Research Article

Related GMR Articles

04/02/2013
Genetic association; Genetic polymorphism; Holstein cows; Molecular markers

The bovine prolactin (PRL) and bovine growth hormone (bGH) genes exhibit several polymorphisms. Some of them can be detected by molecular techniques using restriction endonucleases, such as RsaI for the PRL gene and MspI for the bGH gene. We examined the relationship between the PRL-RsaI and bGH-MspI polymorphisms and ... more

J.C. Rincón; A. López-Herrera; J.J. Echeverri
02/08/2013
Candidate migrant; Fall armyworm; Genetic structure; Insect resistance management; Molecular markers; SSRs

We developed six microsatellite markers for the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae). The SSR loci were isolated with enriched genomic library protocol by using native individuals as a genome source for markers. These loci were characterized in 48 individuals and they were tested for the ability to identify candidate migrants exchanged among the samples. ... more

V.A.C. Pavinato; S. Martinelli; P.F. de Lima; M.I. Zucchi; C. Omoto
02/04/2013
Allium sativum L.; Clones; Genetic divergence; Molecular markers

Outside its centers of origin, garlic propagates only asexually. Since asexual reproduction leads to the absence of meiotic recombination, the main garlic cultivars available for cultivation have arisen from the accumulation of somatic mutations in early cultivars. Thus, it is common for a single clone to have different names in different regions. This study aimed to evaluate the genetic ... more

R.G.F. Morales; J.T.V. Resende; F.V. Resende; C.A. Delatorre; A.S.T. Figueiredo; P.R. Da-Silva
01/07/2013
Cultivar identification; Molecular markers; Pennisetum glaucum; Seed piracy; SSR

The increasing number of protected and registered cultivars and problems involving seed commercialization make distinction and identification of cultivars imperative. Millet (Pennisetum glaucum), a crop species with protected cultivars in Brazil, has been the target of seed piracy. Thus, with the objective of identifying different lots with regard to origin, we characterized six ... more

R.P.Mendonça Neto; E.V.R. Von Pinho; B.L. Carvalho; G.S. Pereira
12/17/2012
Genetic diversity; Molecular markers; Morphological traits; Robinia pseudoacacia; Spaceflight

The black locust (Robinia pseudoacacia) is a forest legume that is highly valued as a honey plant and for its wood. We explored the effect of short-term spaceflight on development of R. pseudoacacia seedlings derived from seeds that endured a 15-day flight; the genetic diversity and variation of plants sampled from space-mutagenized seeds were compared to plants from ... more

C.Q. Yuan; Y.F. Li; P. Sun; Y.H. Sun; G.J. Zhang; M.S. Yang; Y.Y. Zhang; Y. Li; L. Wang
11/12/2012
Homology; Molecular markers; Monomorphic microsatellite; Silverleaf whitefly; Whitefly

The silverleaf whitefly (Bemisia tabaci) is actually a complex of morphologically indistinct species that vary in their capacity to transmit plant viruses and to induce physiological disorders in plants of economic importance. The worldwide impact of this whitefly has increased greatly, as it is a vector of various types of phytovirus, especially geminiviruses, in plants of ... more

G.E. Valle; A.L. Lourenção; M.I. Zucchi; J.B. Pinheiro
08/24/2012
Durable resistance; Molecular markers; Non-race-specific resistance; Slow-rusting; Triticum aestivum L.

Leaf rust is one of the most destructive diseases affecting wheat worldwide. The most effective way to control it is to use resistant cultivars. Resistance based on slow-rusting adult plant resistance (APR) genes has proven to be the best method for developing cultivars with durable resistance. A source of slow-rusting APR for leaf rust is the Brazilian wheat cultivar Toropi. The Toropi/ ... more

P.R. Da-Silva; S.P. Brammer; D. Guerra; S.C.K. Milach; A.L. Barcellos; M.I. Baggio
08/24/2012
454 Sequencing; Aquaculture; Bioinformatics; Haliotis midae; Molecular markers

Farming of Haliotis midae is the most lucrative aquaculture venture in South Africa. The genome of this species needs to be studied to assist in selective breeding programs aimed at increasing overall yield, and molecular markers will be required to attain this goal. We identified and characterized 82 polymorphic microsatellite loci by using repeat-enriched genomic libraries and ... more

R. Slabbert; J.A. Hepple; C. Rhode; A.E.Bester Van der Merwe; R. Roodt-Wilding
08/06/2012
EST-SSRs; Molecular markers; Musella; Polymorphism; Transferability

We assembled 31,308 publicly available Musa EST sequences into 21,129 unigenes; 4944 of them contained 5416 SSR motifs. In all, 238 unigenes flanking SSRs were randomly selected for primer design and then tested for amplification in Musella lasiocarpa. Seventy-eight primer pairs were found to be transferable to this species, and 49 displayed polymorphism. A set of 34 ... more

W.J. Li; H. Ma; Z.H. Li; Y.M. Wan; X.X. Liu; C.L. Zhou
08/06/2012
Cultivar identification; Molecular markers; RAPD; Sweet orange

We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar ... more

X. Sun; Q. Mu; D. Jiang; C. Wang; X.C. Wang; J.G. Fang

Pages