Research Article

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10/31/2011
Cloning; Gene expression; Granulocyte colony-stimulating factor; Recombinant G-CSF; Synthetic cDNA

Recently, granulocyte colony-stimulating factor (G-CSF) has been recognized as a useful molecule for the treatment of a wide range of complex ailments, such as cancer, AIDS, H1N1 influenza, cardiac and neurological diseases. The vast therapeutic potential of G-CSF has induced scientists to develop biotechnological approaches for the synthesis of this pharmacologically active agent. We ... more

S. Alrokayan
08/05/2011
cDNA; Cloning; Genomic sequence; Giant panda (Ailuropoda melanoleuca); Overexpression; Ribosomal protein gene L9 (rpL9)

The ribosomal protein L9 (RPL9), a component of the large subunit of the ribosome, has an unusual structure, comprising two compact globular domains connected by an α-helix; it interacts with 23 S rRNA. To obtain information about rpL9 of Ailuropoda melanoleuca (the giant panda) we designed primers based on the known mammalian nucleotide sequence. RT-PCR and PCR ... more

W.R. Hou; Y.L. Hou; G.F. Wu; Y. Song; X.L. Su; B. Sun; J. Li
12/14/2010
Cloning; Litopenaeus vannamei; Ryanodine receptor; Single-nucleotide polymorphism

Ryanodine receptor/calcium release channel is a large protein that plays an essential role in muscle contraction; mutations in the ryanodine receptor gene affect sensitivity to stress. As a first step towards investigating the relationship between the ryanodine receptor and shrimp cramped muscle syndrome, we cloned, partially sequenced, and examined single-nucleotide polymorphisms (SNPs) ... more

X.H. Chen; D.G. Zeng; N. Ma
11/22/2013
Cloning; Expression; Major histocompatibility complex; Stone flounder

Major histocompatibility complex (MHC) class II genes play important recognition roles in the immune system in vertebrates. We cloned the MHC class II genes A and B in the stone flounder (Kareius bicoloratus). The full-length cDNA and DNA sequences of both genes were obtained, and their characteristic motifs were analyzed. The DNA sequence of stone flounder MHC ... more

J. Jiang; C. Li; Q. Zhang; X. Wang
11/22/2013
Cloning; Litopenaeus vannamei; Macrophage migration inhibitory factor

The macrophage migration inhibitory factor (MIF) is an important proinflammatory cytokine that mediates both innate and adaptive immune responses. In this study, we identified a homolog of MIF in the Pacific white shrimp Litopenaeus vannamei. The MIF cDNA contained a 363-bp open reading frame encoding a 120-amino acid protein with a calculated molecular mass of 13.442 kDa and a theoretical ... more

D.G. Zeng; A.Y. Lei; X.H. Chen
10/18/2013
Anticancer activity; cDNA; Cloning; Giant panda; Overexpression; Purification; Ribosomal protein L24

The ribosomal protein L24 (RPL24) belongs to the L24E family of ribosomal proteins and is located in the cytoplasm. The purpose of this study was to investigate the structure and anti-cancer function of RPL24 of the giant panda (Ailuropoda melanoleuca). The complementary DNA of RPL24 was cloned successfully using reverse transcription-polymerase chain reaction technology ... more

Y.L. Hou; X. Ding; W. Hou; B. Song; T. Wang; F. Wang; J. Li; J. Zhong; T. Xu; B.X. Ma; H.Q. Zhu; J.H. Li; J.C. Zhong
10/07/2013
Bioinformatic analysis; Cloning; Molecular characterization; Pseudorabies virus; UDG; UL2

A 948-bp sequence of the UL2 gene was amplified from the pseudorabies virus (PRV) Becker strain genome using polymerase chain reaction, and the gene identity was confirmed through further cloning and sequencing. Bioinformatic analysis indicated that the PRV UL2 gene encodes a putative polypeptide with 315-amino acid residues. Its encoding protein, designated UL2, has a ... more

M.S. Cai; B.Y. Wang; W. Cui; Z.Y. Zhao; J.H. Chen; X.M. Wen; Z. Li; M.L. Li
02/27/2013
Chicken; Cloning; Expression; Nramp1 gene; Nramp2 gene; Polymorphism

Natural resistance-associated macrophage protein 1 and 2 encoding genes (Nramp1 and Nramp2) are related to many diseases. We cloned the cDNA of chicken Nramp1 and Nramp2 genes, characterized their expression and polymorphisms, and investigated the association of some SNPs with resistance to salmonellosis. The Nramp1 cDNA was 1746 bp long and the ... more

X.M. He; M.X. Fang; Z.T. Zhang; Y.S. Hu; X.Z. Jia; D.L. He; S.D. Liang; Q.H. Nie; X.Q. Zhang
01/22/2013
Bioinformatics; Cloning; ICP22; Molecular characterization; Pseudorabies virus; US1

Using polymerase chain reaction, a 1050-bp sequence of the US1 gene was amplified from the pseudorabies virus (PRV) Becker strain genome; identification of the US1 gene was confirmed by further cloning and sequencing. Bioinformatics analysis indicated that the PRV US1 gene encodes a putative polypeptide with 349 amino acids. The encoded protein, designated ... more

M.L. Li; J.H. Chen; Z.Y. Zhao; K.J. Zhang; Z. Li; J. Li; J.Y. Mai; X.M. Zhu; M.S. Cai
08/29/2012
Capsicum annuum L.; Cloning; Cytoplasmic male sterility; NADP-isocitrate dehydrogenase; Sequence characterization

NADP-dependent isocitrate dehydrogenase (NADP-ICDH) is an important enzyme involved in energy metabolism. The complete coding sequence of the pepper (Capsicum annuum) NADP-ICDH gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs. ... more

M.H. Deng; J.F. Wen; J.L. Huo; H.S. Zhu; X.Z. Dai; Z.Q. Zhang; H. Zhou; X.X. Zou

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