Research Article

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Polymorphisms in genes encoding P450 cytochrome enzymes may increase the risk of sporadic colorectal cancer (SCRC). Here we investigated the association between SCRC and CYP2E1 (PstI) and CYP1A1 (MspI) polymorphisms in a case-control study. Moreover, we sought to determine any possible associations between this disease and the sociodemographic factors. ... more

M.A. Proença; G.M.M. Fernandes; A. Russo; R.B. Lelis; J.G. Netinho; G.S. Cunrath; A.E. Silva; E.M. Goloni-Bertollo; E.C. Pavarino
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Chronic obstructive pulmonary disease; Inflammatory response; Intercellular adhesion molecule-1; Interleukin-1b

Chronic obstructive pulmonary disease (COPD) is a devastating lung disorder characterized by sustained airway flow restriction that is not fully reversible. The precise pathogenic mechanisms are unknown, but it is clear that cigarette smoking and chronic inflammatory stimulation are the major causes of COPD. Lung inflammation associated with COPD involves multiple cytokines, aggregation ... more

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Fermentation; Protein; Proteomics; Zymomonas mobilis

Zymomonas mobilis has aroused considerable interest owing to its rapid metabolism and efficiency in producing ethanol and by-products such as levans, sorbitol, and gluconic acid from simple sugars. We performed a proteomic analysis of Z. mobilis UFPEDA241 to provide a global profile of regulatory proteins. The choice of the methods of extraction and cell lysis are ... more

D.R. Cavalcanti; C.B. Malafaia; T.D. Silva; B.S. Santos; G.M.T. Calazans; M.V. Silva
10/30/2015
cluster analysis; Faba bean; Genetic similarity; Protein; SDS-PAGE

To investigate genetic diversity and relationships of 101 faba bean (Vicia faba L.), landraces and varieties from different provinces of China and abroad were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). A total of 2625 unambiguous and stable bands from 101 germplasms were detected, and 36 different bands were classified according to the ... more

W.W. Hou; X.J. Zhang; J.B. Shi; Y.J. Liu
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Bolting; Chinese cabbage; Gene expression; Leaf color; Protein

Bolting and flowering are key processes during the growth and development of Chinese cabbage (Brassica rapa L. ssp pekinensis). Understanding the molecular mechanisms underlying bolting and flowering is of significance for improving production of the vegetable. A leaf-color change from bright green to gray-green has been observed following differentiation of the ... more

Y.W. Zhang; M.H. Guo; X.B. Tang; D. Jin; Z.Y. Fang; Y.W. Zhang; M.H. Guo; X.B. Tang; D. Jin; Z.Y. Fang
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Dinoprostone; mRNA; Prostaglandin receptors; Term pregnancy; Uterus

Prostaglandin E2 (dinoprostone) is largely used for labor induction. However, one-third of patients do not respond to treatment. One cause of this poor response may be associated with changes in regulation of prostaglandin E receptors (EP1-4). In this study, we investigated EP mRNA expression in the uterine cervix and lower uterine segment myometrium for term births. Biopsies were ... more

C.K. Konopka; W.G. Glanzner; M.L. Rigo; M.T. Rovani; F.V. Comim; P.B.D. Gonçalves; E.N. Morais; A.Q. Antoniazzi; C.F. Mello; I.B.M. Cruz
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Bronchial asthma; Children; Eosinophils; ECP; mRNA

Studies have shown that eosinophils are closely related to pathogenesis of bronchial asthma. Eosinophils release eosinophil cationic protein (ECP), which plays an important role in infection and allergic reactions. Serum ECP mRNA expression in children with bronchial asthma has not been adequately investigated. We analyzed serum ECP mRNA expression in 63 children with bronchial asthma and ... more

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Arbovirus; Dengue; DGCR8; mRNA; RNAi

Dengue virus (DENV) and its four serotypes (DENV1-4) belong to the Flavivirus genus of the Flaviviridae family. DENV infection is a life-threatening disease, which results in up to 20,000 deaths each year. Viruses have been shown to encode trans-regulatory small RNAs, or microRNAs (miRNAs), which bind to messenger RNA and negatively regulate host or viral gene expression. During ... more

S.M.M. Casseb; D.B. Simith; K.F.L. Melo; M.H. Mendonça; A.C.M. Santos; V.L. Carvalho; A.C.R. Cruz; P.F.C. Vasconcelos; S.M.M. Casseb; D.B. Simith; K.F.L. Melo; M.H. Mendonça; A.C.M. Santos; V.L. Carvalho; A.C.R. Cruz; P.F.C. Vasconcelos
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DNA; miRNA; mRNA; Next-generation sequencing; Phalaenopsis; PhalDB

The orchid family Orchidaceae is one of the largest families of angiosperms; it includes approximately 35,000 species, displays a wide range of unique flower shapes and is a valued ornamental crop. Among the various species of orchids, the Phalaenopsis genus is the most widely commercialized among blooming potted plants. Our aim was to provide in-depth ... more

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Gene expression; Hypoxia; Muscle; Pig

Hypoxia influences many physiological processes, such as respiration, cardiovascular, neurophysiology, and digestion. Skeletal muscle is an important motor organ, which relies on oxygen of oxidation; however, the study of hypoxia in skeletal muscle is lacking. In order to understand the effect of hypoxia on skeletal muscle, we determined the expression level of four hypoxia-related genes ( ... more

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