Research Article

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06/21/2011
Genetic diversity; Microsatellites; SSR markers; Wild sweet cherry

Wild sweet cherry (Prunus avium) trees are abundant in the northern part of Turkey, including the Coruh Valley. We analyzed 18 wild sweet cherry genotypes collected from diverse environments in the upper Coruh Valley in Turkey to determine genetic variation, using 10 SSR primers. These SSR primers generated 46 alleles; the number of alleles per primer ranged from 3 to 7, with a ... more

S. Ercisli; G. Agar; N. Yildirim; H. Karlidag; B. Duralija; A. Vokurka
07/19/2011
Frogs; Microsatellites; Physalaemus; Population structure; Related species

Various species of the Physalaemus cuvieri group of frogs are difficult to distinguish morphologically, making molecular analysis an attractive alternative for indentifying members of this group, which is considered to be at risk because of loss of habitat. The genetic structure of natural populations of P. ephippifer and P. albonotatus species was investigated ... more

M. Conte; M.I. Zucchi; G.V. Andrade; A.P. Souza; S.M. Recco-Pimentel
04/26/2011
CART gene; Goats; Microsatellites; Reproduction traits; SNPs

Polymorphisms of the CART gene were investigated by PCR-single-strand conformation polymorphism analysis in 540 samples from 10 goat breeds. Ten novel single-nucleotide polymorphisms as well as three microsatellites were detected; a mutation, 77T→C, led to an amino acid change (Leu→Ser). Associations between polymorphic loci and reproductive traits were analyzed in Chuandong White, ... more

P.Q. Wang; L.M. Deng; B.Y. Zhang; M.X. Chu; J.Z. Hou
08/03/2010
DNA; DYS464; Microsatellites; Population genetics; Worldwide populations; Y-chromosome

Of all DNA markers on the human Y-chromosome, the tetra-local Y-linked microsatellite DYS464 is the most polymorphic. We genotyped DYS464 in 677 male samples collected worldwide, maintained in the HGDP-CEPH Human Genome Diversity Cell Line Panel. Fourteen different alleles were found, with allele lengths varying from 9 to 23 repeats. One hundred and seventy-five ... more

F.S.G. Kehdy; S.D.J. Pena
01/05/2010
Comparative analysis of molecular groups; DNA markers; Microsatellites; RAPD and SSR loci; Zea mays

Using only one type of marker to quantify genetic diversity generates results that have been questioned in terms of reliability, when compared to the combined use of different markers. To compare the efficiency of the use of single versus multiple markers, we quantified genetic diversity among 10 S7 inbred popcorn lines using both RAPD and SSR markers, and we evaluated how well these two ... more

A.A. Leal; C.A. Mangolin; A.Tdo Amaral Júnior; L.S.A. Gonçalves; C.A. Scapim; A.S. Mott; I.B.O. Eloi; V. Cordovés; M.F.P. da Silva
02/09/2010
Electrophoretograms; Genetic diversity; Microsatellites; Molecular markers; Stutter peaks

Microsatellite markers are commonly used for examining population structure, especially inbreeding, outbreeding and gene flow. An array of microsatellite loci, preferably with multiallelic presentation, is preferable for ensuring accurate results. However, artifact peaks or stutters in the electrophoretograms significantly hamper the reliable interpretation of genotypes. We interpreted ... more

I.A. Arif; H.A. Khan; M. Shobrak; A.A.Al Homaidan; A. Sadoon; A.H.Al Farhan; A.H. Bahkali
08/12/2013
Alectrurus risora; Culicivora caudacuta; Microsatellites; Tyrannidae

Tyrant flycatchers (Aves: Tyrannidae) are endemic to the New World, and many species of this group are threatened or near-threatened at the global level. The aim of this study was to test the 18 microsatellite markers that have been published for other Tyrant flycatchers in the Strange-tailed Tyrant (Alectrurus risora) and the Sharp-tailed Tyrant (Culicivora caudacuta), ... more

B. Mahler; A.R.R. Schneider; A.S. Di Giacomo; A.G. Di Giacomo; J.C. Reboreda; R. Tiedemann
08/08/2013
Enriched library; Exopalaemon carinicauda; Microsatellites

The ridgetail white prawn Exopalaemon carinicauda is one of the major economic shrimp species cultured in China. In this study, 30 microsatellite loci were isolated and characterized for the ridgetail white prawn E. carinicauda using a microsatellite-enriched library. Polymorphisms were tested in 30 individuals from a single wild population. The number of alleles at ... more

S.W. Jia; P. Liu; J. Li; J.T. Li; L.Q. Pan
02/28/2013
Linkage analysis; Microsatellites; Mystus nemurus; Pseudo-testcross strategy; River catfish; Segregation

The river catfish Mystus nemurus is an important fresh water species for aquaculture in Malaysia. We report the first genetic linkage map of M. nemurus based on segregation analysis and a linkage map using newly developed microsatellite markers of M. nemurus. A total of 70 of the newly developed polymorphic DNA microsatellite markers were analyzed on pedigrees ... more

B.P. Hoh; S.S. Siraj; S.G. Tan; K. Yusoff
01/24/2013
454 Sequencing; Amphibians; Atlantic Forest; Euparkerella brasiliensis; Microsatellites

The new-generation 454 GS-FLX Titanium pyrosequencing was used to isolate microsatellite markers for the Brazilian Guanabara frog, Euparkerella brasiliensis, an Atlantic forest endemic species. Three multiplex polymerase chain reaction sets were optimized for genotyping of 11 polymorphic (di- and tetranucleotide) microsatellite markers. Genetic diversity was assessed in 21 ... more

L.A. Fusinatto; S. Lopes; A. Silva-Ferreira; J. Alexandrino; C.F.B. Haddad; C.F.D. Rocha; F. Sequeira

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