following the pseudo-testcross mapping strategy and employing RAPD, SCAR and morphological ... more

C. Manescu; Ν. Hamamouch; C. Maios; A. Harfouche; A.G. Doulis; F.A. Aravanopoulos
10/18/2013
marker-assisted selection; Psathyrostachys huashanica; RAPD; Repetitive sequence; SCAR; Wheat

In this study, we cloned and sequenced a 938-base pair polymorphic band, pHs27, in the tightly linked random amplified polymorphic DNA marker OPU10 and converted it into a sequence-characterized amplified region (SCAR) marker referred to as RHS141, which was specific for the Ns genome of Psathyrostachys huashanica. A GenBank basic local alignment search tool search showed that ... more

W.L. Du; J. Wang; L.M. Wang; Y.H. Pang; J. Wu; J.X. Zhao; Q.H. Yang; X.H. Chen
02/24/2012
Cultivar discrimination; DNA marker; RAPD; Rosa; SCAR

DNA fingerprints of four rose species, Rosa centifolia, R. Gruss-an-Teplitz, R. bourboniana, and R. damascena, were developed using RAPD-PCR. We identified a unique polymorphic band in R. centifolia. This 762-bp fragment was produced by the random primer GLI-2. The fragment was eluted and directly cloned in a TA cloning vector, pTZ57R/T. ... more

S. Riaz; B. Sadia; F.S. Awan; I.A. Khan; H.A. Sadaqat; I.A. Khan
05/30/2014
Cloning; RAPD; Rose; SCAR; Sequencing

Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the influence of environmental factors, which is ... more

K.M.I. Bashir; F.S. Awan; I.A. Khan; A.I. Khan; M. Usman
06/14/2011
Chlorophyll accumulation; RAPD; Relative electrolyte leakage; SDS-PAGE

Cotton (Gossypium hirsutum) is an important cash crop, but high temperature during its growing season is one of the major factors that limit its productivity. This problem compels plant breeders to breed for heat tolerance, which can help to overcome this challenge. It is very important to make a comprehensive screening of heat-tolerant genotypes so that only the best are chosen ... more

R.M. Rana; S.H. Khan; Z. Ali; A.I. Khan; I.A. Khan
05/17/2011
Genetic diversity; Mycelial compatibility groups; RAPD; Sclerotinia sclerotiorum; White mold

The genetic variability of 40 Sclerotinia sclerotiorum isolates from various fields widely distributed throughout Brazil and different host crops was analyzed using RAPD markers and mycelial compatibility groupings (MCGs). The isolates were characterized using 16 random primers of the OPERON series, which produced 121 DNA fragments. UPGMA cluster analysis using Jaccard’s genetic ... more

C.G.Litholdo Júnior; E.V. Gomes; L. Júnior; L.C.B. Nasser; S. Petrofeza
02/23/2010
Genotype; RAPD; Resistance; Varroa destructor

The mite Varroa destructor is the main pest causing damage to apiculture worldwide. In Brazil and other parts of the world, where bees of African origin and their hybrids predominate, the bees can survive these mites without treatment. Studies have shown a correlation between the various genotypes of the mite and its fertility in different geographical regions. Information about ... more

J.C.V. Guerra; M.R.C. Issa; F.E. Carneiro; R. Strapazzon; G. Moretto
12/30/2002
Bipolaris sorokiniana; Genetic variability; RAPD; Wheat

Isolates of Bipolaris sorokiniana were analyzed by random-amplified polymorphic DNA (RAPD) techniques to determine the amount of intraspecific genetic variability and to study host-pathogen interactions. Ten isolates originated from different regions of Brazil were examined. Plants of the wheat cultivars BR8, BH1146 (original host) and IAC-5 Maringá, classified as resistant ... more

A.T.S. Matsumura; A.M. Prestes; S.T. Van Der Sand; A.M.R. de Oliveira
10/05/2007
CTAB; DNA extraction protocol; PVP; RAPD; Sunflower

In order to investigate the mutation characteristics and to further examine the genetic variation of mutant sunflower (Helianthus annuus) obtained in plants grown from seeds exposed to space conditions, only the mature tissues such as leaf and flower could be used for DNA extraction after mutation characteristics were confirmed. The rich contents of polysaccharides, tannins ... more

J.T. Li; D.C. Chen; X.L. Zhang; Z.S. Tang; J. Yang
08/07/2007
Genetic diversity; RAPD; Triticum aestivum L.

The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. Among the 112, 50 fragments showed polymorphism among the seven wheat genotypes. Nei and Li’s ... more

A. Iqbal; A.S. Khan; I.A. Khan; F.S. Awan; A. Ahmad; A.A. Khan

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