Research Article

Related GMR Articles

10/29/2015

Expression and localization of Luman/CREB3 in mouse embryos during the pre-implantation period

Development; Early embryo; Luman; Mice; Oocyte

Luman/CREB3 is a transcription factor that is a member of the cAMP-response-element-binding protein family of basic region-leucine zipper transcription factors. This protein interacts with host cell factor 1, which also associates with the herpes simplex virus protein VP16 to induce the transcription of herpes simplex virus. Currently, the physiological function of Luman/CREB3 in ... more

T.N. Mahmoud; P.F. Lin; F.L. Chen; J.H. Zhou; X.G. Wang; N. Wang; X. Li; Y.P. Jin
10/19/2015

Arm-Gal4 inheritance influences development and lifespan in Drosophila melanogaster

Arm-Gal4; Development; Drosophila melanogaster; Longevity; Parental inheritance; UAS-lacZ

The UAS-Gal4 ectopic expression system is a widely used and highly valued tool that allows specific gene expression in Drosophila melanogaster. Yeast transcription factor Gal4 can be directed using D. melanogaster transcriptional control elements, and is often assumed to have little effect on the organism. By evaluation of the consequences of maternal and ... more

F.A. Slade; B.E. Staveley
10/24/2016

Development and characterization of novel EST-SSR markers and their application for genetic diversity analysis of Jerusalem artichoke (Helianthus tuberosus L.)

Development; EST-SSR markers; Genetic diversity; Jerusalem artichoke

Jerusalem artichoke (Helianthus tuberosus L.) is a perennial tuberous plant and a traditional inulin-rich crop in Thailand. It has become the most important source of inulin and has great potential for use in chemical and food industries. In this study, expressed sequence tag (EST)-based simple sequence repeat (SSR) markers were developed from 40,362 ... more

T. Mornkham; P.P. Wangsomnuk; X.C. Mo; F.O. Francisco; L.Z. Gao; H. Kurzweil; T. Mornkham; P.P. Wangsomnuk; X.C. Mo; F.O. Francisco; L.Z. Gao; H. Kurzweil; T. Mornkham; P.P. Wangsomnuk; X.C. Mo; F.O. Francisco; L.Z. Gao; H. Kurzweil; T. Mornkham; P.P. Wangsomnuk; X.C. Mo; F.O. Francisco; L.Z. Gao; H. Kurzweil
07/14/2015

Manipulation of primer affinity improves high-resolution melting accuracy for imprinted genes

Epigenetics; High-resolution melting; Imprinted genes; Methylation; Polymerase chain reaction

High-resolution melting (HRM) is considered an inexpensive, rapid, and attractive methodology for methylation analysis. In the application of the polymerase chain reaction (PCR) to methylation analysis, amplification efficiencies are biased towards unmethylated, rather than methylated, templates: a phenomenon known as PCR bias. To overcome PCR bias, primers that include CpG site(s) and ... more

F.V.M. Rubatino; N.V. Carobin; M.L. Freitas; V.T. de Oliveira; R.X. Pietra; P.P.R. Oliveira; A.A. Bosco; F.S. Jehee
11/26/2015

Methylation-sensitive amplified polymorphism-based genome-wide analysis of cytosine methylation profiles in Nicotiana tabacum cultivars

Epigenetics; Methylation; Methylation-sensitive amplified polymorphism; Nia1; Phenotype; Tobacco (Nicotiana tabacum)

This study aimed to investigate cytosine methylation profiles in different tobacco (Nicotiana tabacum) cultivars grown in China. Methylation-sensitive amplified polymorphism was used to analyze genome-wide global methylation profiles in four tobacco cultivars (Yunyan 85, NC89, K326, and Yunyan 87). Amplicons with methylated C motifs were cloned by reamplified polymerase chain ... more

J. Jiao; J. Wu; Z. Lv; C. Sun; L. Gao; X. Yan; L. Cui; Z. Tang; B. Yan; Y. Jia
08/19/2016

Epigenetic mechanism of maternal post-traumatic stress disorder in delayed rat offspring development: dysregulation of methylation and gene expression

Behavior; Environmental enrichment; Epigenetics; Neurodevelopment; Post-traumatic stress disorder

Maternal post-traumatic stress disorder (PTSD) increases the risk of adverse neurodevelopmental outcomes in the child. Epigenetic alternations may play an essential role in the negative effects of PTSD. This study was aimed to investigate the possible epigenetic alterations of maternal PTSD, which underpins the developmental and behavioral impact. 24 pregnant Sprague-Dawley (SD) rats ... more

X.G. Zhang; H. Zhang; X.L. Liang; Q. Liu; H.Y. Wang; B. Cao; J. Cao; S. Liu; Y.J. Long; W.Y. Xie; D.Z. Peng; X.G. Zhang; H. Zhang; X.L. Liang; Q. Liu; H.Y. Wang; B. Cao; J. Cao; S. Liu; Y.J. Long; W.Y. Xie; D.Z. Peng
10/14/2017

DNA methylation profile of the DKK2 gene as a biomarker in patients with colorectal cancer

Biomarkers; Colorectal cancer; DKK2 gene; DNA methylation; Epigenetics
Purpose: Epigenetic changes can be detected in precancerous lesions, suggesting that may be involved in the early stages of carcinogenesis. The methylation of specifics genes has been correlated with the outcome of many different types of cancers. This study compared the levels of DNA methylation between normal and tumor tissues from patients with colorectal ... more
T.D. Silva; A.V. Felipe; V.M. Vidigal; S.S. Saad; N.M. Forones