Research Article

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Radish floral bud abortion (FBA) is an adverse biological phenomenon that occurs during reproduction. Although FBA occurs frequently, its mechanism remains unknown. To elucidate the molecular mechanism underlying FBA, we detected gene expression differences between aborted and normal buds of radish using cDNA-amplified fragment length polymorphism (AFLP) and real-time polymerase chain ... more

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BPI gene; Gene expression; Pig; Real-time PCR

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J. Zhu; C. Zi; Z.C. Wu; L. Liu; X.R. Zheng; X.M. Su; G.Q. Zhu; X.G. Huang; S.L. Wu; W.B. Bao
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Colorectal cancer; DNA hypomethylation; Epigenetics; Gene expression; Metastasis

DNA methylation is a fundamental epigenetic mechanism in regulating the expression of genes controlling crucial cell functions in cancer development. Gene silencing via CpG island methylation/demethylation in the promoter region is one of the mechanisms by which different genes are inactivated/activated in human cancers. Tissue inhibitor of metalloproteinase-2 (TIMP-2) is known to ... more

Z. Hussain; M.I. Khan; M. Shahid; F.N. Almajhdi
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Phosphatidylinositol phosphate kinases (PIPKs) are enzymes that participate in diverse intracellular signaling pathways. They are classified into 3 functionally distinct subfamilies - PIPKI (a, b, g), PIPKII (a, b, g), and PIPKIII - located in various subcellular compartments. Recently, the PIPKIIa and b-globin genes were found to be overexpressed in reticulocytes from 2 siblings with ... more

T.R. Zaccariotto; C. Lanaro; D.M. Albuquerque; M.N.N. Santos; M.A.C. Bezerra; F.G.P. Cunha; I. Lorand-Metze; A.S. Araujo; F.F. Costa; M.F. Sonati
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Dithranol; Gene expression; HaCaT; Id1; Psoriasis

The precise causes of psoriasis, a chronic skin disorder characterized by hyperproliferation of keratinocytes and incomplete keratinization, are unclear. It is known that expression of helix-loop-helix transcription factor Id1, which functions as an inhibitor of differentiation, is upregulated in psoriatic skin. We investigated the effect of the antipsoriatic drug dithranol on mRNA and ... more

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Coffee; Gene expression; Maturation; PME; Quality

Coffee quality is directly related to the harvest and post harvest conditions. Non-uniform maturation of coffee fruits, combined with inadequate harvest, negatively affects the final quality of the product. Pectin methylesterase (PME) plays an important role in fruit softening due to the hydrolysis of methylester groups in cell wall pectins. In order to characterize the changes occurring ... more

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Gene expression; Heat shock protein 90; heavy metal; Phascolosoma esculenta; Western blot

The full-length complementary DNA (cDNA) of heat shock protein 90 was cloned from Phascolosoma esculenta (PeHSP90) using expressed sequence tag and rapid amplification of cDNA end approaches. The cDNA of PeHSP90 was 2521 bp including a 5'-untranslated region of 110 bp, a 3'-untranslated region of 230 bp, and an open reading frame of 2181 bp. All of the characteristic motifs of ... more

C.H. Li; X.R. Su; M.Q. Wang; Y.Y. Li; Y. Li; J. Zhou; T.W. Li
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Gene expression; HSP104; Protein structure; Trypanosoma cruzi

Heat shock protein (HSP) 104 is a highly conserved molecular chaperone that catalyzes protein unfolding, disaggregation and degradation under stress conditions. We characterized HSP104 gene structure and expression in Trypanosoma cruzi, a protozoan parasite that causes Chagas’ disease. The T. cruzi HSP104 is an 869 amino-acid protein encoded by a single-copy ... more

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Cerebral ischemia or ischemic stroke is mainly attributed to vascular and circulation disorders. Among protein biomarkers, RNA profiles have also been identified as markers of ischemic stroke. MicroRNA-145 expression is ostensibly recognized as marker and modulator of vascular smooth muscle cell phenotype; however, expression levels in ischemic stroke had not been investigated. Employing ... more

C.S. Gan; C.W. Wang; K.S. Tan

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