Research Article

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CG content; Hyperthermophiles; Microsatellites; Optimum growth temperature; SSRs; Thermophiles

All organisms that have been studied until now have been found to have differential distribution of simple sequence repeats (SSRs), with more SSRs in intergenic than in coding sequences. SSR distribution was investigated in Archaea genomes where complete chromosome sequences of 19 Archaea were analyzed with the program SPUTNIK to find di- to penta-nucleotide repeats. The number of ... more

S. Trivedi
03/22/2007
Brazil; Microsatellites; Mollusca; Mytilidae; Perna perna; Population genetics

Perna perna is the most important cultivated mussel of Santa Catarina, Brazil, sustaining an important economic input for many local families. Natural stocks of P. perna are depleted by the extraction of adults and seeds for consumption and culture. The aim of the present study was to use the microsatellite locus pms-2 to study the variation of the genetic ... more

K.T. Appio; L.I. Weber
07/13/2006
Ancestry; Microsatellites; Synthetic breed; Taurine; Zebu

The International Society of Animal Genetics (ISAG) has chosen nine microsatellites (international marker set) as a standard that should be included in all cattle parentage studies. They are BM1824, BM2113, INRA023, SPS115, TGLA122, TGLA126, TGLA227, ETH10, and ETH225. We decided to ascertain whether this microsatellite set could be used to determine ancestral proportions in ... more

H.M.S. Bicalho; C.G. Pimenta; I.K.P. Mendes; H.B. Pena; E.M. Queiroz; S.D.J. Pena
06/22/2006
matUPD15; Microsatellite analysis; Microsatellites; Prader-Willi syndrome

Prader-Willi syndrome (PWS) is a multisystemic disorder caused by the loss of expression of paternally transcribed genes in the PWS critical region of chromosome 15. Various molecular mechanisms are known to lead to PWS: deletion 15q11-q13 (75% of cases), maternal uniparental disomy (matUPD15) (23%) and imprinting defects (2%). FISH and microsatellite analysis are required to ... more

H.V. Aráoz; M. Torrado; C. Barreiro; L. Chertkoff
12/30/2005
Cebus apella; DNA polymorphisms; Microsatellites; Population structure; Short tandem repeats; Tufted capuchins

There have been numerous studies genetically characterizing Old World Primates using microsatellites. However, few studies have been made of New World species and none on free-ranging Cebus apella, even though it is probably the most widely distributed species of monkey in the New World. The paucity of studies is due, in part, to the lack of polymorphisms described for this species. We ... more

J.Margareth Amaral; A.Luiz Simões; D. De Jong
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Disease index; Leaf disc test; Microsatellites; Phytophthora palmivora; Quantitative trait loci; Theobroma cacao
E.S.L. Santos
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Genetic variability; Horse; Microsatellites; Pantaneiro horses

The genetic variability for a sample of 227 animals from three populations of Pantaneiro horses was estimated using data from 10 microsatellite loci. The number of alleles and the proportion of heterozygosity indicated high variability. A total of 91 alleles were found, with a significantly high mean number of alleles. The mean polymorphic information content was 0.7 and the paternity ... more

E.H. Giacomoni; G.P. Fernández-Stolz; T.R.O. Freitas
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Arapaima gigas; conservation genetics; Lower Amazon; Microsatellites; Multiplex polymerase chain reaction; pirarucu

The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtained demonstrate high heterozygosity ( ... more

I.G. Hamoy; E.J.M. Santos; S.E.B. Santos
12/21/2011
Aquaculture; Babylonia areolata; Microsatellites; Population genetics; Spotted babylon

The spotted babylon, Babylonia areolata, is one of the most extensively cultured marine mollusks in southeast Asia. Eight polymorphic microsatellite markers were developed for this species, from a microsatellite-enriched library. These markers, characterized in 32 individuals from a hatchery population, were polymorphic, with allele numbers ranging from 6 to 18 per ... more

Y. Wang; H. Lu; J. Zheng; K. Long; Y.H. Shi; Z.F. Gu; A.M. Wang
10/07/2011
Horse; Microsatellites; South America; Venezuelan Criollo

Various horse populations in the Americas have an origin in Spain; they are remnants of the first livestock introduced to the continent early in the colonial period (16th and 17th centuries). We evaluated genetic variability within the Venezuelan Criollo horse and its relationship with other horse breeds. We observed high levels of genetic diversity within the Criollo breed. Significant ... more

E.G. Cothran; J.L. Canelon; C. Luis; E. Conant; R. Juras

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