Research Article

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10/05/2007
Candida albicans; Glutathione reductase; GRX2; Virulence

Resistance of Candida albicans to reactive oxygen species is thought to enhance its virulence in mammalian hosts. Genes such as SOD1, which encodes the anti-oxidant, superoxide dismutase, are known virulence factors. We disrupted the gene GRX2, which encodes a putative glutathione reductase (glutaredoxin) in C. albicans, and we compared the mutant with an ... more

G.M. Chaves; D.M. MacCallum; F.C. Odds; S. Bates
11/09/2006
AAA ATPases; Candida albicans; CottonPrep; Differential screening; Insertional mutagenesis; RIX7; YLL34

Proper morphology is essential for the ability of Candida albicans to switch between yeast and hyphae and thereby sustain its virulence. Here we identified, by differential screening, a novel C. albicans AAA ATPase encoding gene, CaYLL34 (RIX7), with enhanced expression in hyphae. Phylogenetic analysis suggests that CaYLL34 belongs to a “VCP ... more

A.S.A. Melo; A.C.B. Padovan; R.C. Serafim; L. Puzer; A.K. Carmona; J. Neto; A. Brunstein; M.R.S. Briones
01/30/2015
25S rDNA-PCR; Candida albicans; Polymerase chain reaction; Random amplified polymorphic DNA

We analyzed the distribution of Candida albicans in the oral cavity of 3-5-year-old children of Uygur and Han nationalities as well as their genotypes in caries-active groups in the Urumqi municipality. CHROMagar Candida was separately cultivated, and we identified 359 Uygur and Han children aged 3-5 years. We randomly selected 20 Han children and 20 Uygur children for this ... more

N. Wu; J. Lin; L. Wu; J. Zhao
03/31/2006
Bayesian networks; Gene networks; Pheromone response pathway; Saccharomyces cerevisiae; Transcriptional regulation

Gene regulatory networks, or simply gene networks (GNs), have shown to be a promising approach that the bioinformatics community has been developing for studying regulatory mechanisms in biological systems. GNs are built from the genome-wide high-throughput gene expression data that are often available from DNA microarray experiments. Conceptually, GNs are (un)directed graphs, where ... more

D.Fernando Veiga; F.Fernandes Vicente; G. Bastos
02/12/2008
checkpoint; DNA repair; DNA replication; Proliferating cell nuclear antigen; Rad17/Mec3/Ddc1; Saccharomyces cerevisiae

DNA damage activates several mechanisms such as DNA repair and cell cycle checkpoints. The Saccharomyces cerevisiae heterotrimeric checkpoint clamp consisting of the Rad17, Mec3 and Ddc1 subunits is an early response factor to DNA damage and activates checkpoints. This complex is structurally similar to the proliferating cell nuclear antigen (PCNA), which serves as a sliding clamp ... more

J.M. Cardone; M. Brendel; J.A.P. Henriques
01/08/2008
budding index; mutagen sensitivity; Ribonucleotide reductase; Saccharomyces cerevisiae; stannous chloride

Ribonucleotide reductase (RNR) of the yeast Saccharomyces cerevisiae is a tetrameric protein complex, consisting of two large and two small subunits. The small subunits Y2 and Y4 form a heterodimer and are encoded by yeast genes RNR2 and RNR4, respectively. Loss of Y4 in yeast mutant rnr4Δ can be compensated for by up-regulated expression of Y2, and the formation of ... more

T.S. Basso; C. Pungartnik; M. Brendel
11/27/2014
DNA repair; Genome instability; Oxidative damage; petite colonies; Saccharomyces cerevisiae

Cassia angustifolia Vahl. (senna) is commonly used in self-medication and is frequently used to treat intestine constipation. A previous study involving bacteria and plasmid DNA suggested the possible toxicity of the aqueous extract of senna (SAE). The aim of this study was to extend the knowledge concerning SAE genotoxicity mechanisms because of its widespread use and its risks ... more

C.R. Silva; A. Caldeira-de-Araújo; A.C. Leitão; M. Pádula
06/11/2015
4-β-glucanase; Aspergillus niger; Endo-1; Episomal plasmid vector; Glycosylation; HO locus; Saccharomyces cerevisiae

Heterologous expression of Aspergillus niger endo-1,4-β-glucanase (ENG1) in Saccharomyces cerevisiae was tested both with an episomal plasmid vector (YEGAp/eng1) and a yeast vector capable of integration into the HO locus of the S. cerevisiae chromosome (pHO-GAPDH-eng1-KanMX4-HO). In both cases, eng1 gene ... more

S.M. Taipakova; I.T. Smekenov; M.K. Saparbaev; A.K. Bissenbaev
02/08/2017
Aspergillus fumigatus; DNA damage; Ethidium; Fluorescent Dyes; Saccharomyces cerevisiae

Invasive aspergillosis is a disease responsible for high mortality rates, caused mainly by Aspergillus fumigatus. The available drugs are limited and this disease continues to occur at an unacceptable frequency. Gene disruption is essential in the search for new drug targets. An efficient protocol for A. fumigatus gene disruption was described but it requires ethidium bromide, a genotoxic ... more

H.M.S. Canela; L.A. Takami; M.E.S. Ferreira
08/07/2015
Chinese Hwamei; Garrulax canorus; Leiothrichidae; Mitochondrial genome; Passeriformes

The Chinese Hwamei Garrulax canorus, a member of the family Leiothrichidae, is commonly found in central and southern China, northern Indochina, and on Hainan Island. In this study, we sequenced the complete mitochondrial genome of G. canorus. The circular mitochondrial genome is 17,785 bp in length and includes 13 protein-coding genes, 22 transfer RNA (tRNA) genes, and ... more

D.S. Chen; C.J. Qian; Q.Q. Ren; P. Wang; J. Yuan; L. Jiang; D. Bi; Q. Zhang; Y. Wang; X.Z. Kan

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