Research Article

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Chromatin; DNA methylation; FISH; Micrococcal nuclease; Myostatin

Myostatin is a negative regulator of the growth and development of skeletal muscle mass. In fish, myostatin is expressed in several organs in addition to skeletal muscle. To understand the mechanisms regulating myostatin gene expression in the sea perch, Lateolabrax japonicus, we examined the methylation status of the myostatin gene promoter region in several tissues (liver, eye, kidney, brain ... more

E.M. Abbas; A. Takayanagi; N. Shimizu; M. Kato
07/08/2013
DNA methylation; Epigenetic inheritance; Molecular marker; Mutation analysis; Plant breeding

Fig (Ficus carica) breeding programs that use conventional approaches to develop new cultivars are rare, owing to limited genetic variability and the difficulty in obtaining plants via gamete fusion. Cytosine methylation in plants leads to gene repression, thereby affecting transcription without changing the DNA sequence. Previous studies using random amplification of polymorphic ... more

M.G.F. Rodrigues; A.B.G. Martins; B.W. Bertoni; A. Figueira; S. Giuliatti
04/17/2013
DNA methylation; Osmotic stress; Proline; Rice

Proline accumulation is involved in plant osmotic stress tolerance. Given that DNA methylation is related to almost all metabolic processes through regulation of gene expression, we suspected that this epigenetic modification and proline biosynthesis are probably related. To test this, we investigated whether osmotic stress-induced proline accumulation is associated with DNA methylation ... more

C.Y. Zhang; N.N. Wang; Y.H. Zhang; Q.Z. Feng; C.W. Yang; B. Liu
09/26/2012
DNA methylation; Fat; MSAP; Muscle; Pig

DNA methylation may be involved in regulating the expression of protein-coding genes, resulting in different fat and muscle phenotypes. Using a methylation-sensitive amplified polymorphism approach, we obtained 7423 bands by selective amplification of genomic DNA from six different fat depots and two heterogeneous muscle types from Duroc/Landrace/Yorkshire cross-bred pigs. The degrees of ... more

J.D. Ma; M.Z. Li; S.L. Zhou; C.W. Zhou; X.W. Li
08/29/2012
DNA methylation; Gastric cancer; LOH; MSI; PAI-1 gene

We explored a possible correlation of genetic instability and CpG methylation in the 5'-flanking region of the PAI-1 gene with clinicopathologic features of gastric cancer in Chinese patients and looked for molecular markers for diagnosing gastric tumor development. Microsatellite instability and loss of heterozygosity of the PAI-1 gene locus D7S515, D7S471 and pai-1 ... more

J. Liu; X. Li; N. Yu; Y.Q. Yang; X. Li; Z.Y. Ye; J.C. Li
02/16/2012
DNA methylation; Histone acetylation; PEV; Transgene expression; Transgenic pig

Sequences proximal to transgene integration sites are able to regulate transgene expression, resulting in complex position effect variegation. Position effect variegation can cause differences in epigenetic modifications, such as DNA methylation and histone acetylation. However, it is not known which factor, position effect or epigenetic modification, plays a more important role in the ... more

Z. Yin; Q.R. Kong; Z.P. Zhao; M.L. Wu; Y.S. Mu; K. Hu; Z.H. Liu
10/20/2014
DNA methylation; Heterosis; Hybrid; Maize; Methylation-sensitive amplification polymorphism

Heterosis is the superior performance of heterozygous individuals and has been widely exploited in plant breeding, although the underlying regulatory mechanisms still remain largely elusive. To understand the molecular basis of heterosis in maize, in this study, roots and leaves at the seedling stage and embryos and endosperm tissues 15 days after fertilization of 2 elite hybrids and ... more

T.J. Liu; L.F. Sun; X.H. Shan; Y. Wu; S.Z. Su; S.P. Li; H.K. Liu; J.Y. Han; Y.P. Yuan
07/07/2014
DNA methylation; DNA methyltransferases; Osmotic stress; Rice; Salt stress

Cytosine DNA methylation is a conserved epigenetic regulatory mechanism in both plants and animals. DNA methyltransferases (DNA MTases) not only initiate (de novo) but also maintain the process of DNA methylation. Here, we characterized the genome-wide expression profiles of 10 cytosine DNA MTase genes belonging to 4 subfamilies, MET1, CMT, DNMT2, and ... more

F. Ahmad; X. Huang; H.X. Lan; T. Huma; Y.M. Bao; J. Huang; H.S. Zhang
04/29/2014
CD4; DNA methylation; EMSA; NF-κB; Porcine kidney epithelial cells

The effects of virus-like double-stranded RNA (dsRNA, PolyI:C) and DNA methyltransferase inhibitor (Aza-CdR) on CD4 gene expression were investigated in a porcine kidney cell line (PK15). We found that expression levels of TLR3 and IFNαwere significantly upregulated by PolyI:C, compared to the untreated PK15 cells, which shows that PolyI:C successfully ... more

X.S. Wang; H. Ao; L.W. Zhai; L.J. Bai; W.Y. He; C.D. Wang; Y. Yu
05/04/2015
Cancer antigen 125; Cationic conjugated polymer-based fluorescence resonance energy transfer; DNA methylation; Epithelial ovarian cancer; Methylation level detection

DNA methylation plays an important role in carcinogenesis and cancer development. In this study, we examined gene methylation in epithelial ovarian cancer (EOC) using cationic conjugated polymer (CCP)-based fluorescence resonance energy transfer (FRET) to evaluate the application of cumulative methylation alternations of genes associated with cancer antigen 125 for early cancer diagnosis ... more

B.L. Xing; T. Li; Z.H. Tang; L. Jiao; S.M. Ge; X. Qiang; J. OuYang

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