Research Article

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03/29/2007
Dalbergia species; DNA extraction; Herbarium specimens; PCR amplification

Five published DNA extraction protocols were compared for their ability to produce good quality DNA from fresh and herbarium leaves of several species of the genus Dalbergia. The leaves of these species contain high amounts of secondary metabolites, which make it difficult to perform a clean DNA extraction and thereby interfering with subsequent PCR amplification. The protocol ... more

R.A. Ribeiro; M.B. Lovato
07/13/2010
CTAB; DNA extraction; Herbarium specimen; Papilionoideae; PCR amplification

Three DNA extraction protocols were compared for their ability to yield DNA from the leaves of herbarium specimens of nine species from nine genera of the Papilionoideae. We tested two protocols that use classic procedures for lysis and purification with cetyl trimethylammonium bromide (CTAB); a third protocol used a Nucleospin Plant kit. DNA obtained from all three procedures was ... more

M. Riahi; S. Zarre; A.A. Maassoumi; F. Attar; K. Osaloo
03/09/2010
DNA; Essential oil; PCR amplification; RAPDs; Tagetes minuta

Tagetes, a genus of flowering marigolds in the family Asteraceae (Compositeae), is reported to be a medicinal plant with hypotensive, spasmolytic, anti-inflammatory, antimicrobial, and antifungal properties. Tagetes minuta characteristically contains high concentrations of essential oils, flavonoids, polyphenols, and polysaccharides that interfere with DNA, causing ... more

I. Shahzadi; R. Ahmed; A. Hassan; M.M. Shah
09/23/2013
Macaw palm; Molecular marker; Oleaginous species; Optimization DNA; PCR amplification; SSR

Macaw palm, Acrocomia aculeata is an oleaginous species of the Arecaceae family; it has been identified as one of the most promising plants for sustainable production of renewable energy, especially biodiesel. We developed an efficient protocol of genomic DNA extraction for A. aculeata using leaf and stipe tissues, based on the cationic hexadecyltrimethylammonium bromide ... more

E.C.M. Lanes; C. Nick; K.N. Kuki; R.D. Freitas; S.Y. Motoike
10/22/2013
Apis mellifera; DNA extraction; Lysis buffer; Polyacrylamide gel; Proteinase K; Varroa destructor

We developed a rapid method for extraction of DNA from honey bees, Apis mellifera, and from the parasitic bee mite, Varroa destructor. The advantages include fast processing and low toxicity of the substances that are utilized. We used lysis buffer with nonionic detergents to lyse cell walls and proteinase K to digest proteins. We tested whole thorax, thoracic muscle ... more

M.R.C. Issa; V.L.C. Figueiredo; D. De Jong; C.H. Sakamoto; Z.L.P. Simões
12/18/2014
DNA isolation; Fungi; Lyticase; Proteinase K

DNA isolation is a crucial step of conducting genetic studies in any organism. However, this process is quite difficult when studying fungi because of the need to damage the fungal cell walls of specific structures. In this study, we developed a method for the rapid and efficient isolation of fungal DNA based on simultaneous mechanical and enzymatic cell wall degradation. There are ... more

T. Lech; J. Syguła-Cholewinska; J. Szostak-Kot
09/21/2015
EGFR; FISH; Metastasis; NSCLC

The aim of this study was to explore epidermal growth factor receptor (EGFR) gene amplification and its relationship with cancer invasion and metastasis in non-small cell lung cancer (NSCLC). EGFR amplification in 45 patients with NSCLC and 15 subjects with normal lung tissues was detected by fluorescence in situ hybridization. The relationship between EGFR ... more

X.F. Jia; J. Li; H.B. Zhao; J. Liu; J.J. Liu
11/25/2015
chromosome; FISH; Multigene families

In this study, fluorescence in situ hybridization (FISH) analysis was used to determine and compare the numbers and chromosomal locations of two multigene families (rDNA and histone H3) in four Neotropical species of gomphocerine grasshoppers. FISH using the 18S rDNA probe identified a single site on the S9 chromosome of Amblytropidia sp and Cauratettix borelli, ... more

L.C. Silva-Neto; A.C.S. Bernardino; V. Loreto; R.C. Moura
05/09/2016
Array CGH; Balanced reciprocal translocation; DiGeorge syndrome; FISH; Karyotype; Velocariofacial syndrome

Partial duplication of the long arm of chromosome 11 and the partial trisomy of 22q are uncommon karyotypic abnormalities. Here, we report the case of a 6-year-old girl who showed partial trisomy of 11q and 22q, as a result of a maternal balanced reciprocal translocation (11;22), and exhibited dysmorphic features, severe intellectual disability, brain malformations, and speech delay ... more

P.S. Zou; H.F. Li; L.S. Chen; M. Ma; X.H. Chen; D. Xue; D.H. Cao; P.S. Zou; H.F. Li; L.S. Chen; M. Ma; X.H. Chen; D. Xue; D.H. Cao
02/20/2018
Array-SNP; FISH; Prenatal diagnosis; Trisomy 7q11.23q21.3; VSD

The objective of prenatal diagnosis (PD) is to provide prenatal diagnostic testing services for genetic conditions that enable families to make informed choices consistent with their individual needs and values, and to support them in deal with the outcome of such testing. This case we reported is about two fetuses with ventricular septal defect (VSD) and trisomy ... more

K. Mu; L.Sha Chen; J. Wen; Y. Liu; N. Liu; D.Hua Cao

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