Research Article

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Biodiversity conservation; Coefficient of similarity; genetic breeding; Genetic variability; Grouping analyses; Molecular marker

The genetic diversity and characteristics of commercial interest of Passiflora species make it useful to characterize wild germplasm, because of their potential use for fruit, ornamental and medicinal purposes. We evaluated genetic diversity, using RAPD markers, of 32 genotypes of Passiflora cincinnata collected from the wild in the region of Vitória da Conquista, Bahia ... more

C.B.M. Cerqueira-Silva; L.D.H.C.S. Conceição; E.S.L. Santos; C.B. Cardoso-Silva; A.S. Pereira; A.C. Oliveira; R.X. Corrêa
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Coefficient of similarity; Conservation; genetic breeding; Genetic variability; Grouping analyses; Molecular marker

In spite of the importance of and the considerable variability observed in Passiflora (Passifloraceae), little is known about the genetic diversity of most of the species of this genus. We evaluated the genetic diversity by RAPD markers in 18 genotypes of Passiflora trintae. The 15 primers generated 112 markers, 84% of which were polymorphic. The genetic distance ... more

C.B.M. Cerqueira-Silva; C.B. Cardoso-Silva; E.S.L. Santos; L.D.H.C.S. Conceição; A.S. Pereira; A.C. Oliveira; R.X. Corrêa
09/08/2010
Microsatellite; Molecular marker; Mugil cephalus

Using an (AG)13 enriched genomic library of Mugil cephalus, 12 polymorphic microsatellite loci were isolated and char­acterized in a test population; the number of alleles ranged from 2 to 11. The observed and expected heterozygosities ranged from 0.2593 to 0.8966 and from 0.3047 to 0.8454, respectively. Two loci deviated from Hardy-Weinberg equilibrium; linkage disequilibrium ... more

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Microsatellite; Molecular marker; Mytilus coruscus

Mytilus coruscus is one of the most important cultured species of marine shellfish in China. Using an expressed sequence tag-library and two microsatellite-enriched genomic libraries of M. coruscus, we isolated and characterized 12 polymorphic microsatellites in a test population; the number of alleles ranged from three to seven, and the observed and expected ... more

T.J. Xu; Y.N. Sun; Y.T. Yuan; Z. Liao; R.X. Wang
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Microsatellite; Miichthys miiuy; Molecular marker

Twelve polymorphic microsatellites from the (AG)13 and (CA)13 enriched genomic libraries of Miichthys miiuy were isolated and characterized in a test population; the number of alleles ranged from two to nine. The observed and expected heterozygosities ranged from 0.1923 to 1.0000 and from 0.2633 to 0.8337, respectively. Three loci deviated from Hardy- ... more

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Genetic variability; Molecular marker; Population variability; Silverleaf whitefly

The silverleaf whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae), is a cryptic species complex that contains some of the most damaging pests in tropical and subtropical regions. Recent studies have indicated that this complex is composed of at least 24 distinct and morphologically indistinguishable species that mainly differ in their ability to transmit phytoviruses, ... more

G.Edo Valle; A.L. Lourenção; M.I. Zucchi; J.B. Pinheiro; A.G. de Abreu
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Chloroplast; Fragaria vesca; Genome; Molecular marker; Simple sequence repeats

The use of simple sequence repeats (SSRs), or microsatellites, as genetic markers has become popular due to their abundance and variation in length among individuals. In this study, we investigated linkage groups (LGs) in the woodland strawberry (Fragaria vesca) and demonstrated variation in the abundances, densities, and relative densities of mononucleotide, dinucleotide, and ... more

L. Guan; J.F. Huang; G.Q. Feng; X.W. Wang; Y. Wang; B.Y. Chen; Y.S. Qiao
10/19/2012
Meat traits; Molecular marker; PPAR-γ gene; SNPs

The peroxisome proliferator-activated receptor gamma (PPAR-γ) is a key molecule in adipocyte differentitation; it transactivates multiple target genes in lipid metabolic pathways. Using PCR-SSCP and DNA sequencing, we evaluated a potential association of an SNP (72472 G﹥T in exon7) of the bovine PPAR-γgene with carcass and meat quality traits in 660 ... more

Y.Y. Fan; G.W. Fu; C.Z. Fu; L.S. Zan; W.Q. Tian
08/13/2012
Grapevine breeding; Marker assisted selection; Molecular marker; Seedlessness

We used molecular markers associated with seedlessness in grapes, namely SCC8, SCF27 and VMC7f2, to improve the efficiency of seedless grapevine breeding via marker assisted selection (MAS). DNA from 372 F1 hybrid progeny from the cross between seeded “Alphonse Lavallée” and seedless “Sultani” was amplified by PCR using three markers. After digestion of SCC8 marker ... more

M. Akkurt; A. Çakır; M. Shidfar; B.P. Çelikkol; G. Söylemezoğlu

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