Research Article

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12/08/2011
Alternative splicing variant; DRD2 gene; Expression; Pig; Polymorphism

The dopamine D2 receptor (DRD2) is a crucial mediator for normal physiological processes. We cloned the pig DRD2 gene, investigated its distribution in tissues and identified polymorphisms by RT-PCR, quantitative real-time PCR and direct sequencing. Two Yorkshire pigs from Guangdong Academy of Agricultural Sciences (Guangzhou, China) were selected to clone the gene and ... more

H.P. Xu; X.M. He; M.X. Fang; Y.S. Hu; X.Z. Jia; Q.H. Nie; X.Q. Zhang
02/28/2013
Adipocytokines; Expression; Insulin resistance; Polycystic ovary syndrome; Subcutaneous adipose tissue

We reviewed emerging evidence linking serum levels and adipose tissue expression of leptin and adiponectin in women with polycystic ovary syndrome (PCOS). Previous data obtained by our group from a sample of overweight/obese PCOS women and a control sample of normal weight controls, both stratified by BMI, were reanalyzed. Circulating levels of leptin and adiponectin were determined by ... more

S.B. Lecke; D.M. Morsch; P.M. Spritzer
09/12/2013
Expression; Heterosis; MRLC2 gene; Sequence analysis; Tianfu goat

To determine the molecular basis of heterosis in goats, fluorescence quantitative polymerase chain reaction (PCR) was performed to investigate myosin-regulatory light chain 2 (MRLC2) gene expression in the longissimus dorsi muscle tissues of the Tianfu goat and its parents, the Boer and Chengdu Ma goats. The goat MRLC2 gene was differentially expressed in the crossbreed ... more

H.G. Xu; G.Y. Xu; L. Wan; J. Ma
09/18/2013
Alcoholism; Atp5g3; Epigenetic; Expression; Mouse; Transcriptome

The mitochondrial ATP synthase, subunit c, isoform 3 gene (Atp5g3) encodes subunit 9, the subunit of the multisubunit enzyme that catalyzes ATP synthesis during oxidative phosphorylation in mitochondria. According to the Ensembl database, Atp5g3 in mice is located on chromosome 2 between 73746504 and 73749383 bp, within the genomic regions of two sets of quantitative ... more

Y. Huang; L. Wang; B. Bennett; R.W. Williams; Y.J. Wang; W.K. Gu; Y. Jiao
02/27/2013
Chicken; Cloning; Expression; Nramp1 gene; Nramp2 gene; Polymorphism

Natural resistance-associated macrophage protein 1 and 2 encoding genes (Nramp1 and Nramp2) are related to many diseases. We cloned the cDNA of chicken Nramp1 and Nramp2 genes, characterized their expression and polymorphisms, and investigated the association of some SNPs with resistance to salmonellosis. The Nramp1 cDNA was 1746 bp long and the ... more

X.M. He; M.X. Fang; Z.T. Zhang; Y.S. Hu; X.Z. Jia; D.L. He; S.D. Liang; Q.H. Nie; X.Q. Zhang
12/19/2012
Chitin deacetylase gene; Chitinase-3 gene; Expression; Hyriopsis cumingii; Potential functions

Chitin, the second most important natural polymer in the world, and its N-deacetylated derivative chitosan are found in a wide variety of organisms. These versatile biopolymers are associated with a broad range of biological functions. This article is the first to report the potential functions of 2 chitin metabolic enzyme genes from Hyriopsis cumingii. A chitinase-3 gene ( ... more

G.L. Wang; B. Xu; Z.Y. Bai; J.L. Li
12/21/2012
Expression; Heat shock protein 110; Heat shock protein 110 messenger RNA; Heat stress; In vitro; Primary myocardial cells of rat

We investigated and described the kinetics of heat shock protein (Hsp) 110 expression and distribution in rat primary myocardial cells exposed to heat stress in vitro. After incubation at 37°C for 72 h, myocardial cells were heat stressed at 42°C for 0, 10, 20, 40, 60, 120, 240, 360, and 480 min. Significant increases in aspartate transaminase, lactate dehydrogenase, and ... more

Z.J. Liu; Y.J. Lv; M. Zhang; Z.H. Yue; S. Tang; A. Islam; B. Rehana; E.D. Bao; J. Hartung
11/28/2012
Ethylene receptor; Expression; Mangifera indica L.; Molecular modeling; Ripening

Ethylene induces characteristic ripening reactions in climacteric fruits through its binding to histidine-kinase (HK) receptors, activating the expression of ripening genes. Ethylene receptors have been found in Arabidopsis thaliana (Brassicaceae) and some fruits; number and expression patterns differ among species. In mango, only ethylene receptor ETR1 was known. We cloned ... more

C.A. Contreras-Vergara; N.A. Stephens-Camacho; G. Yepiz-Plascencia; G.A. González-Aguilar; A.A. Arvizu-Flores; E. Sanchez-Sanchez; M.A. Islas-Osuna
06/25/2012
Expression; FKBPs; Gene duplication; Maize

The receptors for the immunosuppression drugs FK506 and rapamycin are called FKBPs (FK506-binding proteins). FKBPs comprise a large family; they are found in many species, including bacteria, fungi, animals, and plants. As a class of peptidyl-prolyl cis-trans isomerase enzymes, the FKBP genes have been the focus of recent studies on plant stress tolerance and immunology. We ... more

W.W. Wang; Q. Ma; Y. Xiang; S.W. Zhu; B.J. Cheng
09/30/2004
Cancer; DmtDNA4977; In situ PCR; Kerns-Sayre syndrome deletion mutation; Mitochondria; Mitochondrial DNA; Real-time PCR

Levels of mtDNA4977 deletions (DmtDNA4977) have been found to be lower in tumors than in adjacent non-tumoral tissues. In 87 cancer patients, DmtDNA4977 was detected by multiplex polymerase chain reaction (PCR) amplification in 43 (49%) of the tumors and in 74 (85%) of the samples of non-tumoral tissues that ... more

M.Angela C. Dani; S.U. Dani; S.P.G. Lima; A. Martinez; B.M. Rossi; F. Soares; M.A. Zago; A.J.G. Simpson

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