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Related GMR Articles

09/04/2013
Myostatin; Promoter; Qinchuan cattle; Red Angus cattle; SNPs

To better understand the function of the myostatin gene and its promoter region in bovine, we amplified and sequenced the myostatin gene and promoter from the blood of Qinchuan and Red Angus cattle by using polymerase chain reaction. The sequences of Qinchuan and Red Angus cattle were compared with those of other cattle breeds available in GenBank. Exon splice sites were confirmed by mRNA ... more

Y.L. He; Y.H. Wu; F.S. Quan; Y.G. Liu; Y. Zhang
02/07/2013
Germin-like protein gene; GUS expression; Oryza sativa; Promoter; Tobacco transformation; Wounding

Germin and germin-like proteins (GLPs) are water-soluble extracellular proteins that are expressed in response to specific environmental and developmental signals. Although some enzymatic activities have also been associated with germin/GLPs, their role in overall metabolism is not fully understood. However, insight into their function may be gained by analysis of their promoter. The ... more

T. Mahmood; T. Yasmin; M.I. Haque; S.M.S. Naqvi
06/09/2014
5-bp indel (insertion and deletion); BMP7; Heteroduplex DNA; Promoter

Bone morphological protein7 (BMP7), a member of the transforming growth factor-β (TGF-β) family, was first identified because of its ability to induce ectopic chondro-osteogenesis in vivo. It also plays a crucial role in the growth, development, and physiological functioning of the reproductive system. Among intra-ovarian growth factors, many studies have shown that BMP7 plays a ... more

P. Lu; X. Feng; J.S. Zhou; X.B. Liu; Q.X. Chen; M. Li
02/28/2014
Asthma; IL-4; Meta-analysis; Polymorphisms; Promoter

This study aimed to more precisely assess the correlation between interleukin-4 (IL-4) promoter polymorphisms and the susceptibility risk of asthma. We conducted association studies on IL-4 promoter C-33T, C-589T, and G-1098T polymorphisms with asthma using data obtained from MEDLINE up to September 2011. Results showed that the polymorphisms IL-4 C-33T and C-589T were significantly ... more

L. Tang; H.G. Lin; B.F. Chen
02/27/2014
Chicken embryo; GFP; In vivo electroporation; Promoter

To investigate the variance of exogenous gene expression driven by different promoters by in vivo electroporation, 3 plasmid vectors carrying different promoters were selected, and their driving strength was compared in developing chicken embryos. The 3 promoters included: 1) the CAG promoter (containing the cytomegalovirus (CMV) immediate early enhancer and the chicken β-actin ... more

C.Q. Yang; X.Y. Li; Q. Li; S.L. Fu; H. Li; Z.K. Guo; J.T. Lin; S.T. Zhao
04/05/2011
Molecular cloning; Red drum; SoQM; Structure analysis

The QM-like gene encodes a ribosomal protein L10. Besides housekeeping roles in protein synthesis, QM-like proteins have multiple extraribosomal functions during cell growth, cell differentiation and apoptosis. We obtained the full-length cDNA of QM-like protein (designated as SoQM) from the salt water game fish Sciaenops ocellatus, using RACE-PCR. The sequence consists of 740 bp ... more

X. Chen; C.W. Wu; S.P. Zhong; F.R. Zeng; J.S. Zhang; J. Wang; S.F. Niu
03/06/2012
Molecular cloning; Penaeus monodon; Toll receptor

The black tiger shrimp (Penaeus monodon) is economically important in many parts of the world, including Thailand. Shrimp immunity is similar to that of other invertebrate organisms; it consists of an innate immunity system. Toll or Toll-like receptors (TLRs) play an essential role in recognizing the cleaved form of the cytokine Spätzle, which is processed by a series of ... more

W. Assavalapsakul; S. Panyim
11/19/2014
Bioactivity analysis; Giant panda; Interleukin 18; Molecular cloning

Interleukin 18 (IL-18), as a member of IL-1 superfamily, is an important pleiotropic cytokine that modulates Th1 immune responses. In this report, we cloned and identified a homolog of IL-18 in giant panda (Ailuropoda melanoleuca) (designated as AmIL-18) from peripheral blood mononuclear cells stimulated with lipopolysaccharide. The open readin g frame of AmIL-18 cDNA is ... more

Y. Yan; Q. Wang; L.L. Niu; J.B. Deng; J.Q. Yu; Y.Z. Zhang; J.X. Wang; M.M. Yin; X.M. Tan
11/25/2015
Cis-acting elements; Diterpenoids; Functional analysis; Hormone; Terpene lactones; Wounding

Ginkgolides are key pharmaceutical components in Ginkgo biloba. Using the cDNA sequence of the MECP and MECT genes to design primers, we obtained the promoters of these genes from Ginkgo genomic DNA using the genome walking method. The two promoters were 744 and 982 bp in length, respectively. The cis-elements of the GbMECPs and GbMECT promoters were predicted and ... more

S.Y. Cheng; L.L. Li; H.H. Yuan; F. Xu; H. Cheng

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