Research Article

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Allele frequency; DNA; Pool; Single nucleotide polymorphism

We examined the efficiency of direct sequencing of pooled DNA for developing common single nucleotide polymorphisms (SNPs) and its accuracy for estimating allele frequencies. A pool of 200 control DNAs was established and was used for developing SNPs and estimating minor allele frequencies (MAF). The sensitivity of the pooled DNA method for successfully detecting an SNP with an MAF >0. ... more

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Several lines of evidence suggest that the dopaminergic system is involved in the pathophysiology of major depressive disorder (MDD). Since the dopamine transporter (DAT1, also known as SLC6A3), mediates the active reuptake of dopamine from the synapses and thereby plays a vital role in the regulation of dopaminergic neurotransmission, we looked for a possible association between the C/T ... more

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Association study; Psoriasis; Psoriasis subtype; Serotonin 2A receptor; Single nucleotide polymorphism; Thai population

Expression of serotonin 2A receptor (5-HTR2A) is known to increase in psoriasis, a chronic inflammatory skin disease. We investigated a possible association between the -1438A/G single nucleotide polymorphism (rs6311) in the promoter region of 5-HTR2A gene and psoriasis in a Thai population. One hundred and twelve psoriatic patients and 151 unrelated healthy controls ... more

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Chinese Han population; Hypertension; Single nucleotide polymorphism

Human hypertension is a complex, multifactorial disease. Multiple variants associated with hypertension have been identified in the large numbers of genome-wide association studies, meta-analysis, and case-control studies. The present study investigated the association between the single nucleotide polymorphisms (SNPs) of five candidate genes and the susceptibility and prognosis of ... more

G.L. Hong; X.Z. Chen; Y. Liu; Y.H. Liu; X. Fu; S.B. Lin; Q. Zhu
Chinese sturgeon Acipenser sinensis; Hepatic lipase; Lipoprotein lipase; Mitochondrial control region; Performance on formulated diet; Single nucleotide polymorphism

It is very important to investigate the reasons for the large individual differences in individual performance of food acceptance when using formulated diets for the successful culture of larvae and juveniles of the Chinese sturgeon Acipenser sinensis. Genetic differences of the mitochondrial control region were investigated by direct sequencing in two groups of Chinese sturgeon ... more

Y. He; D. Shen; X.F. Liang; R.H. Lu; H. Xiao
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The purpose of this study was to determine whether interleukin-10 (IL-10) promoter polymorphisms are associated with leprosy or their subtypes in ethnic groups from southwest China. Genotyping using TaqMan® SNP Genotyping Master Mix and ABI 7500 real-time PCR system was performed for IL-10 T3575A, G2849A, C2763A, A1082G, C819T, and C592A in 189 healthy controls (40 ± 18 years) and 193 ... more

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Apoptosis; Cell cycle control; Childhood brain tumor; Single nucleotide polymorphism

We conducted a hospital-based case-control study in Korea to investigate whether apoptosis- and cell cycle control-related genes are associated with childhood brain tumor. Incident brain tumor cases (N = 70) and non-cancer controls (N = 140), frequency-matched by age and gender, were selected from 3 teaching hospitals in Seoul between 2003 and 2006. Tag single nucleotide polymorphisms ( ... more

S. Jeon; S. Han; K. Lee; J. Choi; S.K. Park; A.K. Park; H.S. Ahn; H.Y. Shin; H.J. Kang; H.H. Koo; J.J. Seo; J.E. Choi; H. Kim; Y. Ahn; D. Kang
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Vascular endothelial growth factor (VEGF), the most important regulator of angiogenesis and vascular permeability, is involved in various steps of carcinogenesis. The +936C/T polymorphism of the VEGF gene has been reported to affect the VEGF protein level and to be related to the susceptibility of cancer. However, the results of published studies, as well as the subsequent meta-analyses, ... more

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The renin-angiotensin-aldosterone system plays a key role in regulating blood pressure by maintaining vascular tone and the water/sodium balance. Many antihypertensive drugs target the renin-angiotensin-aldosterone system, but the effect differs considerably among hypertensive patients. We investigated whether genetic variants of the angiotensin II type 1 receptor are associated with ... more

H.T. Gong; X.L. Ma; B.X. Chen; X.Y. Xu; Q. Li; C.X. Guo; F.H. Du
High-resolution melting analysis; Lung cancer; Non-small cell lung cancer; Risk factor; Single nucleotide polymorphism

Although lung cancer (LC) is a highly environmentally associated disease, genetic factors are also thought to play a role in this disease. In recent years, genome-wide association studies have identified various susceptible genetic regions for LC. Herein, we used high-resolution melting analysis to genotype 2 significant single nucleotide polymorphisms previously reported in Caucasians, ... more

H. Wang; Y. Zhao; J. Ma; G. Zhang; Y. Mu; G. Qi; Z. Fang; L. Wang; Q. Fan; Z. Ma