Research Article

Related GMR Articles

01/16/2015
DNA; Extraction; Mastitis; Real-time polymerase chain reaction; Staphylococcus aureus

The aim of this study was to evaluate the repeatability and performance of 4 methods of extracting DNA from Staphylococcus aureus (SAU) and the gene encoding bovine mitochondrial cytochrome B (BMCB) in milk samples from cows with subclinical mastitis for use in amplification by real-time polymerase chain reaction. Two milk samples were obtained from cows naturally infected with ... more

A.G. Dibbern; B.G. Botaro; M.P. Viziack; L.F.P. Silva; M.V. Santos
03/30/2007
Brangus; Carcass; DNA; Growth; Growth hormone; SNP

Sequence polymorphisms in the growth hormone (GH) gene and its transcriptional regulators, Pit-1 and Prop-1, were evaluated for associations with growth and carcass traits in two populations of Brangus bulls Chihuahuan Desert Rangeland Research Center (CDRRC, N = 248 from 14 sires) and a cooperating breeding program (COOP, N = 186 from 34 sires). Polymorphisms were SNP mutations in intron 4 (C ... more

M.G. Thomas; R.M. Enns; K.L. Shirley; M.D. Garcia; A.J. Garrett; G.A. Silver
03/15/2007
Acridine orange test; Bovine; Conservation; DNA; Freeze-drying sperm; TUNEL

The ability to detect nuclear damage is an important tool for the development of sperm preservation methods. We used the acridine orange test (AOT) and the terminal deoxynucleotidyl transferase-mediated dUDP nick-end labeling (TUNEL) assay to assess the DNA status of sperm cells preserved with different lyophilization media. The AOT did not detect any differences between different ... more

C.F. Martins; M.N. Dode; S.N. Báo; R. Rumpf
03/10/2009
Algorithm; Bayesian networks; DNA; Paternity testing; Probabilistic expert systems; Software

PedExpert is a Windows-based Bayesian network software, especially constructed to solve problems in parentage testing that are complex because of missing genetic information on the alleged father and/or because they involve genetic mutations. PedExpert automates the creation and manipulation of Bayesian networks, implementing algorithms that convert pedigrees and sets of indispensable ... more

R.R. Gomes; S.V.A. Campos; S.D.J. Pena
04/27/2010
Allele frequency; DNA; Pool; Single nucleotide polymorphism

We examined the efficiency of direct sequencing of pooled DNA for developing common single nucleotide polymorphisms (SNPs) and its accuracy for estimating allele frequencies. A pool of 200 control DNAs was established and was used for developing SNPs and estimating minor allele frequencies (MAF). The sensitivity of the pooled DNA method for successfully detecting an SNP with an MAF >0. ... more

M.K. Kim; T.S. Nam; K.H. Choi; S.Y. Jang; Y.O. Kim; M.C. Lee
03/31/2015
DNA; Mesquite; Mexicali valley; Microorganism; Phosphorous

One of the main limitations in intensive crop production in Northwestern Mexico is the dependence on the use of phosphate fertilizer. In this study, we isolated indigenous microorganisms with phosphate solubilization capacities from mesquite (Prosopis glandulosa) present in the Mexicali valley. In total, 4 bacteria were isolated from the rhizosphere of mesquite, including ICA01, ... more

L. Moreno-Ramírez; D. González-Mendoza; C. Cecena-Duran; O. Grimaldo-Juarez
05/12/2015
Germination; Real-time PCR; Seed quality; Seed vigor

Phytohormones have different characteristics and functions, and they may be subject to changes in their gene expression and synthesis during seed development. In this study, we evaluated the physiological qualities of habanero peppers (Capsicum chinense Jacquin) during seed development and the expression of genes involved in germination. Seeds were obtained from fruits harvested ... more

H.O. Santos; E.V.R. Von Pinho; I.V. Von Pinho; S.M.F. Dutra; T. Andrade; R.M. Guimarães
03/30/2015
Breeding; Cucumber; Germination; Mutation; Seedlings; Weight

We attempted to create a new germplasm of cucumber cultivar Chinese long (9930) using different doses of ethyl methyl sulfonate (EMS) to induce variability. We tested EMS concentration (0, 0.5, 1.0, 1.5, 2, 3% v/v) with post-treatment (0.1 M Na2S2O3 and water), EMS concentration (0, 0.5, 1.0, 1.5% v/v) over different treatment times (8, 16, 24 h), and EMS ... more

S.N.M. Shah; Z.H. Gong; M.H. Arisha; A. Khan; S.L. Tian
10/30/2018
Isozyme markers; Nicotiana tabacum; Physiological maturity; Vigor

Morphological or isozyme markers related to physiological maturation and deteriorative processes are important in the evaluation of seed quality. Two experiments were conducted to examine the possibility of using isozymes as indicators of quality in tobacco seed lots and fruit appearance as an indicator of physiological maturity in tobacco cultivars, based on the physiological and biochemical ... more

D.B. Andrade; H.P. Silva; M.L.M. Carvalho; A.S. Oliveira; H.O. Santos; I.C.Silva Neta
02/25/2019
Assisted selection; DNA extraction; DNA marker; Viability; Vigor

DNA-assisted selection can be applied to vegetal species in the seed stage; however, little is known about the effect of seed fractionation on the physiological quality and viability of the seedlings or the effectiveness of DNA extraction from seed pieces. We evaluated the efficiency of pre-germinative genotypic screening by DNA markers from manually cut partial ... more

R.R. de Brito; E.F. Leão-Araújo; L.Bda S. Ferreira; deM.O. Mendes; C.Cde O. Pereira; J.O. da Silva; E. Bógea; L.V. Hoffmann; I.P.P. de Menezes

Pages