Research Article

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07/25/2011
PCR-RFLP; Porcine stress syndrome gene; PSE; Swine

We evaluated the effect of the halothane (HAL) gene on the quality of pork in domestic pigs. Half-carcasses from two different commercial pig (Sus domestica) crossbreeds were analyzed, 46 of which were homozygous dominant (HALNN) and 69 of which were heterozygous (HALNn) for the halothane gene. The measures included backfat thickness, lean meat percentage, ... more

A.C.P. Silveira; P.F.A. Freitas; A.S.M. César; A.S.M. César; R.C. Antunes; E.C. Guimarães; D.F.A. Batista; L.C. Torido
01/30/2013
Intramuscular fat; Intron 1; L-FABP; Marbling; Single nucleotide polymorphism; Swine

This study was designed to investigate a single nucleotide polymorphism in intron 1 of the liver fatty acid-binding protein (L-FABP) gene in 156 Junmu No. 1 white swine using PCR-single-strand conformational polymorphism. The association between the polymorphism and meat quality traits was also studied. The cloning and sequencing results indicated that the polymorphism in intron ... more

Y.H. Zhang; L.S. Dai; T.H. Ma; S.Z. Wang; J. Guo; F.J. Li; S.M. Zhang; B.X. Sun; D.F. Liu; Y. Gao; J.B. Zhang
02/14/2014
Gene expression; Leptin; Leptin receptor; Swine

Leptin is an important regulator of appetite, energy metabolism, and reproduction and is mainly synthesized in the adipocytes and then secreted into the bloodstream. The leptin receptor was classified as type I cytokine receptor due to its structural homology with IL-6 receptors and the signaling pathways in which they are both involved. The aim of our study is to comparatively assess the ... more

S.E. Georgescu; M.A. Manea; S. Dinescu; M. Costache
12/12/2011
GHRH; Half-smooth tongue sole; Microsatellite polymorphism; mRNA expression; PACAP

Growth hormone-releasing hormone (GHRH) and pituitary adenylate cyclase activating polypeptide (PACAP) regulate development and somatic growth in teleosts; they may be associated with sexual growth dimorphism in the half-smooth tongue sole (Cynoglossus semilaevis). We found that the full-length GHRH and PACAP gene sequences obtained from females and males consist of 4160, 4159, ... more

Q. Ma; S.F. Liu; Z.M. Zhuang; L. Lin; Z.Z. Sun; C.L. Liu; Y.Q. Su; Q.S. Tang
08/29/2012
Bacterial agglutination; C-type Lectin; Epinephelus akaara; Genomic structure; mRNA expression; Recombinant expression

Because of their specific binding to carbohydrates, lectins play a crucial role in pathogen recognition and clearance in vertebrate animals. Previously, only two types of collectins had been isolated from bony fish: mannan-binding lectin (MBL) and galactose-binding lectin (GalBL). We sequenced a novel collectin (designated EALec1) from the red-spotted grouper, Epinephelus ... more

Z.W. Zhang; X.F. Shi; M. Qu; S.X. Ding
12/18/2014
Chitinase-like protein; Japanese scallop Mizuhopecten yessoensis; mRNA expression; Tissue degeneration

Chitinase-like proteins (CLP) are important members of the glycoside hydrolase family 18 (GH18) and are involved in growth control and remodeling processes. In this study, a CLP transcript was isolated and sequenced from the Japanese scallop (Mizuhopecten yessoensis) after screening expressed sequence tags. The full-length complementary DNA of M. yessoensis CLP ... more

L. Gao; G.J. Xu; H. Su; X.G. Gao; Y.F. Li; X.B. Bao; W.D. Liu; C.B. He
12/04/2014
Aeromonas hydrophila; APRIL; Aquareovirus; Grass carp; mRNA expression; TWEAK

TWEAK and APRIL are important members of the TNF superfamily, which play a crucial role in several diseases. Here, we describe the identification of grass carp (Ctenopharyngodon idella) homologs of TWEAK and APRIL (designated gcTWEAK and gcAPRIL, respectively) and their response to Aeromonas hydrophila and ... more

N.P. Pandit; Y.B. Shen; Y. Chen; W.J. Wang; J.L. Li
07/04/2014
3-glucan binding protein; Gene cloning; Hard clam; Lipopolysaccharide and β-1; Meretrix meretrix; mRNA expression

Pattern recognition molecules play an important role in innate immunity by recognizing conserved molecular patterns that are present on the surface of invading microorganisms. In this study, a lipopolysaccharide and β-1,3-glucan binding protein (LGBP) gene was cloned from the hard clam Meretrix meretrix (designated as Mm-LGBP) by the expressed sequence tags and ... more

S.X. Liu; Z.H. Qi; J.J. Zhang; C.B. He; X.G. Gao; H.J. Li
05/29/2015
Cold shock; Embryonic development; Gene cloning; mRNA expression; Sepiella maindroni (SmYB); Y-box

Y-box proteins are a family of highly conserved nucleic acid binding proteins that interact with genome and transcription product to modulate the transcriptional and translational processes. In the present study, a complete mRNA of Y-box binding protein (designated SmYB) was obtained from Sepiella maindroni by amplification of flanking sequences. The full size of SmYB cDNA was ... more

H.P. Si; C. .L.Wang; Y.Y. Zhang; C.K. Mu; P.P. Zhan; R.H. Li; W.W. Song
05/18/2015
Heat stress; Hsp60; mRNA expression; Oncorhynchus mykiss

The enhanced expression of heat shock proteins (hsps) in organisms can be detected in response to many kinds of stressor. For fish, high temperature is an important stressor, and hsp expression is associated with differences in environmental temperature. In this study, rainbow trout (Oncorhynchus mykiss) that were accustomed to an aquatic temperature of 18°C were exposed to an ... more

H.N. Shi; Z. Liu; J.P. Zhang; Y.J. Kang; J.F. Wang; J.Q. Huang; W.M. Wang

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