Research Article

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12/21/2010
Biological control; Biomphalaria tenagophila; Hemocyte behavior; Schistosoma mansoni; Schistosomiasis

The relationships between schistosomiasis and its intermediate host, mollusks of the genus Biomphalaria, have been a concern for decades. It is known that the vector mollusk shows different susceptibility against parasite infection, whose occurrence depends on the interaction between the forms of trematode larvae and the host defense cells. These cells are called amebocytes or ... more

A.L.D. Oliveira; E.M. Zanotti-Magalhães; E.M. Zanotti-Magalhães; L.A. Magalhães; J.T. Ribeiro-Paes; P.M. Levada
10/13/2010
Biological control; PCR; Phytopathogenic fungi; Trichoderma

Seven fungal isolates were identified as pan-global Hypocrea/Trichoderma species, from section Trichoderma, on the basis of their morphology. These species were H. lixii/T. harzianum and H. orientalis/T. longibrachiatum. PCR-based markers with primer M13 (core sequence of phage M13) and internal-transcribed spacer sequences of ribosomal ... more

K.A. Abd-Elsalam; I. Almohimeed; M.A. Moslem; A.H. Bahkali
10/27/2014
Bacillus; Biological control; Endophytic; Fusarium oxysporum f. sp cubense; Musa spp

Endophytic microorganisms consist of fungi, bacteria, and actinomycetes that play important roles in the process of plant adaptation to the environment. Currently, the natural associations between microorganisms and plant species are being explored for a large number of biotechnological applications. In this study, 122 endophytic bacteria were isolated from 5 cultivars of Musa ... more

A. Souza; J.C. Cruz; N.R. Sousa; A.R.L. Procópio; G.F. Silva
10/19/2011
12S rRNA; Bos gaurus; COI; Cyt b; Malayan gaur; PCR

PCR has been extensively used for amplification of DNA sequences. We conducted a study to obtain the best amplification conditions for cytochrome b (Cyt b), cytochrome c oxidase I (COI) and 12S rRNA (12S) gene fragments of Malayan gaur mtDNA. DNA from seven Malayan gaur samples were extracted for PCR amplification. Various trials and combinations were tested to determine ... more

M.K.A. Rosli; A.S. Zamzuriada; S.M.F. Syed-Shabthar; M.C. Mahani; O. Abas-Mazni; B.M. Md-Zain
06/28/2011
COI; Phylogenetic analysis; Tegillarca granosa

Blood cockles are among the most economically important brackish water invertebrates found in Malaysia. However, our knowledge of blood cockle phylogeny and systematics is rudimentary, especially for the species Tegillarca granosa. It is unclear, for instance, whether the cockles occurring on the west coast of peninsular Malaysia constitute a single species, or multiple, ... more

S.Y. Chee; M.N. Devakie; M.N.Siti Azizah
09/19/2013
16S rRNA; COI; Mitochondrial DNA gene sequences; Molecular phylogeny; Octopodidae species

Octopus in the family Octopodidae (Mollusca: Cephalopoda) has been generally recognized as a "catch-all" genus. The monophyly of octopus species in China's coastal waters has not yet been studied. In this paper, we inferred the phylogeny of 11 octopus species (family Octopodidae) in China's coastal waters using nucleotide sequences of two mitochondrial DNA genes: cytochrome c ... more

Z.M. Lü; W.T. Cui; L.Q. Liu; H.M. Li; C.W. Wu
05/09/2014
16S rRNA; COI; Crustacean; Genetic polymorphism; Genetic variability; Macrobrachium

The genus Macrobrachium includes prawns, which are widely distributed in lakes, floodplains, and rivers in tropical and subtropical regions of South America. This genus presents nearly 210 known species with great ecological and economic importance. However, few studies are related to the biology of these crustaceans. In this study, we analyzed the genetic variability and ... more

A.L. Guerra; A.V.B. Lima; R.V.Lucato Júnior; M.C. Chiachio; F.G. Taddei; L. Castiglioni
06/30/2003
Colombia; Genetic structure; Isoenzymes; Population genetics; Psorophora columbiae; Psorophora toltecum

Two populations of the mosquito Psorophora columbiae from the central Andean area of Colombia and one population of Ps. toltecum from the Atlantic coast of Colombia were analyzed for 11 isoenzyme markers. Psorophora columbiae and Ps. toltecum are two of the main vectors of Venezuelan equine encephalitis. We found no conspicuous genetic differences between the two ... more

M. Ruiz-Garcia; D. Ramirez; F. Bello; D. Alvarez
09/09/2008
Amazona aestiva; bottleneck; Conservation; Genetic structure; Microsatellite

The blue-fronted Amazon (Amazona aestiva) is a widely distributed Neotropical parrot and one of the most captured parrots in nature to supply the illegal trade of wild animals. The objectives of the present study were to analyze the genetic structure of A. aestiva to identify management units and support conservation planning and to verified if A. aestiva populations ... more

K.C.E. Leite; G.H.F. Seixas; I. Berkunsky; R.G. Collevatti; R. Caparroz
07/08/2008
Bothrops moojeni; conservation genetics; Genetic structure; Genetic variability; RAPD; Vipers

Bothrops moojeni is an abundant venomous snake responsible for most of the snakebite cases in the Central region of Brazil and as a result of the anthropogenic habitat disturbance, such as the increase in extensive farming, the range of B. moojeni has been greatly fragmented. Here, we obtained genomic DNA from a total of 75 snakes belonging to four populations. Genetic ... more

N.C.L. Dutra; M.P.C. Telles; D.L. Dutra; N.J.Silva Júnior

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