Research Article

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02/19/2016
Gene expression; peanut; Pod development; SQUAMOSA promoter-binding protein-like protein

SQUAMOSA promoter-binding protein-like (SPL) proteins play crucial roles in plant growth, development, and responses to environmental stressors. The peanut (Arachis hypogaea L.) is a globally important oil crop. In this study, we cloned the full-length cDNA of 15 SPLs in the peanut by transcriptome sequencing and rapid amplification of cDNA ends, and analyzed their ... more

M. Li; S.Z. Zhao; C.Z. Zhao; Y. Zhang; H. Xia; J. Lopez-Baltazar; S.B. Wan; X.J. Wang; M. Li; S.Z. Zhao; C.Z. Zhao; Y. Zhang; H. Xia; J. Lopez-Baltazar; S.B. Wan; X.J. Wang
10/24/2016
Bioinformatic analysis; Gene expression; MYB10; Red-leaf peach

In higher plants, the transcription factor MYB10 is an important regulator of anthocyanin biosynthesis. In order to study its role in the development of red coloration in peach leaves, the full-length MYB10 complementary DNA sequence of the red-leaf peach cultivar ‘Tsukuba No. 5’ (Prunus persica f. atropurpurea) was successfully cloned ... more

X.Q. Wang; J. Han; Y. Wen; W.B. Jiang; J.G. Fang; B.B. Zhang; R.J. Ma; X.Q. Wang; J. Han; Y. Wen; W.B. Jiang; J.G. Fang; B.B. Zhang; R.J. Ma
12/02/2016
Animals; Avian Proteins; Body weight; Breeding; Chickens; Female; Gene expression; Gene Expression Regulation; male; Muscle development; Myostatin; Organ Specificity; Phenotype; Quantitative trait loci

Myostatin (MSTN) is expressed in the myotome and developing skeletal muscles, and acts to regulate the number of muscle fibers. Wuding chicken large body, developed muscle, high disease resistance, and tender, delicious meat, and are not selected for fast growth. Broiler chickens (Avian broiler) are selected for fast growth and have a large body size and high muscle mass. Here, 240 one-day-old ... more

L.X. Liu; T.F. Dou; Q.H. Li; H. Rong; H.Q. Tong; Z.Q. Xu; Y. Huang; D.H. Gu; X.B. Chen; C.R. Ge; J.J. Jia
12/02/2016
Deoxyribonucleases; DNA; Gene expression; Plant Leaves; RNA, Plant; Saccharum

The success of gene expression studies, protein synthesis, and construction of cDNA libraries directly depends on the purity and integrity of the RNA used in these tests, as even minor amounts of contaminant DNA (<1%) can produce a false positive amplification signal in quantitative real-time PCR. For RNA contaminated with genomic DNA, an essential step in the studies on gene expression is ... more

J.A. Santos; G.A. Luz; K.P. Oliveira; L.F. Oliveira; A.S.Andrade Júnior; S.E.S. Valente; P.S.C. Lima
12/19/2016
Athletes; Cell Hypoxia; Cell proliferation; Cells, Cultured; Endothelial progenitor cells; Exercise; Gene expression; Humans; Lipoproteins; Skiing

In this study, we compared the functional properties of endothelial progenitor cells (EPCs) derived from halfpipe-snowboarding athletes who train under hyperoxic conditions with those derived from normal subjects who lived under normoxic conditions. Peripheral blood-derived EPCs were isolated from both halfpipe-snowboarding athletes and normal humans. Cellular growth dynamics, lipoprotein ... more

Y.H. Zhao; J.C. Kan; Y.F. Wang; W.J. Guan; Z.Q. Zhu
12/19/2016
Animals; Female; Forkhead Box Protein L2; Forkhead Transcription Factors; Gene expression; Goats; Hair follicle; Quantitative trait loci; Real-time polymerase chain reaction; Toll-Like Receptors; Wnt4 Protein

The mechanism of dominant follicle selection is unclear because of its physiological complexity. However, some studies have reported that the immune system plays an important role in reproductive physiology. The objective of the current study was to investigate the differential expression of Toll-like receptors (TLRs) in the dominant (DFs) and nondominant follicles (NFs), and to determine the ... more

G.X. E; Y.J. Zhao; Y.H. Ma; M.X. Chu; Q.H. Hong; S.J. Lv; L. Zhu; F.K. Li; Y.G. Han; X. Wu; Y.F. Huang
12/23/2016
Comparative genomic hybridization; DNA Copy Number Variations; Gamma rays; Gene expression; Genetic association studies; Mutation; Oryza; Phenotype

Comparative genomic hybridization (CGH) is a powerful tool used to analyze changes in copy number, polymorphisms, and structural variations in the genome. Gene copy number variation (CNV) is a common form of natural diversity in the genome, which can create new genes and alter gene structure. Thus, CNVs may influence phenotypic variation and gene expression. In this study, to detect CNVs, we ... more

J.E. Hwang; S.H. Kim; I.J. Jung; S.M. Han; J.W. Ahn; S.J. Kwon; S.H. Kim; S.Y. Kang; D.S. Kim; J.B. Kim
1/23/2017
Gene expression; Humans; Parkinson disease; Prion Proteins; REM Sleep Behavior Disorder; RNA, Messenger

Parkinson's disease (PD) is one of the most common neurodegenerative diseases and mainly manifests with decreasing numbers of dopaminergic neurons. Rapid eye movement (REM) sleep behavior disorder (RBD) has an incidence of 15-47% in all PD patients. Prion proteins (PrPs), which are expressed in both neurons and glial cells of the brain, are believed to be correlated with abnormal neurological ... more

W.J. Zhang; X.L. Shang; J. Peng; M.H. Zhou; W.J. Sun
1/23/2017
Animal Feed; Animals; Chickens; Female; Gene expression; Gene expression profiling; Meat Products; Muscles

Chicken meat quality is becoming increasingly important among breeders and consumers. To understand the effect of feeding conditions on chicken meat quality, we investigated the profiles of genes expressed in chicken breast muscle. Using RNA sequencing, we identified 336, 321, and 387 differentially expressed genes among Chengkou, Daninghe, and Qingjiaoma chickens under scatter- and captivity- ... more

G.L. Gao; C. Wang; X.Z. Zhao; H.W. Wang; Q. Li; J. Li; K.S. Zhang; H. Zhong; Q.G. Wang
2017 Jan 23
alpha-Defensins; Animals; Gene expression; Gene Transfer Techniques; Lactuca; Plants, Genetically Modified; Rabbits

Lettuce (Lactuca sativa L.) is an annual plant of the daisy family, Asteraceae, with high food and medicinal value. However, the crop is susceptible to several viruses that are transmitted by aphids and is highly vulnerable to post-harvest diseases, as well as insect and mammal pests and fungal and bacterial diseases. Here, the rabbit defensin gene NP-1 was transferred into lettuce by ... more

D. Song; X. Xiong; W.F. Tu; W. Yao; H.W. Liang; F.J. Chen; Z.Q. He

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