Research Article

Related GMR Articles

12/16/2013
Allelic frequency; Genetic polymorphisms; Hemoglobin S; PCR-RFLP; Sickle cell disease; SNPs

Sickle cell anemia is an affection that causes chronic inflammation, with consequences for vaso-occlusion, oxidative stress and cytokine production. Genetic polymorphisms in markers involved in this process can modulate the inflammatory response, including polymorphisms -308G/A of TNFA (tumor necrosis factor alpha) and -509C/T of TGFB1 (transforming growth factor beta 1), reported to ... more

L.S. Torres; B. Júnior; D.G. Silva; C.L. Lobo; M.A. Ruiz; C.R. Bonini-Domingos
02/28/2013
Body measurement traits; DKK4 gene; PCR-SSCP; Qingchuan cattle; SNPs

The aim of this study was to determine whether single nucleotide polymorphisms (SNPs) of bovine Dickkopf homolog 4 (DKK4) are associated with body measurement traits in Qinchuan cattle. By using PCR-SSCP technology and DNA sequencing, we discovered 5 DKK4 SNPs in Qingchuan cattle, including -65G>A and -77G>T in the 5ꞌ-untranslated region, 1532C>G and 1533T>C ... more

J.B. Gao; Y.K. Li; N. Yang; X.H. Ma; C. Adoligbe; B.J. Jiang; C.Z. Fu; G. Cheng; L.S. Zan
12/06/2013
CTLA-4 gene; Diabetes; Meta-analysis; SNPs

The aim of this study was to evaluate the association between the rs3087243 polymorphism of the cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) gene and the risk of type 1 diabetes mellitus (T1D). A comprehensive meta-analysis of case-control studies was conducted to determine the association between the rs3087243 polymorphism (CT60A/G) and T1D and assess the joint evidence ... more

J. Liu; H.X. Zhang
09/23/2013
CTLA-4 gene; Diabetes; Meta-analysis; SNPs

We evaluated association of polymorphisms in the CTLA-4 gene with the risk of type 1 diabetes mellitus. Comprehensive meta-analysis was applied to case-control studies of the association between CTLA-4 and type 1 diabetes mellitus to assess the joint evidence for the association, the influence of individual studies, and evidence for publication bias. We searched PubMed ... more

J. Liu; H.X. Zhang; G.Y. Feng; L. He
09/04/2013
Myostatin; Promoter; Qinchuan cattle; Red Angus cattle; SNPs

To better understand the function of the myostatin gene and its promoter region in bovine, we amplified and sequenced the myostatin gene and promoter from the blood of Qinchuan and Red Angus cattle by using polymerase chain reaction. The sequences of Qinchuan and Red Angus cattle were compared with those of other cattle breeds available in GenBank. Exon splice sites were confirmed by mRNA ... more

Y.L. He; Y.H. Wu; F.S. Quan; Y.G. Liu; Y. Zhang
07/30/2013
Chinese native cattle; CXCR1; Milk traits; SCS; SNPs

Mastitis is an economically devastating disease affecting the dairy industry. Dairy cows with mastitis give reduced milk yield and produce milk that is unfit for consumption. The chemokine receptor CXCR1 is an excellent prospective genetic marker for mastitis resistance in cattle because it regulates neutrophil migration, killing, and survival during infection. We detected 4 single ... more

L. Zhou; H.M. Wang; Z.H. Ju; Y. Zhang; J.M. Huang; C. Qi; M.H. Hou; L.G. An; J.F. Zhong; C.F. Wang
01/24/2013
Crohn’s disease; Malaysia; SNPs; STAT3; TYK2

This study aimed to investigate the potential association of TYK2 and STAT3 genes with the susceptibility to Crohn’s disease (CD) among Malaysians. DNA samples were obtained from 80 CD patients and 100 healthy controls. Polymerase chain reaction-restriction fragment length polymorphism methods were employed for genotyping, followed by statistical analysis. In our current ... more

L.H. Lian; T.P. Lau; V.L. Lee; W.S. Lee; I. Hilmi; K.L. Goh; K.H. Chua
10/15/2012
Bovine; MBL2; SNPs; Somatic cell score

The mannan-binding lectin gene (MBL) participates as an opsonin in the innate immune system of mammals, and single nucleotide polymorphisms (SNPs) in MBL cause various immune dysfunctions. In this study, we detected SNPs in MBL2 at exon 1 using polymerase chain reaction single-strand conformation polymorphism analysis and DNA sequencing techniques in 825 Chinese ... more

Z.L. Zhao; C.F. Wang; Q.L. Li; Z.H. Ju; J.M. Huang; J.B. Li; J.F. Zhong; J.B. Zhang
10/19/2012
Meat traits; Molecular marker; PPAR-γ gene; SNPs

The peroxisome proliferator-activated receptor gamma (PPAR-γ) is a key molecule in adipocyte differentitation; it transactivates multiple target genes in lipid metabolic pathways. Using PCR-SSCP and DNA sequencing, we evaluated a potential association of an SNP (72472 G﹥T in exon7) of the bovine PPAR-γgene with carcass and meat quality traits in 660 ... more

Y.Y. Fan; G.W. Fu; C.Z. Fu; L.S. Zan; W.Q. Tian
09/25/2012
CD14; Chinese Han; SNPs; Tuberculosis

Although the role of CD14 in mediating signals from Toll-like receptors to recognize Mycobacterium tuberculosis is known, how polymorphisms in this gene affect the susceptibility to develop tuberculosis are still not clear. We examined whether single nucleotide polymorphisms at positions -1145 and -159 in the promoter region of the CD14 gene are associated with tuberculosis in a ... more

M.Y. Zhao; Y. Xue; Z.Q. Zhao; F.J. Li; D.P. Fan; L.L. Wei; X.J. Sun; X. Zhang; X.C. Wang; Y.X. Zhang; J.C. Li

Pages