Research Article

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04/30/2015
Heading date; miRNA; Prediction model; Real-time PCR; Rice

Rice (Oryza sativa L.) is one of the most important food crops in the world. In Taiwan, due to the warm climate, there are two harvests annually. However, the yield and quality of rice can vary between each crop season in any given year. Previous reports have shown that microRNAs (miRNAs) play a crucial role in many developmental and physiological processes in plants. In this ... more

Y.C. Chen; W.S. Lin; R.K. Chen; Y.Y. Chao; S.W. Chin; F.C. Chen; C.Y. Lee
09/02/2004
Cysteine proteinase; Expression; Heterodera glycines; Inhibitory activity; Nematode; Pro domain peptide

Cysteine proteinases (CPs) are synthesized as zymogens and converted to mature proteinase forms by proteolytic cleavage and release of their pro domain peptides. A cDNA encoding a papain-like CP, called hgcp-Iv, was isolated from a Heterodera glycines J2 cDNA library, expressed and utilized to assess the ability of its propeptide to inhibit proteinase in its active ... more

F.B. Silva; J.A.N. Batista; B.M. Marra; R.R. Fragoso; A.Carolina S. Monteiro; E.L.Z. Figueira; M.Fátima Grossi-de-Sá
04/08/2008
Antigenic characterization; Cloning; Expression; GRA5; GRA7; ROP2; Toxoplasma gondii

Toxoplasma gondii is an intracellular obligate protozoan,which infects humans and warm-blooded animals. The aim of the present study was to clone the rop2, gra5 and gra7 genes from T. gondii RH strain and to produce recombinant proteins. The rop2, gra5 and gra7 gene fragments produced by polymerase chain reaction were ... more

M. Igarash; F. Kano; K. Tamekuni; P.M. Kawasaki; I.T. Navarro; O. Vidotto; M.C. Vidotto; R.Z. Machado; J.L. Garcia
11/22/2013
Cloning; Expression; Major histocompatibility complex; Stone flounder

Major histocompatibility complex (MHC) class II genes play important recognition roles in the immune system in vertebrates. We cloned the MHC class II genes A and B in the stone flounder (Kareius bicoloratus). The full-length cDNA and DNA sequences of both genes were obtained, and their characteristic motifs were analyzed. The DNA sequence of stone flounder MHC ... more

J. Jiang; C. Li; Q. Zhang; X. Wang
08/06/2012
Drosophila; Expression; GMR-GAL4; Wing

The GAL4/UAS binary system has been widely used in Drosophila melanogaster for ectopic expression of transgenes in a tissue-specific manner. The GMR-GAL4 driver, which expresses the yeast transcription factor GAL4 under the control of glass multiple reporter (GMR) promoter elements, has been commonly utilized to express target transgenes, specifically ... more

W.Z. Li; S.L. Li; H.Y. Zheng; S.P. Zhang; L. Xue
05/04/2015
Colon cancer; EpCAM; Wnt/β-catenin

The aims of this study were to explore the correlation between the expression of EpCAM and the Wnt/β-catenin pathway in human colon cancer and its clinical significance for the evaluation of cancer prognosis. Samples from colon cancer, para-carcinoma, or benign intestinal tissue from individual patients (50) and from normal intestinal mucosal tissues (20) were obtained from the Pathology ... more

F.Q. Zhou; Y.M. Qi; H. Xu; Q.Y. Wang; X.S. Gao; H.G. Guo
12/21/2015
BP neural network; Colon cancer; Gene expression; S-Kohonen neural network; SVM neural network

Based on gene expression, we have classified 53 colon cancer patients with UICC II into two groups: relapse and no relapse. Samples were taken from each patient, and gene information was extracted. Of the 53 samples examined, 500 genes were considered proper through analyses by S-Kohonen, BP, and SVM neural networks. Classification accuracy obtained by S-Kohonen neural network reaches 91 ... more

H.P. Hu; Z.J. Niu; Y.P. Bai; X.H. Tan
06/26/2015
Lacrimal gland carcinoma; RASSF1; Tumor suppressor gene

We examined the expression pattern of the tumor sup­pressor gene RAS association domain family gene 1 (RASSF1) in lacri­mal gland carcinoma and analyzed its relationship with the oncogenesis and progression of tumors. Sixty-two patients (30 males, 32 females, average age = 47 ± 3.5 years) admitted with lacrimal gland carcinoma to the Department of Ophthalmology of our hospital ... more

C.H. Zeng; B. Guo; J. Chen; W.M. He; Q.L. Luo

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