Research Article

Related GMR Articles

Adipose tissue; Conjugated linoleic acid; Gene expression; MicroRNA; Pig

In this study, we evaluated the effect and possible mech­anism of action of dietary conjugated linoleic acid (CLA) on pig body fat deposition. Landrace piglets (N = 48) were randomly divided into three groups, which were fed diets containing 0% (control), 1%, or 2% CLA. Dorsal and abdominal subcutaneous adipose tissues were col­lected, and real-time polymerase chain reaction (PCR) was ... more

R.L. Qi; Y. Chen; J.X. Huang; F.Y. Yang
Adipose tissue; Comet assay; Mesenchymal stem cells

The use of mesenchymal stem cells (MSCs) in experimental, clinical, and therapeutic trials has grown in recent years. However, the issue remains of whether these procedures are completely safe for transplant patients. Therefore, this study was designed and carried out with the aim of evaluating two different comet assay protocols for genomic damage pattern analysis in MSCs derived from ... more

L.C. Hermeto; R.J. Oliveira; R. Matuo; P.H.A. Jardim; R. DeRossi; A.C.M.B. Antoniolli; E. Deffune; T.C. Evaristo; Á.E. Santana
Adipose tissue; Buffalo; Cattle; Cell therapy; Mesenchymal stem cells

Adult stem cells are known for their plasticity and their potential to differentiate into several different cell types; these characteristics have implications for cell therapy and reproductive biotechnologies. In this study, we report on the isolation and characterization of mesenchymal stem cells (MSC) derived from bovine and buffalo adipose tissue. Cells isolated using enzymatic ... more

R.V. Sampaio; M.R. Chiaratti; D.C.N. Santos; F.F. Bressan; J.R. Sangalli; A.L.A. Sá; T.V.G. Silva; N.N. Costa; M.S. Cordeiro; S.S.D. Santos; C.E. Ambrósio; P.R. Adona; F.V. Meirelles; M.S. Miranda; O.M. Ohashi
ChaT; Ischemia/reperfusion; Liver; Passive avoidance test; Postoperative cognitive dysfunction

The aim of this study was to investigate the effects of partial hepatic ischemia/reperfusion (I/R) on postoperative cognitive function in mice. One hundred Kunming mice were randomized into control group (N = 20), sham group (N = 20) and I/R group (N = 60), which was equally divided into 3 subgroups according to the ischemia time (20, 30 and 40 min). Half of the mice in each group ... more

Y.Q. Wang; W.W. Wu; L.K. Wang; K. Chen; Y.H. Li
Hepatitis E virus; Liver; Pigs; Reverse transcription-polymerase chain reaction; Zoonosis

Hepatitis E is a form of endemic acute hepatitis found in humans in many countries worldwide and is caused by the hepatitis E Virus (HEV). Detection of HEV in pigs indicates that they may be carriers, possibly through zoonosis. The prevalence of HEV in pigs in Colombia is unknown. Studies in the US found that 11% of pig livers sold in grocery stores are contaminated with HEV. It is also ... more

C. Gutiérrez-Vergara; J. Quintero; J.F. Duarte; J.P. Suescún; A. López-Herrera
Mice; Phenylalanine; Reproductive performance; Teratogenesis

We evaluated the effects of phenylalanine on reproductive performance and teratogenesis in mice, as well as we assessed its protective effect in mice treated with an acute dose of cyclophosphamide. Animals were divided into 6 experimental groups (females N = 15/group, males N = 5/group): G1, the negative control group, phosphate-buffered saline; G2, the positive control group, 35 mg ... more

R.J. Oliveira; J.R. Pesarini; M.O. Mauro; L.S. Fronza; S.G. Victorelli; W.B. Cantero; M.C. Sena; A.C.M.B. Antoniolli
Erythrocytes; Mice; Micronuclei; Pterodon emarginatus

The present study aimed to assess the cytotoxic, genotoxic, and antigenotoxic activities of sucupira oil (Pterodon emarginatus), which is commonly used as an anti-rheumatic, analgesic, antimicrobial, anticercariae, and anti-inflammatory. We used the mouse bone marrow micronucleus test as an experimental model. The experimental groups, which consisted of 5 animals, was ... more

L.A. Assunção; S.R. Lemes; L.A. Araújo; C.R. Costa; L.G. Magalhães; K.K. Moura; P.R. Melo-Reis
Genetic polymorphism; Leptin; Leptin receptor; Obesity; β3-adrenergic receptor

Obesity is due to the combined effects of genes, environment, lifestyle, and the interactions of these factors. The adrenergic receptor β3 (β3-AR), leptin (LEP) and leptin receptor (LEPR) genes have been intensively evaluated in the search of variants that could be related to obesity and its cardiometabolic complications. The results of most of these studies have ... more

S.F.P. Duarte; E.A. Francischetti; V.A. Genelhu; P.H. Cabello; M.M.G. Pimentel
Aging; Apolipoprotein E; Brazilian-Japanese elderly; Cardiovascular risk; Longevity; Obesity

Study of immigrant populations may contribute to a better understanding of the epidemiology of diseases associated with the aging process. We examined the prevalence of cardiovascular risk factors, including apolipoprotein E (ApoE) polymorphism, in elderly subjects who were born in Japan, migrated to South Brazil and have lived in that region for over 40 years, versus a group of elderly, ... more

N. Terra; Y. Moriguchi; L. Bittencourt; R.S. Trois; J.E.C. Piccoli; I.B.M. Cruz
BMI; Leptin; MC4R gene; Obesity

Mutations in the melanocortin-4 receptor (MC4R) are associated with severe obesity, independent of their effect on cortisol or thyroid-stimulating hormone levels. We examined a morbidly obese male (BMI = 62 kg/m2) with a binge-eating disorder and eight family members for mutations in the MC4R gene and potential differences in leptin levels. Fifty healthy individuals served as ... more

S. Buchbinder; U. Bärtsch; M. Müller; M. Zorn; P.P. Nawroth; T. Schilling