Short Communication

Related GMR Articles

12/30/2004
Alternative splicing; SAGE; Tumorigenesis

Although alternative splicing of many genes has been found associated with different stages of tumorigenesis and splicing variants have been characterized as tumor markers, it is still not known whether these examples are sporadic or whether there is a broader association between the two phenomena. In this report we evaluated, through a bioinformatics approach, the expression of ... more

N. Kirschbaum-Slager; G.M.P. Lopes; P.A.F. Galante; G.J. Riggins; S.J. de Souza
12/21/2011
Alternative splicing; Dairy cattle; Lactoferrin gene; Mastitis

Bovine lactoferrin (bLF) is a member of the transferrin family; it plays an important role in the innate immune response. We identified novel splice variants of the bLF gene in mastitis-infected and healthy cows. Reverse transcription-polymerase chain reaction (RT-PCR) and clone sequencing analysis were used to screen the splice variants of the bLF gene in the mammary ... more

J.M. Huang; Z.Y. Wang; Z.H. Ju; C.F. Wang; Q.L. Li; T. Sun; Q.L. Hou; S.Q. Hang; M.H. Hou; J.F. Zhong
09/27/2013
Alternative splicing; Bull; Cow; Liver; PEPCK gene; Testis

Phosphoenolpyruvate carboxykinase 1 (PCK1), also named PEPCK-C, is a multiple-function gene that is involved in gluconeogenesis, glyceroneogenesis, reproduction, female fertility, and development of obesity and diabetes. How its many functions are regulated was largely unknown. Therefore, we investigated mRNA expression and possible splice variants of PCK1 ... more

Z.B. Zhang; W. Zhang; R.L. Li; J.B. Li; J.F. Zhong; Z.S. Zhao; J.M. Huang
08/29/2012
Alternative splicing; C4A gene; Dairy cattle; Mastitis; Transcription pattern

The complement system helps in the direct lysis of invading pathogens and modulates phagocytic, humoral and cellular immune responses. Complement 4 is a critical component in complement activity and protection against many bacterial pathogens because it is essential to classical and lectin activation pathways. We used reverse transcription and PCR to investigate alternative splicing and ... more

Y. Yang; J.M. Huang; Z.H. Ju; Q.L. Li; L. Zhou; R.L. Li; J.B. Li; F.X. Shi; J.F. Zhong; C.F. Wang
03/17/2014
Alternative splicing; deletion; Dephosphorylation; GluR-B minigene; NSSR1

Neural salient serine/arginine-rich protein 1 (NSSR1, alternatively SRp38) is an important splicing factor that can repress pre-mRNA alternative splicing in cells during heat shock and mitosis. We show here that NSSR1 protein is dephosphorylated when cells are heat shocked or incubated with kinase inhibitor K252a. Both heat shock and K252a treatment increase the truncated splicing isoform ... more

W. Zhang; L.L. Li; X.H. Chen; Z.Y. Peng
01/23/2015
Alternative splicing; CAPN3; Cattle; Transcript

Calpain-3 (CAPN3) is a member of the calpain family of Ca2+-regulated cysteine proteases, which play an important role in sarcomere remodeling and mitochondrial protein turnover, and thus, regulating beef tenderness in cattle. Currently, multiple CAPN3 transcripts have been detected in human, monkey, rat, and rabbit. However, whether this transcript is present in cattle remains ... more

S.Y. Liu; H. Jiang; B. Yuan; Y. Gao; L.S. Dai; J.B. Zhang
12/30/2004
Alternative splicing; Bioinformatics; Exon skipping; Expressed sequences; Extracellular matrix; Human transcriptome

Alternative splicing increases protein diversity through the generation of different mRNA molecules from the same gene. Although alternative splicing seems to be a widespread phenomenon in the human transcriptome, it is possible that different subgroups of genes present different patterns, related to their biological roles. Analysis of a subgroup may enhance common features of its ... more

N.Jo Sakabe; M.Dulcetti Vibranovski; S.José de Souza
09/30/2008
Alternative splicing; Heteroduplex; methodology; S1 nuclease

The identification of alternatively spliced transcripts has contributed to a better comprehension of developmental mechanisms, tissue-specific physiological processes and human diseases. Polymerase chain reaction amplification of alternatively spliced variants commonly leads to the formation of heteroduplexes as a result of base pairing involving exons common between the two variants. S1 ... more

E.N. Ferreira; M.C.R. Rangel; P.B. Pineda; D.O. Vidal; A.A. Camargo; S.J. Souza; D.M. Carraro
04/08/2008
Antigenic characterization; Cloning; Expression; GRA5; GRA7; ROP2; Toxoplasma gondii

Toxoplasma gondii is an intracellular obligate protozoan,which infects humans and warm-blooded animals. The aim of the present study was to clone the rop2, gra5 and gra7 genes from T. gondii RH strain and to produce recombinant proteins. The rop2, gra5 and gra7 gene fragments produced by polymerase chain reaction were ... more

M. Igarash; F. Kano; K. Tamekuni; P.M. Kawasaki; I.T. Navarro; O. Vidotto; M.C. Vidotto; R.Z. Machado; J.L. Garcia
10/31/2011
Cloning; Gene expression; Granulocyte colony-stimulating factor; Recombinant G-CSF; Synthetic cDNA

Recently, granulocyte colony-stimulating factor (G-CSF) has been recognized as a useful molecule for the treatment of a wide range of complex ailments, such as cancer, AIDS, H1N1 influenza, cardiac and neurological diseases. The vast therapeutic potential of G-CSF has induced scientists to develop biotechnological approaches for the synthesis of this pharmacologically active agent. We ... more

S. Alrokayan

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