Research Article

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03/31/2004
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Chromobacterium violaceum is a versatile, Gram-negative β-protebacterium that grows in a variety of ecosystems in tropical and subtropical areas, such as the water and borders of the Negro River, in the Amazon region of Brazil. Although it is a saprophyte and is generally considered non-pathogenic, sporadic cases of human infection have been described, mainly in young children and in ... more

C.F.Alves de Brito; C.M.B. Carvalho; F.R. Santos; R.T. Gazzinelli; S.C. Oliveira; V. Azevedo; S.M.R. Teixeira
02/28/2004
Brazil; Genome; Network; Sequencing

Scientific research plays a fundamental role in the health and development of any society, since all technological advances depend ultimately on scientific discovery and the generation of wealth is intricately dependent on technological advance. Due to their importance, science and technology generally occupy important places in the hierarchical structure of developed societies, and ... more

A.J.G. Simpson; A.A. Camargo; J.Aparecido Ferro; J. Parra; A.Tereza Vasconcelos
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Genetic distance; Genome; Microsatellite; Molecular markers; Population genetics; Triticum aestivum

We investigated the genetic diversity of 63 wheat genotypes, composed of 48 accessions and 15 varieties, using 56 polymorphic simple sequence repeat primers. One hundred and eighty-six loci were found, with a mean of 131.26 alleles per locus. Cluster analysis based on microsatellite allelic diversity discrimi­nated the accessions and varieties into different clusters; genetic di­versity ... more

S. Ijaz; I.A. Khan
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Beef; Bovine; Genome; Livestock; Molecular markers; SNP

We analyzed single nucleotide polymorphisms in calpain, leptin, leptin receptor, and growth hormone receptor genes and their association with color, drip and cooking losses of longissimus muscle at 7, 14 and 21 days postmortem in 638 purebred Nellore bulls slaughtered between 22 and 26 months of age. Meat samples were vacuum-packed and aged at 4°C. The single nucleotide polymorphisms ... more

L.F.B. Pinto; J.B.S. Ferraz; V.B. Pedrosa; J.P. Eler; F.V. Meirelles; M.N. Bonin; F.M. Rezende; M.E. Carvalho; D.C. Cucco; R.C.G. Silva
07/30/2013
Chloroplast; Fragaria vesca; Genome; Molecular marker; Simple sequence repeats

The use of simple sequence repeats (SSRs), or microsatellites, as genetic markers has become popular due to their abundance and variation in length among individuals. In this study, we investigated linkage groups (LGs) in the woodland strawberry (Fragaria vesca) and demonstrated variation in the abundances, densities, and relative densities of mononucleotide, dinucleotide, and ... more

L. Guan; J.F. Huang; G.Q. Feng; X.W. Wang; Y. Wang; B.Y. Chen; Y.S. Qiao
09/02/2008
Chromosome aberrations; Dengue; Dillapiol; Micronucleus test

Dillapiol, a phenylpropanoid isolate from essential oils of leaves of Piper aduncum (Piperaceae), has insecticidal, fungicidal and antimicrobial activities. The insecticidal activity of dil lapiol was tested in vivo on the larvae and pupae of Aedes aegypti, the mosquito vector of dengue. Specifically, the effect of dillapiol on the formation of micronuclei and ... more

M.S. Rafael; W.J. Hereira-Rojas; J.J. Roper; S.M. Nunomura; W.P. Tadei
03/31/2004
Chromobacterium violaceum; DNA repair; Free-living organism; Genome

Chromobacterium violaceum is a Gram-negative β-proteobacterium that inhabits a variety of ecosystems in tropical and subtropical regions, including the water and banks of the Negro River in the Brazilian Amazon. This bacterium has been the subject of extensive study over the last three decades, due to its biotechnological properties, including the characteristic violacein ... more

F.Teixeira Duarte; F.Marques de Carvalho; U.Bezerra e Silva; K.Castanho Scortecci; C.Alfredo Ga Blaha; L.Fassarella Agnez-Lima; S.Regina Bat de Medeiros
03/31/2004
Bacterium; Biotechnology; Genome; Membrane; Transporters

The complete genome sequence of the free-living bacterium Chromobacterium violaceum has been determined by a consortium of laboratories in Brazil. Almost 500 open reading frames (ORFs) coding for transport-related membrane proteins were identified in C. violaceum, which represents 11% of all genes found. The main class of transporter proteins is the primary active ... more

T.Barbosa Grangeiro; D.Macedo de Jorge; W.Melgaço Bezerra; A.Tereza Rib Vasconcelos; A.John Georg Simpson
02/01/2008
Cow; Domestication; Exaptation; FASTKD3; Genome; Transposon

Despite the wide distribution of transposable elements (TEs) in mammalian genomes, part of their evolutionary significance remains to be discovered. Today there is a substantial amount of evidence showing that TEs are involved in the generation of new exons in different species. In the present study, we searched 22,805 genes and reported the occurrence of TE-cassettes in coding sequences of ... more

L.M. Almeida; M.E.J. Amaral; I.T. Silva; W.A. Silva; P.K. Riggs; C.M. Carareto
03/24/2014
Genome; Simple sequence repeat; Siniperca kneri Garman; Transcriptome

In this study, 37 transcriptome-derived simple sequence repeat (SSR) markers and 18 genomic SSR markers were developed and characterized in the Chinese perch, Siniperca kneri Garman. The average allele number per locus was 5.1 (range: 2-8) for transcriptome-derived SSRs and 3.8 (range: 2-5) for genomic SSRs. The average observed and expected heterozygosities were 0.666 (range: 0. ... more

M. Yang; H.Z. Zheng; X.F. Liang; C.X. Tian; Y.Q. Dou; K.C. Zhu; Y.C. Yuan

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